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Literature summary extracted from

  • Chohnan, S.; Nonaka, J.; Teramoto, K.; Taniguchi, K.; Kameda, Y.; Tamura, H.; Kurusu, Y.; Norioka, S.; Masaki, T.; Sakiyama, F.
    Lysobacter strain with high lysyl endopeptidase production (2002), FEMS Microbiol. Lett., 213, 13-20.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.21.50
-
 Lysobacter sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.50 Ba2+ 1 mM, 24% inhibition of amidolytic activity Lysobacter sp.
3.4.21.50 diisopropyl fluorophosphate 10 mM, almost complete inhibition of amidolytic activity Lysobacter sp.
3.4.21.50 Nalpha-p-tosyl-L-Lys-chloromethylketone 0.1 mM, almost complete inhibition of amidolytic activity Lysobacter sp.
3.4.21.50 phenylmethanesulfonyl fluoride 2 mM, almost complete inhibition of amidolytic activity Lysobacter sp.
3.4.21.50 Zn2+ 1 mM, 34% inhibition of amidolytic activity Lysobacter sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.21.50 extracellular
-
 Lysobacter sp.
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.21.50 27000
-
 gel filtration Lysobacter sp.
3.4.21.50 27850
-
 ion-spray mass spectrometry Lysobacter sp.
3.4.21.50 31000
-
 1 * 31000, SDS-PAGE Lysobacter sp.

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.50 Lysobacter sp. Q9AQR5
-
-
3.4.21.50 Lysobacter sp. IB-9374 Q9AQR5
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.21.50 proteolytic modification the total precursor protein is 671 amino acids of which only 268 amino acids are in the finally processed exoenzyme Lysobacter sp.

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.50
-
 Lysobacter sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.21.50 culture medium
-
 Lysobacter sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.21.50 5.6
-
-
 Lysobacter sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.50 acetyl-Lys-p-nitroaniline + H2O
-
 Lysobacter sp. acetyl-Lys + p-nitroaniline
-
 ?
3.4.21.50 amyloid beta-protein + H2O only the Lys-X bond is hydrolyzed Lysobacter sp. ?
-
 ?
3.4.21.50 amyloid beta-protein + H2O only the Lys-X bond is hydrolyzed Lysobacter sp. IB-9374 ?
-
 ?
3.4.21.50 benzoyl-Lys methyl ester + H2O
-
 Lysobacter sp. benzoyl-Lys + methanol
-
 ?
3.4.21.50 benzoyl-Lys methyl ester + H2O
-
 Lysobacter sp. IB-9374 benzoyl-Lys + methanol
-
 ?
3.4.21.50 benzoyl-Lys-p-nitroanilide + H2O
-
 Lysobacter sp. benzoyl-Lys + p-nitroaniline
-
 ?
3.4.21.50 benzoyl-Lys-p-nitroaniline + H2O
-
 Lysobacter sp. benzoyl-Lys + p-nitroaniline
-
 ?
3.4.21.50 benzoyl-Orn methyl ester + H2O
-
 Lysobacter sp. benzoyl-Orn + methanol
-
 ?
3.4.21.50 Glucagon + H2O only the Lys-X bond is hydrolyzed Lysobacter sp. ?
-
 ?
3.4.21.50 Glucagon + H2O only the Lys-X bond is hydrolyzed Lysobacter sp. IB-9374 ?
-
 ?
3.4.21.50 additional information no activity with benzoyl-Arg-p-nitroanilide, Arg-p-nitroanilide, succinyl-Ala-p-nitroanilide, tert-butyloxycarbonyl-His-p-nitroanilide, acetyl-Phe-p-nitroanilide, benzoyl-Tyr-p-nitroanilide and acetyl-Leu-p-nitroanilide Lysobacter sp. ?
-
 ?
3.4.21.50 additional information no activity with benzoyl-Arg-p-nitroanilide, Arg-p-nitroanilide, succinyl-Ala-p-nitroanilide, tert-butyloxycarbonyl-His-p-nitroanilide, acetyl-Phe-p-nitroanilide, benzoyl-Tyr-p-nitroanilide and acetyl-Leu-p-nitroanilide Lysobacter sp. IB-9374 ?
-
 ?
3.4.21.50 oxidized insulin B chain + H2O only the Lys-X bond is hydrolyzed Lysobacter sp. ?
-
 ?
3.4.21.50 oxidized insulin B chain + H2O only the Lys-X bond is hydrolyzed Lysobacter sp. IB-9374 ?
-
 ?
3.4.21.50 tosyl-Lys methyl ester + H2O
-
 Lysobacter sp. tosyl-Lys + methanol
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.21.50 monomer 1 * 31000, SDS-PAGE Lysobacter sp.

Synonyms

EC Number Synonyms Comment Organism
3.4.21.50 LEP
-
 Lysobacter sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.21.50 50
-
 hydrolysis of benzoyl-Lys-p-nitroanilide Lysobacter sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.21.50 45
-
 pH 8.0, 30°C, stable Lysobacter sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.21.50 8 8.5 esterolytic activity Lysobacter sp.
3.4.21.50 8.5 10.5 caseinolytic activity Lysobacter sp.
3.4.21.50 9 9.6 amidolytic activity Lysobacter sp.

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.4.21.50 Lysobacter sp. isoelectric focusing
-
 6.8