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Literature summary extracted from

  • Song, E.K.; Kim, H.; Sung, H.K.; Cha, J.
    Cloning and characterization of a levanbiohydrolase from Microbacterium laevaniformans ATCC 15953 (2002), Gene, 291, 45-55.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.64
-
 Microbacterium laevaniformans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.64 extracellular
-
 Microbacterium laevaniformans
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.64 68800
-
 x * 68800, SDS-PAGE Microbacterium laevaniformans

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.64 Microbacterium laevaniformans
-
 strain ATCC 15953
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.64 recombinant protein, expression n Escherichia coli Microbacterium laevaniformans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.64 997.3
-
 pH 6.0, 30°C Microbacterium laevaniformans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.64 1-kestose + H2O 0.2% of the activity with levan from Serratia sp. Microbacterium laevaniformans ?
-
 ?
3.2.1.64 inulin + H2O 5% of the activity with levan from Serratia sp. Microbacterium laevaniformans ?
-
 ?
3.2.1.64 levan + H2O enzyme activity depends on source of levan, sources in decreasing order of activity: Serratia sp., Rhanella aquatilis, Zymomonas mobilis, M. laevaniformans Microbacterium laevaniformans levanbiose + ?
-
 ?
3.2.1.64 additional information enzyme cleaves beta-2,6-linkage of levan and beta-2,1-linkage of fructooligosaccharides, no substrate are: soluble starch, cellobiose, sucrose, maltose, melibiose, raffinose, lactose Microbacterium laevaniformans ?
-
 ?
3.2.1.64 nystose + H2O 0.5% of the activity with levan from Serratia sp. Microbacterium laevaniformans ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.64 ? x * 68800, SDS-PAGE Microbacterium laevaniformans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.64 30
-
-
 Microbacterium laevaniformans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.64 40
-
 30 min, 10% loss of activity Microbacterium laevaniformans
3.2.1.64 50
-
 30 min, 65% loss of activiy Microbacterium laevaniformans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.64 6
-
-
 Microbacterium laevaniformans

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.64 5.5 7 more than 80% of maximum activity in this range Microbacterium laevaniformans