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Literature summary extracted from

  • Lee, S.W.; Jo, Y.J.; Kim, S.
    Cloning and characterization of mitochondrial methionyl-tRNA synthetase from a pathogenic fungi Candida albicans (1998), Gene, 215, 311-318.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.1.1.10 DNA and amino acid sequence determination and analysis, expression as His-tagged enzyme in Escherichia coli BL21(DE3), functional complementation of an enzyme-deficient Escherichia coli strain Candida albicans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
6.1.1.10 mitochondrion mitochondrial isozyme Candida albicans 5739
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.1.1.10 ATP + L-methionine + tRNAMet Candida albicans
-
 AMP + diphosphate + L-methionyl-tRNAMet
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.10 Candida albicans O74634 class I enzyme
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.1.1.10 recombinant His-tagged enzyme from Escherichia coli Candida albicans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.10 ATP + L-methionine + tRNAMet
-
 Candida albicans AMP + diphosphate + L-methionyl-tRNAMet
-
 ?
6.1.1.10 ATP + L-methionine + tRNAMet also utilizes both subtypes of Escherichia coli tRNAMet Candida albicans AMP + diphosphate + L-methionyl-tRNAMet
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.1.1.10 Methionine translase
-
 Candida albicans
6.1.1.10 Methionine--tRNA ligase
-
 Candida albicans
6.1.1.10 Methionyl tRNA synthetase
-
 Candida albicans
6.1.1.10 Methionyl-transfer ribonucleate synthetase
-
 Candida albicans
6.1.1.10 Methionyl-transfer ribonucleic acid synthetase
-
 Candida albicans
6.1.1.10 Methionyl-transfer RNA synthetase
-
 Candida albicans
6.1.1.10 MetRS
-
 Candida albicans
6.1.1.10 Synthetase, methionyl-transfer ribonucleate
-
 Candida albicans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.1.1.10 30
-
 assay at Candida albicans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.1.1.10 7.5
-
 assay at Candida albicans

Cofactor

EC Number Cofactor Comment Organism Structure
6.1.1.10 ATP
-
 Candida albicans