EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.1.1.3 | 2-mercaptoethanol | strong inhibition | Micrococcus sp. | |
3.1.1.3 | Co2+ | - |
Micrococcus sp. | |
3.1.1.3 | Cu2+ | - |
Micrococcus sp. | |
3.1.1.3 | cysteine | - |
Micrococcus sp. | |
3.1.1.3 | dithiothreitol | strong inhibition | Micrococcus sp. | |
3.1.1.3 | EDTA | strong inhibition | Micrococcus sp. | |
3.1.1.3 | Fe2+ | - |
Micrococcus sp. | |
3.1.1.3 | Mn2+ | slight inhibition | Micrococcus sp. | |
3.1.1.3 | additional information | no inhibition by PMSF, E-64, 1,10-phenanthroline, and tosyllysylchloromethyl ketone | Micrococcus sp. | |
3.1.1.3 | NaCl | loss of 50% activity at 7.5% | Micrococcus sp. | |
3.1.1.3 | Ni2+ | - |
Micrococcus sp. | |
3.1.1.3 | Zn2+ | - |
Micrococcus sp. |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.1.3 | 0.06 | - |
alpha-naphthyl caprylate | pH 7.5, 30°C | Micrococcus sp. | |
3.1.1.3 | 0.08 | - |
alpha-naphthyl butyrate | pH 7.5, 30°C | Micrococcus sp. | |
3.1.1.3 | 0.6 | - |
tricaprylin | pH 7.5, 30°C | Micrococcus sp. | |
3.1.1.3 | 5 | - |
tributyrin | pH 7.5, 30°C | Micrococcus sp. |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.1.1.3 | extracellular | secretion to the culture medium | Micrococcus sp. | - |
- |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.1.1.3 | additional information | not affected by Mg2+, Ca2+, K+, Na+ | Micrococcus sp. |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.1.1.3 | 44600 | - |
x * 44600, SDS-PAGE | Micrococcus sp. |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.1.3 | Micrococcus sp. | - |
- |
- |
3.1.1.3 | Micrococcus sp. INIA 528 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.1.3 | from culture supernatant to homogeneity, 296fold | Micrococcus sp. |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.1.1.3 | 239.5 | - |
purified enzyme | Micrococcus sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.1.3 | alpha-naphthyl butyrate + H2O | preferred substrate | Micrococcus sp. | alpha-naphthol + butyrate | - |
? | |
3.1.1.3 | alpha-naphthyl butyrate + H2O | preferred substrate | Micrococcus sp. INIA 528 | alpha-naphthol + butyrate | - |
? | |
3.1.1.3 | alpha-naphthyl caprylate + H2O | 58% of the activity with the alpha-isomer | Micrococcus sp. | alpha-naphthol + caprylate | - |
? | |
3.1.1.3 | alpha-naphthyl caprylate + H2O | 58% of the activity with the alpha-isomer | Micrococcus sp. INIA 528 | alpha-naphthol + caprylate | - |
? | |
3.1.1.3 | alpha-naphthyl laurate + H2O | - |
Micrococcus sp. | alpha-naphthol + laurate | - |
? | |
3.1.1.3 | alpha-naphthyl stearate + H2O | equally active with both isomers | Micrococcus sp. | alpha-naphthol + stearate | - |
? | |
3.1.1.3 | beta-naphthyl butyrate + H2O | 58% of the activity with the alpha-isomer | Micrococcus sp. | beta-naphthol + butyrate | - |
? | |
3.1.1.3 | beta-naphthyl caprylate + H2O | - |
Micrococcus sp. | beta-naphthol + caprylate | - |
? | |
3.1.1.3 | beta-naphthyl laurate + H2O | 62.5% of the activity with the alpha-isomer | Micrococcus sp. | beta-naphthol + laurate | - |
? | |
3.1.1.3 | beta-naphthyl stearate + H2O | equally active with both isomers | Micrococcus sp. | beta-naphthol + stearate | - |
? | |
3.1.1.3 | additional information | the enzyme hydrolyzes alpha-naphthyl esters of fatty acid with chain lengths from C4 to C18 | Micrococcus sp. | ? | - |
? | |
3.1.1.3 | additional information | the enzyme hydrolyzes alpha-naphthyl esters of fatty acid with chain lengths from C4 to C18 | Micrococcus sp. INIA 528 | ? | - |
? | |
3.1.1.3 | tributyrin + H2O | best substrate | Micrococcus sp. | dibutyrin + butyrate | - |
? | |
3.1.1.3 | tributyrin + H2O | best substrate | Micrococcus sp. INIA 528 | dibutyrin + butyrate | - |
? | |
3.1.1.3 | tricaprylin + H2O | - |
Micrococcus sp. | dicaprylin + caprylate | - |
? | |
3.1.1.3 | trilaurin + H2O | - |
Micrococcus sp. | dilaurin + laurate | - |
? | |
3.1.1.3 | trilaurin + H2O | - |
Micrococcus sp. INIA 528 | dilaurin + laurate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.1.1.3 | ? | x * 44600, SDS-PAGE | Micrococcus sp. |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.1.3 | tributyrin esterase | - |
Micrococcus sp. |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.1.3 | 30 | - |
- |
Micrococcus sp. |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.1.3 | 20 | 40 | 80% remaining activity | Micrococcus sp. |
3.1.1.3 | 50 | - |
5 min, 50% remaining activity | Micrococcus sp. |
3.1.1.3 | 60 | 70 | less than 20% activity after 2 min | Micrococcus sp. |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.1.1.3 | 7.5 | - |
- |
Micrococcus sp. |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.1.1.3 | 6 | 8.5 | rapid decrease in activity below pH 6.0 and above pH 8.5 | Micrococcus sp. |