Literature summary extracted from
Velayudhan, J.; Jones, M.A.; Barrow, P.A.; Kelly, D.J.
L-serine catabolism via an oxygen-labile L-serine dehydratase is essential for colonization of the avian gut by Campylobacter jejuni (2004), Infect. Immun., 72, 260-268.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
4.3.1.17 |
expression in Escherichia coli |
Campylobacter jejuni |
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
4.3.1.17 |
D-serine |
100 mM, 40% inhibition |
Campylobacter jejuni |
|
4.3.1.17 |
L-cysteine |
50 mM, 95% inhibition |
Campylobacter jejuni |
|
4.3.1.17 |
L-threonine |
100 mM, 56% inhibition |
Campylobacter jejuni |
|
KM Value [mM]
EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Comment |
Organism |
Structure |
---|
4.3.1.17 |
14.6 |
- |
L-serine |
- |
Campylobacter jejuni |
|
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
4.3.1.17 |
Fe2+ |
iron-sulfur enzyme |
Campylobacter jejuni |
|
Molecular Weight [Da]
EC Number |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
---|
4.3.1.17 |
51758 |
- |
2 * 51758, electrospray mass spectrometry |
Campylobacter jejuni |
4.3.1.17 |
123000 |
- |
gel filtration |
Campylobacter jejuni |
Natural Substrates/ Products (Substrates)
EC Number |
Natural Substrates |
Organism |
Comment (Nat. Sub.) |
Natural Products |
Comment (Nat. Pro.) |
Rev. |
Reac. |
---|
4.3.1.17 |
L-serine |
Campylobacter jejuni |
- |
pyruvate + NH3 |
- |
? |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
4.3.1.17 |
Campylobacter jejuni |
- |
- |
- |
Oxidation Stability
EC Number |
Oxidation Stability |
Organism |
---|
4.3.1.17 |
purified SdaA is inactivated with a half-life of approx. 1.5 h upon exposure to air, inactivated SdaA can be reactivated to approx. 60% of its activity under strict anaerobic conditiones with Fe2+ and dithiothreitol |
Campylobacter jejuni |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
4.3.1.17 |
recombinant His-tagged SdaA |
Campylobacter jejuni |
Source Tissue
EC Number |
Source Tissue |
Comment |
Organism |
Textmining |
---|
Specific Activity [micromol/min/mg]
EC Number |
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Comment |
Organism |
---|
4.3.1.17 |
115 |
- |
recombinant SdaA |
Campylobacter jejuni |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
4.3.1.17 |
L-serine |
- |
Campylobacter jejuni |
pyruvate + NH3 |
- |
? |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
4.3.1.17 |
dimer |
2 * 51758, electrospray mass spectrometry |
Campylobacter jejuni |
Cofactor
EC Number |
Cofactor |
Comment |
Organism |
Structure |
---|
4.3.1.17 |
additional information |
pyridoxal 5'-phosphate independent |
Campylobacter jejuni |
|