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Literature summary extracted from

  • Guimaraes, L.H.; Terenzi, H.F.; Jorge, J.A.; Leone, F.A.; Polizeli, M.L.
    Extracellular alkaline phosphatase from the filamentous fungus Aspergillus caespitosus: purification and biochemical characterization (2003), FEMS Microbiol. Rev., 48, 627-632.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.3.1 Mg2+ 95% activation at 1 mM Aspergillus caespitosus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.1 0.074
-
 4-nitrophenyl phosphate in absence of Mg2+ Aspergillus caespitosus
3.1.3.1 0.09
-
 4-nitrophenyl phosphate in presence of Mg2+ Aspergillus caespitosus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.3.1 extracellular
-
 Aspergillus caespitosus
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.1 57000
-
 2 * 57000, SDS-PAGE Aspergillus caespitosus
3.1.3.1 134800
-
 gel filtration Aspergillus caespitosus

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.1 Aspergillus caespitosus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.3.1 glycoprotein enzyme contains up to 56% carbohydrate Aspergillus caespitosus

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.1
-
 Aspergillus caespitosus

Reaction

EC Number Reaction Comment Organism Reaction ID
3.1.3.1 a phosphate monoester + H2O = an alcohol + phosphate Wide specificity. Also catalyses transphosphorylations. Some enzymes hydrolyse diphosphate Aspergillus caespitosus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.1 culture condition:xylan-grown cell
-
 Aspergillus caespitosus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.1 4-nitrophenyl phosphate + H2O
-
 Aspergillus caespitosus 4-nitrophenol + phosphate
-
 ?
3.1.3.1 O-phosphoserine + H2O
-
 Aspergillus caespitosus serine + phosphate
-
 ?
3.1.3.1 O-phosphothreonine + H2O
-
 Aspergillus caespitosus threonine + phosphate
-
 ?
3.1.3.1 O-phosphotyrosine + H2O
-
 Aspergillus caespitosus tyrosine + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.1 dimer 2 * 57000, SDS-PAGE Aspergillus caespitosus

Synonyms

EC Number Synonyms Comment Organism
3.1.3.1 alkaline phosphatase
-
 Aspergillus caespitosus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.1 75
-
-
 Aspergillus caespitosus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.3.1 50
-
 stable at Aspergillus caespitosus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.1 8.5
-
-
 Aspergillus caespitosus