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Literature summary extracted from

  • Ramirez, E.C.; Whitaker, J.R.
    Biochemical characterization of cystine lyase from broccoli (Brassica oleracea var. italica) (1999), J. Agric. Food Chem., 47, 2218-2225.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.4.1.13 DL-homocysteine 12.5 mM, 70% inhibition, competitive inhibitor Brassica oleracea
4.4.1.13 hydroxylamine 12.5 mM, 97% inhibition Brassica oleracea
4.4.1.13 L-cysteine 12 mM, 65% inhibition after 5 min, 1 mM, 29% inhibition after 10 min, linear noncompetitive inhibitor Brassica oleracea
4.4.1.13 NEM 1.25 mM, about 45% inhibition Brassica oleracea
4.4.1.13 pyridoxal 12.5 mM, in presence of 12 mM L-cystine 12% inhibition, in presence of 1 mM L-cystine 37% inhibition Brassica oleracea
4.4.1.13 pyruvate 12.5 mM, 25% inhibition Brassica oleracea
4.4.1.13 Sodium cyanide 12.5 mM, 95% inhibition Brassica oleracea
4.4.1.35 cyanide 12.5 mM, 5% residual activity Brassica oleracea var. italica
4.4.1.35 DL-homocysteine 2.5 mM, 59% residual activity Brassica oleracea var. italica
4.4.1.35 hydroxylamine 12.5 mM, 3% residual activity Brassica oleracea var. italica
4.4.1.35 L-cysteine linear noncompetitive inhibitor Brassica oleracea var. italica
4.4.1.35 pyridoxal 2.5 mM, 63% residual activity Brassica oleracea var. italica

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.4.1.13 0.0812
-
 L-cystine
-
 Brassica oleracea
4.4.1.35 0.0812
-
 L-cystine pH 8.4, 30°C Brassica oleracea var. italica

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.4.1.13 48000
-
 2 * 48000, SDS-PAGE Brassica oleracea
4.4.1.13 100000
-
 gel filtration Brassica oleracea
4.4.1.35 47000
-
-
 Brassica oleracea var. italica
4.4.1.35 48000
-
-
 Brassica oleracea var. italica
4.4.1.35 94000
-
 MALDI-TOF Brassica oleracea var. italica
4.4.1.35 100000 110000 gel filtration Brassica oleracea var. italica

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.4.1.13 additional information Brassica oleracea the enzyme is responsible for off-aroma deterioration in fresh unblanched broccoli ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.4.1.13 Brassica oleracea
-
 var. italica
-
4.4.1.35 Brassica oleracea var. italica
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
4.4.1.13 glycoprotein carbohydrate content of 3% Brassica oleracea

Purification (Commentary)

EC Number Purification (Comment) Organism
4.4.1.13
-
 Brassica oleracea
4.4.1.35
-
 Brassica oleracea var. italica

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.4.1.13 7.69
-
-
 Brassica oleracea
4.4.1.35 7.7
-
 pH 8.4, 30°C Brassica oleracea var. italica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.4.1.13 cystathionine + H2O 1-4% of the activity with L-cysteine, depending on assay method Brassica oleracea L-homocysteine + NH3 + pyruvate
-
 ?
4.4.1.13 D-cystine + H2O 2% of the activity with L-cystine Brassica oleracea ?
-
 ?
4.4.1.13 L-cysteine S-sulfate + H2O 11% of the activity with L-cystine Brassica oleracea ?
-
 ?
4.4.1.13 L-cystine + H2O
-
 Brassica oleracea L-thiocysteine + pyruvate + NH3
-
 ?
4.4.1.13 L-djenkolic acid + H2O 18-24% of the activity with L-cystine, depending on assay method Brassica oleracea ?
-
 ?
4.4.1.13 additional information the enzyme is responsible for off-aroma deterioration in fresh unblanched broccoli Brassica oleracea ?
-
 ?
4.4.1.13 S-ethyl-L-cysteine + H2O 18% of the activity with L-cystine Brassica oleracea ?
-
 ?
4.4.1.13 S-ethyl-L-cysteine sulfoxide + H2O 71% of the activity with L-cystine Brassica oleracea ?
-
 ?
4.4.1.13 S-methyl-L-cysteine + H2O 13-29% of the activity with L-cystine, depending on assay method Brassica oleracea ?
-
 ?
4.4.1.13 S-propyl-L-cysteine + H2O 29% of the activity with L-cystine Brassica oleracea ?
-
 ?
4.4.1.35 L-cysteine S-sulfate + H2O
-
 Brassica oleracea var. italica ?
-
 ?
4.4.1.35 L-cystine + H2O
-
 Brassica oleracea var. italica L-thiocysteine + pyruvate + NH3 overall reaction ?
4.4.1.35 L-djenkolic acid + H2O 18-24% of the activity with L-cystine Brassica oleracea var. italica ?
-
 ?
4.4.1.35 additional information negligible activity with L-cystathionine Brassica oleracea var. italica ?
-
 ?
4.4.1.35 S-ethyl L-cysteine + H2O 71% of the activity with L-cystine Brassica oleracea var. italica ?
-
 ?
4.4.1.35 S-methyl L-cysteine + H2O 29% of the activity with L-cystine Brassica oleracea var. italica ?
-
 ?
4.4.1.35 S-propyl L-cysteine + H2O 29% of the activity with L-cystine Brassica oleracea var. italica ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.4.1.13 dimer 2 * 48000, SDS-PAGE Brassica oleracea
4.4.1.35 dimer 2 * 47000, MALDI-TOF, 2 * 48000, SDS-PAGE Brassica oleracea var. italica

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.4.1.13 35
-
-
 Brassica oleracea
4.4.1.35 35
-
-
 Brassica oleracea var. italica

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.4.1.13 45
-
 10 min, completely stable up to Brassica oleracea
4.4.1.13 50
-
 10 min, 20% loss of activity Brassica oleracea
4.4.1.13 60
-
 10 min, 75% loss of actrivity Brassica oleracea
4.4.1.35 45
-
 10 min, stable Brassica oleracea var. italica

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.4.1.13 9.1
-
-
 Brassica oleracea
4.4.1.35 9.1
-
-
 Brassica oleracea var. italica

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.4.1.13 8.4 9.9 pH 8.4: about 55% of maximal activity, pH 9.9: about 30% of maximal activity Brassica oleracea

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
4.4.1.35 3
-
 complete loss of activity Brassica oleracea var. italica
4.4.1.35 4 8 30°C, stable for 24 h Brassica oleracea var. italica

Cofactor

EC Number Cofactor Comment Organism Structure
4.4.1.13 pyridoxal 5'-phosphate contains 2 mol per mol of enzyme Brassica oleracea
4.4.1.35 pyridoxal 5'-phosphate
-
 Brassica oleracea var. italica

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.4.1.13 1.5
-
 DL-homocysteine
-
 Brassica oleracea
4.4.1.13 5
-
 L-cysteine
-
 Brassica oleracea
4.4.1.35 1.5
-
 L-cysteine pH 8.4, 30°C Brassica oleracea var. italica