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Literature summary extracted from

  • Contreras-Rodriguez, A.; Ramirez-Zavala, B.; Contreras, A.; Schurig, G.G.; Sriranganathan, N.; Lopez-Merino, A.
    Purification and characterization of an immunogenic aminopeptidase of Brucella melitensis (2003), Infect. Immun., 71, 5238-5244.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.11.2 1,10-phenanthroline 80% inhibition at 0.01 mM Brucella melitensis
3.4.11.2 bestatin 55% inhibition at 0.25 mM Brucella melitensis
3.4.11.2 EDTA complete inhibition at 2.5 mM Brucella melitensis
3.4.11.2 Hg2+ complete inhibition at 1 mM Brucella melitensis
3.4.11.2 Mg2+ 14% inhibition at 1 mM Brucella melitensis
3.4.11.2 additional information no or poor inhibition by leupeptin, PMSF, E64, and pepstatin Brucella melitensis
3.4.11.2 pefabloc 40% inhibition at 0.005 mM Brucella melitensis
3.4.11.2 Zn2+ complete inhibition at 1 mM Brucella melitensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.11.2 0.18
-
 Lys-4-nitroanilide pH 7.0, 37°C Brucella melitensis
3.4.11.2 0.35
-
 Ala-4-nitroanilide pH 7.0, 37°C Brucella melitensis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.11.2 additional information metallopeptidase, no or poor inhibition by Co2+ and Mg2+ Brucella melitensis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.11.2 96000
-
 1 * 96000, SDS-PAGE Brucella melitensis
3.4.11.2 97000
-
 gel filtration Brucella melitensis

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.2 Brucella melitensis Q8YG38 strain VTRM1, gene pepN
-
3.4.11.2 Brucella melitensis 16M Q8YG38 strain VTRM1, gene pepN
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.11.2 144fold Brucella melitensis

Storage Stability

EC Number Storage Stability Organism
3.4.11.2 -20°C, purified enzyme, 6 months, loss of 50% activity Brucella melitensis
3.4.11.2 -70°C, purified enzyme, 6 months, no loss of activity Brucella melitensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.11.2 Ala-4-nitroanilide + H2O
-
 Brucella melitensis Ala + 4-nitroaniline
-
 ?
3.4.11.2 Ala-4-nitroanilide + H2O
-
 Brucella melitensis 16M Ala + 4-nitroaniline
-
 ?
3.4.11.2 Lys-4-nitroanilide + H2O
-
 Brucella melitensis Lys + 4-nitroaniline
-
 ?
3.4.11.2 Lys-4-nitroanilide + H2O
-
 Brucella melitensis 16M Lys + 4-nitroaniline
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.11.2 monomer 1 * 96000, SDS-PAGE Brucella melitensis

Synonyms

EC Number Synonyms Comment Organism
3.4.11.2 aminopeptidase N
-
 Brucella melitensis
3.4.11.2 Ape
-
 Brucella melitensis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.11.2 40
-
-
 Brucella melitensis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.4.11.2 37 60 decreased activity above 50°C, 15% of maximal activity at 60°C Brucella melitensis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.11.2 50
-
 loss of 95% activity within 30 min Brucella melitensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.11.2 7
-
-
 Brucella melitensis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.11.2 6 9 no activity below pH 6.0 and above pH 9.0 Brucella melitensis

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.4.11.2 Brucella melitensis isoelectric chromatofocusing
-
 4.8