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Literature summary extracted from

  • Mayordomo, I.; Randez-Gil, F.; Prieto, J.A.
    Isolation, purification, and characterization of a cold-active lipase from Aspergillus nidulans (2000), J. Agric. Food Chem., 48, 105-109.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.3 0.3
-
 4-nitrophenyl palmitate pH 6.5, 40°C Aspergillus nidulans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.3 extracellular culture medium Aspergillus nidulans
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.3 29000
-
 gel filtration Aspergillus nidulans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.3 4-nitrophenyl palmitate + H2O Aspergillus nidulans
-
 4-nitrophenol + palmitate
-
 ?
3.1.1.3 4-nitrophenyl palmitate + H2O Aspergillus nidulans WG312
-
 4-nitrophenol + palmitate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.3 Aspergillus nidulans
-
 cultured with olive oil as carbon source or in rich medium
-
3.1.1.3 Aspergillus nidulans WG312
-
 cultured with olive oil as carbon source or in rich medium
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.1.3 no glycoprotein
-
 Aspergillus nidulans

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.3 purified from culture medium, to homogeneity, 1186fold Aspergillus nidulans

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.3 additional information
-
 activity at different culture medium conditions Aspergillus nidulans
3.1.1.3 355.7
-
 purified enzyme Aspergillus nidulans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.3 4-nitrophenyl palmitate + H2O
-
 Aspergillus nidulans 4-nitrophenol + palmitate
-
 ?
3.1.1.3 4-nitrophenyl palmitate + H2O
-
 Aspergillus nidulans WG312 4-nitrophenol + palmitate
-
 ?
3.1.1.3 additional information substrate specificity Aspergillus nidulans ?
-
 ?
3.1.1.3 additional information substrate specificity Aspergillus nidulans WG312 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.3 monomer 1 * 29000, SDS-PAGE Aspergillus nidulans

Synonyms

EC Number Synonyms Comment Organism
3.1.1.3 lipase
-
 Aspergillus nidulans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.3 40
-
-
 Aspergillus nidulans

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.1.3
-
 20 cold-adapted enzyme, high activity in the range 0-20°C Aspergillus nidulans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.3 additional information
-
 cold-adapted enzyme shows reduced thermal stability Aspergillus nidulans

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.1.3 494
-
 4-nitrophenyl palmitate pH 6.5, 40°C Aspergillus nidulans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.3 6.5
-
-
 Aspergillus nidulans

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.1.1.3 Aspergillus nidulans isoelectric focusing
-
 4.85