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Literature summary extracted from
- Purification and characterisation of a novel cysteine conjugate beta-lyase from the tapeworm Moniezia expansa (2000), Int. J. Parasitol., 30, 567-571.
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 4.4.1.13 | cytosol | - |
Monieza expansa | 5829 | - |
| 4.4.1.13 | mitochondrion | - |
Monieza expansa | 5739 | - |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 4.4.1.13 | 72000 | - |
gel filtration | Monieza expansa |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.4.1.13 | Monieza expansa | - |
tapeworm | - |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 4.4.1.13 | 0.037 | - |
- |
Monieza expansa |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 4.4.1.13 | -20°C, partially purified enzyme, 4 weeks, no loss of activity | Monieza expansa |
| 4.4.1.13 | 4°C, partially purified enzyme ,1 week, 41% loss of activity | Monieza expansa |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.4.1.13 | alpha-keto-gamma-methiobutyric acid + L-aspartate | transamination reaction | Monieza expansa | ? | - |
? |  |
| 4.4.1.13 | S-(1,1,2-trichlorovinyl)-L-cysteine | - |
Monieza expansa | ? | - |
? | |
| 4.4.1.13 | S-(E-1,2-Dichlorovinyl)-L-cysteine | - |
Monieza expansa | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 4.4.1.13 | monomer | 1 * 65000-70000, SDS-PAGE | Monieza expansa |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.4.1.13 | 37 | - |
- |
Monieza expansa |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.4.1.13 | 67 | - |
5 min, complete loss of activity | Monieza expansa |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.4.1.13 | 8.6 | - |
- |
Monieza expansa |
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Release 2023.1 (January 2023)
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