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Literature summary extracted from

  • Goldstein, J.M.; Nelson, D.; Kordula, T.; Mayo, J.A.; Travis, J.
    Extracellular arginine aminopeptidase from Streptococcus gordonii FSS2 (2002), Infect. Immun., 70, 836-843.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.11.6 2-mercaptoethanol 1 mM, activation to 160% of control Streptococcus gordonii
3.4.11.6 EDTA 5 mM, activation to 131% of control Streptococcus gordonii
3.4.11.6 L-Cys 5 mM, activation to 148% of control Streptococcus gordonii

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.11.6
-
 Streptococcus gordonii

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.11.6 3,4-dichloroisocoumarin 2 mM, 38% inhibition Streptococcus gordonii
3.4.11.6 amastatin 1 mM, 11% inhibition Streptococcus gordonii
3.4.11.6 apstatin 0.1 mM, 50% inhibition Streptococcus gordonii
3.4.11.6 bestatin 1 mM, 32% inhibition Streptococcus gordonii
3.4.11.6 DFP 5 mM, 25% inhibition. 10 mM, 44% inhibition Streptococcus gordonii
3.4.11.6 Glu-pyrrolidide 0.1 mM, 98% inhibition Streptococcus gordonii
3.4.11.6 Gly-Gly 5 mM, 8% inhibition Streptococcus gordonii
3.4.11.6 iodoacetamide 5 mM, 9% inhibition Streptococcus gordonii
3.4.11.6 L-Arg 5 mM, 70% inhibition Streptococcus gordonii
3.4.11.6 L-Lys 5 mM, 28% inhibition Streptococcus gordonii
3.4.11.6 PCMB 2 mM, complete inactivation Streptococcus gordonii
3.4.11.6 pefabloc 5 mM, 60% inhibition. 10 mM 69% inhibition Streptococcus gordonii
3.4.11.6 SDS 5%, 96% inhibition Streptococcus gordonii
3.4.11.6 Urea 2 M, 18% inhibition Streptococcus gordonii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.11.6 0.051
-
 Arg-4-nitroanilide pH 7.8, 37°C Streptococcus gordonii

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.11.6 extracellular
-
 Streptococcus gordonii
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.11.6 Ca2+ 5 mM, activation to 150% of control Streptococcus gordonii
3.4.11.6 K+ 5 mM, activation to 131% of control Streptococcus gordonii
3.4.11.6 Na+ 200 mM, activation to 166% of control Streptococcus gordonii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.11.6 64000
-
 gel filtration Streptococcus gordonii
3.4.11.6 70000
-
 1 * 70000, SDS-PAGE Streptococcus gordonii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.11.6 additional information Streptococcus gordonii the enzyme may serve as a critical factor for arginine acquisition during nutrient stress in vivo and also in the proteolysis of host proteins and peptides during SBE pathology ?
-
 ?
3.4.11.6 additional information Streptococcus gordonii FSS2 the enzyme may serve as a critical factor for arginine acquisition during nutrient stress in vivo and also in the proteolysis of host proteins and peptides during SBE pathology ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.6 Streptococcus gordonii Q938E9 FSS2
-
3.4.11.6 Streptococcus gordonii FSS2 Q938E9 FSS2
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.11.6
-
 Streptococcus gordonii

