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Literature summary extracted from

  • Hinton, A.C.; Hammock, B.D.
    Juvenile hormone esterase (JHE) from Tenebrio molitor: full-length cDNA sequence, in vitro expression, and characterization of the recombinant protein (2003), Insect Biochem. Mol. Biol., 33, 477-487.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.59 from fat bodies of pupae, full-length cDNA, expression in Hi-5 cells from Trichoplusia ni, sequencing Tenebrio molitor

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.59 1,1,1-trifluoro-3-(octane-1-sulfonyl)propan-2,2-diol IC50: 0.0016 mM Tenebrio molitor
3.1.1.59 1,1,1-trifluoro-3-decylsulfanyl-propan-2-one potent inhibitor, IC50: 3.6 nm Tenebrio molitor
3.1.1.59 1,1,1-trifluoro-3-octylsulfanyl-propan-2-one potent inhibitor, IC50: 4.6 nm Tenebrio molitor
3.1.1.59 Carbaryl weak, IC50: above 0.1 mM Tenebrio molitor
3.1.1.59 methamidophos weak, IC50: above 1 mM Tenebrio molitor
3.1.1.59 O-ethyl-S-phenyl-phosphoramidothiolate IC50: 0.0084 mM Tenebrio molitor
3.1.1.59 Phenylmethylsulfonylfluoride mediocre inhibitor, IC50: 0.24 mM Tenebrio molitor

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.59 0.000315
-
 juvenile hormone III recombinant JHE Tenebrio molitor
3.1.1.59 0.0191
-
 methyl 1-heptylthioacetothioate recombinant JHE Tenebrio molitor

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.59 soluble recombinant JHE Tenebrio molitor
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.59 63600
-
 1 * 66000, recombinant JHE, SDS-PAGE, 1 * 63600, recombinant JHE, sequence calculation Tenebrio molitor
3.1.1.59 66000
-
 1 * 66000, recombinant JHE, SDS-PAGE, 1 * 63600, recombinant JHE, sequence calculation Tenebrio molitor

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.59 juvenile hormone + H2O Tenebrio molitor selective enzyme, juvenile hormone metabolism, alters the activity of juvenile hormone, which regulates development and reproduction ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.59 Tenebrio molitor Q8WRD8
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.59 recombinant JHE, partial, 2.5fold Tenebrio molitor

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.59 fat body of 0-48 hours old pupae Tenebrio molitor
-
3.1.1.59 hemolymph the cDNA contains an independent secretory signal peptide and is likely to represent a specific isoform that would be present in the hemolymph in vivo Tenebrio molitor
-
3.1.1.59 pupa 0-48 hours old, fat bodies Tenebrio molitor
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.59 0.9
-
 recombinant JHE, hydrolysis of alpha-naphthyl acetate Tenebrio molitor
3.1.1.59 1.1
-
 recombinant JHE, hydrolysis of juvenile hormone III Tenebrio molitor
3.1.1.59 5.3
-
 recombinant JHE, hydrolysis of p-nitrophenyl acetate Tenebrio molitor
3.1.1.59 15.1
-
 recombinant JHE, hydrolysis of HEPTAT Tenebrio molitor

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.59 alpha-naphthyl acetate + H2O
-
 Tenebrio molitor alpha-naphthol + acetate
-
 ?
3.1.1.59 HEPTAT + H2O synthetic juvenile hormone analog Tenebrio molitor ?
-
 ?
3.1.1.59 juvenile hormone + H2O selective enzyme, juvenile hormone metabolism, alters the activity of juvenile hormone, which regulates development and reproduction Tenebrio molitor ?
-
 ?
3.1.1.59 juvenile hormone III + H2O hydrolyzes the methyl ester of juvenile hormone, highest specificity for juvenile hormone III Tenebrio molitor ?
-
 ?
3.1.1.59 juvenile hormone III + H2O
-
 Tenebrio molitor juvenile hormone III acid + methanol
-
 ?
3.1.1.59 methyl 1-heptylthioacetothioate + H2O HEPTAT Tenebrio molitor ?
-
 ?
3.1.1.59 p-nitrophenyl acetate + H2O
-
 Tenebrio molitor p-nitrophenol + acetate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.59 monomer 1 * 66000, recombinant JHE, SDS-PAGE, 1 * 63600, recombinant JHE, sequence calculation Tenebrio molitor

Synonyms

EC Number Synonyms Comment Organism
3.1.1.59 JHE
-
 Tenebrio molitor

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.59 5
-
 the activity decreases significantly below pH 5 Tenebrio molitor
3.1.1.59 5.2 10.8 active at a wide range of pH, at least 80% of the maximum activity in the pH-range Tenebrio molitor

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.1.1.59 Tenebrio molitor recombinant JHE, isoelectric focusing, pH-gradient: pH 3-7
-
 4.9
3.1.1.59 Tenebrio molitor recombinant JHE, theoretical pI
-
 5.3

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.1.1.59 0.0000036
-
 potent inhibitor, IC50: 3.6 nm Tenebrio molitor 1,1,1-trifluoro-3-decylsulfanyl-propan-2-one
3.1.1.59 0.0000046
-
 potent inhibitor, IC50: 4.6 nm Tenebrio molitor 1,1,1-trifluoro-3-octylsulfanyl-propan-2-one
3.1.1.59 0.0016
-
 IC50: 0.0016 mM Tenebrio molitor 1,1,1-trifluoro-3-(octane-1-sulfonyl)propan-2,2-diol
3.1.1.59 0.0084
-
 IC50: 0.0084 mM Tenebrio molitor O-ethyl-S-phenyl-phosphoramidothiolate
3.1.1.59 0.1
-
 weak, IC50: above 0.1 mM Tenebrio molitor Carbaryl
3.1.1.59 0.24
-
 mediocre inhibitor, IC50: 0.24 mM Tenebrio molitor Phenylmethylsulfonylfluoride
3.1.1.59 1
-
 weak, IC50: above 1 mM Tenebrio molitor methamidophos