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Literature summary extracted from

  • Haubruge, E.; Amichot, M.; Cuany, A.; Berge, J.B.; Arnaud, L.
    Purification and characterization of a carboxylesterase involved in malathion-specific resistance from Tribolium castaneum (Coleoptera: Tenebrionidae) (2002), Insect Biochem. Mol. Biol., 32, 1181-1190.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.1 additional information toxic effects of insecticides on the 2 strains: malathion, malaoxon, fenitrothion, primiphos-methyl, DDT, and deltamethrine Tribolium castaneum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.1 0.00001
-
 Alpha-naphthyl acetate non-specific enzyme, pH 6.9, 25°C Tribolium castaneum
3.1.1.1 0.0001
-
 Alpha-naphthyl acetate malathion-specific enzyme, pH 6.9, 25°C Tribolium castaneum
3.1.1.1 0.0015
-
 malathion malathion-specific enzyme, pH 6.9, 25°C Tribolium castaneum
3.1.1.1 0.026
-
 malathion non-specific enzyme, pH 6.9, 25°C Tribolium castaneum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.1 cytosol major part of malathion carboxylesterase Tribolium castaneum 5829
-
3.1.1.1 microsome
-
 Tribolium castaneum
-
-
3.1.1.1 mitochondrion
-
 Tribolium castaneum 5739
-
3.1.1.1 additional information subcellular distribution, overview Tribolium castaneum
-
-
3.1.1.1 nucleus
-
 Tribolium castaneum 5634
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.1 61700
-
 malathion carboxylesterase, gel filtration Tribolium castaneum
3.1.1.1 62000
-
 non-specific carboxylesterase, gel filtration Tribolium castaneum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.1 malathion + H2O Tribolium castaneum
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.1 Tribolium castaneum
-
 flour beetle, 2 strains, one is resistant to insecticide malathion possissing a malathion carboxylesterase, the other is suceptible to malathion possessing a non-specific carboxylesterase
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.1 1774.4fold, to homogeneity, from both strains Tribolium castaneum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.1 0.124
-
 purified malathion carboxylesterase, substrate malathion Tribolium castaneum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.1 alpha-naphthyl acetate + H2O preferred substrate for the enzyme from the malathion-suceptible strain Tribolium castaneum alpha-naphthol + acetate
-
 ?
3.1.1.1 malathion + H2O
-
 Tribolium castaneum ?
-
 ?
3.1.1.1 malathion + H2O preferred substrate for the malathion esterase from the malathion-resistant strain Tribolium castaneum ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.1 monomer 1 * 62000, about, both enzyme forms, SDS-PAGE Tribolium castaneum

Synonyms

EC Number Synonyms Comment Organism
3.1.1.1 malathion carboxylesterase
-
 Tribolium castaneum
3.1.1.1 MCE
-
 Tribolium castaneum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.1 25
-
 assay at Tribolium castaneum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.1.1 1.8
-
 Alpha-naphthyl acetate malathion-specific enzyme, pH 6.9, 25°C Tribolium castaneum
3.1.1.1 2.8
-
 malathion non-specific enzyme, pH 6.9, 25°C Tribolium castaneum
3.1.1.1 12.1
-
 Alpha-naphthyl acetate non-specific enzyme, pH 6.9, 25°C Tribolium castaneum
3.1.1.1 13
-
 malathion malathion-specific enzyme, pH 6.9, 25°C Tribolium castaneum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.1 6.9
-
 assay at Tribolium castaneum

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.1.1.1 Tribolium castaneum isoelectric focusing, enzyme form of malathion-sensitive insects
-
 6.6
3.1.1.1 Tribolium castaneum isoelectric focusing, enzyme form of malathion-resistant insects 7.4 7.3