Storage Stability

EC Number Storage Stability Organism
3.4.11.6 -20°C, complete inactivation after 2 months Streptococcus gordonii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.11.6 Arg-4-nitroanilide + H2O
-
 Streptococcus gordonii Arg + 4-nitroaniline
-
 ?
3.4.11.6 Arg-Gly + H2O
-
 Streptococcus gordonii Arg + Gly
-
 ?
3.4.11.6 Arg-Gly-Asp-Ser-Pro-Ala-Ser-Ser-Lys-Pro + H2O i.e. fibronectin-binding inhibitor Streptococcus gordonii Arg + Gly-Asp-Ser-Pro-Ala-Ser-Ser-Lys-Pro
-
 ?
3.4.11.6 Arg-Gly-Glu-Ser + H2O i.e. platelet aggregation inhibitor Streptococcus gordonii Arg + Gly-Glu-Ser
-
 ?
3.4.11.6 Arg-Gly-Pro-Phe-Pro-Ile + H2O i.e. sexual agglutination peptide Streptococcus gordonii Arg + Gly-Pro-Phe-Pro-Ile
-
 ?
3.4.11.6 Arg-Phe + H2O
-
 Streptococcus gordonii Arg + Phe
-
 ?
3.4.11.6 Arg-Phe-Ala-Arg-Lys-Gly-Ala-Leu-Arg-Gln-Lys-Asn-Val + H2O i.e. protein kinase C substrate Streptococcus gordonii Arg + Phe-Ala-Arg-Lys-Gly-Ala-Leu-Arg-Gln-Lys-Asn-Val
-
 ?
3.4.11.6 Arg-Pro + H2O
-
 Streptococcus gordonii Arg + Pro
-
 ?
3.4.11.6 Arg-Pro + H2O
-
 Streptococcus gordonii FSS2 Arg + Pro
-
 ?
3.4.11.6 Arg-Pro-Lys-Pro-Gln-Gly-Leu-Met + H2O i.e. substance P Streptococcus gordonii Arg + Pro-Lys-Pro-Gln-Gly-Leu-Met
-
 ?
3.4.11.6 Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg + H2O i.e. bradykinin Streptococcus gordonii Arg + Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg
-
 ?
3.4.11.6 Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg + H2O i.e. bradykinin Streptococcus gordonii FSS2 Arg + Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg
-
 ?
3.4.11.6 Arg-Val-Tyr-Ile-His-Pro-Ile + H2O i.e. angiotensin III Streptococcus gordonii Arg + Val-Tyr-Ile-His-Pro-Ile
-
 ?
3.4.11.6 Leu-4-nitroanilide + H2O 7% of the activity with Arg-4-nitroanilide Streptococcus gordonii Leu + 4-nitroaniline
-
 ?
3.4.11.6 Leu-4-nitroanilide + H2O 7% of the activity with Arg-4-nitroanilide Streptococcus gordonii FSS2 Leu + 4-nitroaniline
-
 ?
3.4.11.6 Lys-4-nitroanilide + H2O 5% of the activity with Arg-4-nitroanilide Streptococcus gordonii Lys + 4-nitroaniline
-
 ?
3.4.11.6 additional information proteolysis is restricted to peptides with an arginine residue in the N terminus, with cleavage detected only when a hydrophobic or an uncharged residue occupies the second site Streptococcus gordonii ?
-
 ?
3.4.11.6 additional information the enzyme may serve as a critical factor for arginine acquisition during nutrient stress in vivo and also in the proteolysis of host proteins and peptides during SBE pathology Streptococcus gordonii ?
-
 ?
3.4.11.6 additional information proteolysis is restricted to peptides with an arginine residue in the N terminus, with cleavage detected only when a hydrophobic or an uncharged residue occupies the second site Streptococcus gordonii FSS2 ?
-
 ?
3.4.11.6 additional information the enzyme may serve as a critical factor for arginine acquisition during nutrient stress in vivo and also in the proteolysis of host proteins and peptides during SBE pathology Streptococcus gordonii FSS2 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.11.6 monomer 1 * 70000, SDS-PAGE Streptococcus gordonii

Synonyms

EC Number Synonyms Comment Organism
3.4.11.6 RAP
-
 Streptococcus gordonii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.11.6 4
-
 24 h, minimally affected Streptococcus gordonii
3.4.11.6 25
-
 unstable after 24 h Streptococcus gordonii
3.4.11.6 37
-
 unstable after 24 h Streptococcus gordonii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.11.6 6.5
-
-
 Streptococcus gordonii

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.4.11.6 Streptococcus gordonii isoelectric focusing 5.9 5.6