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Literature summary extracted from

  • Kim, B.C.; Lee, Y.H.; Lee, H.S.; Lee, D.W.; Choe, E.A.; Pyun, Y.R.
    Cloning, expression and characterization of L-arabinose isomerase from Thermotoga neapolitana: bioconversion of D-galactose to D-tagatose using the enzyme (2002), FEMS Microbiol. Lett., 212, 121-126.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.3.1.4 expression in Escherichia coli Thermotoga neapolitana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.3.1.4 116
-
 L-arabinose pH 7.0, 85°C Thermotoga neapolitana
5.3.1.4 250
-
 D-galactose pH 7.0, 85°C Thermotoga neapolitana

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.3.1.4 Co2+ divalent cation, maximal activity in presence of both Mn2+ and Co2+ Thermotoga neapolitana
5.3.1.4 Mn2+ divalent cation, maximal activity in presence of both Mn2+ and Co2+ Thermotoga neapolitana

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.3.1.4 56677
-
 x * 56677, calculation from nucleotide sequence Thermotoga neapolitana

Organism

EC Number Organism UniProt Comment Textmining
5.3.1.4 Thermotoga neapolitana
-
 5068
-
5.3.1.4 Thermotoga neapolitana 5068
-
 5068
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.3.1.4 expression in Escherichia coli Thermotoga neapolitana

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.3.1.4 additional information
-
-
 Thermotoga neapolitana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.1.4 D-galactose the conversion equilibrium shifts to more ketone sugar from aldose sugar as the isomerization reaction temperature raises. 68% conversion of D-galactose to D-tagatose at 80°C Thermotoga neapolitana D-tagatose
-
 r
5.3.1.4 D-galactose the conversion equilibrium shifts to more ketone sugar from aldose sugar as the isomerization reaction temperature raises. 68% conversion of D-galactose to D-tagatose at 80°C Thermotoga neapolitana 5068 D-tagatose
-
 r
5.3.1.4 L-arabinose
-
 Thermotoga neapolitana L-ribulose
-
 ?
5.3.1.4 L-arabinose
-
 Thermotoga neapolitana 5068 L-ribulose
-
 ?

Subunits

EC Number Subunits Comment Organism
5.3.1.4 ? x * 56677, calculation from nucleotide sequence Thermotoga neapolitana

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.3.1.4 85
-
 L-arabinose and D-galactose isomerization Thermotoga neapolitana

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
5.3.1.4 80
-
 2 h, in absence of metal ions more than 50% loss of activity, in presence of Co2+ and Mn2+ the enzyme retains 80% of its original activity Thermotoga neapolitana
5.3.1.4 90
-
 half-life without metal ions: 70 min, half-life with 1 mM CoCl2 and 1 mM MnCl2: 120 min Thermotoga neapolitana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.3.1.4 7
-
-
 Thermotoga neapolitana