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Literature summary extracted from

  • Zagursky, R.J.; Ooi, P.; Jones, K.F.; Fiske, M.J.; Smith, R.P.; Green, B.A.
    Identification of a Haemophilus influenzae 5'-nucleotidase protein: cloning of the nucA gene and immunogenicity and characterization of the NucA protein (2000), Infect. Immun., 68, 2525-2534.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
3.1.3.5 medicine the enzyme is a potential subunit vaccine candidate against NTHi disease Haemophilus influenzae

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.5 expression in Escherichia coli, recombinant enzyme is localized in periplasm of Escherichia coli Haemophilus influenzae

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.5 Zn2+ 3.0 mM, 75% inhibition of recombinant enzyme, 20% inhibition of native enzyme Haemophilus influenzae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.3.5 additional information recombinant enzyme is localized in periplasm of Escherichia coli Haemophilus influenzae
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.5 Mg2+ 3.0 mM, maximal stimulation Haemophilus influenzae
3.1.3.5 Mn2+ 3.0 mM, stimulation Haemophilus influenzae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.5 63000
-
 x * 63000, SDS-PAGE Haemophilus influenzae
3.1.3.5 66200
-
 x * 66200, calculation from nucleotide sequence Haemophilus influenzae

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.5 Haemophilus influenzae
-
 NTHi strain P860295
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.3.5 additional information enzyme is not a lipoprotein Haemophilus influenzae

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.5
-
 Haemophilus influenzae

Subunits

EC Number Subunits Comment Organism
3.1.3.5 ? x * 63000, SDS-PAGE Haemophilus influenzae
3.1.3.5 ? x * 66200, calculation from nucleotide sequence Haemophilus influenzae