BRENDA - Enzyme Database

Molecular characterization of phosphoglycerate mutase in archaea

van der Oost, J.; Huynen, M.A.; Verhees, C.H.; FEMS Microbiol. Lett. 212, 111-120 (2002)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
5.4.2.12
expression in Escherichia coli
Methanocaldococcus jannaschii
5.4.2.12
expression in Escherichia coli
Pyrococcus furiosus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
5.4.2.12
EDTA
2 mM, 20% inhibition
Methanocaldococcus jannaschii
5.4.2.12
EDTA
2 mM, 20% inhibition
Pyrococcus furiosus
5.4.2.12
Zn2+
10 mM, 81% inhibition
Methanocaldococcus jannaschii
5.4.2.12
Zn2+
10 mM, 85% inhibition
Pyrococcus furiosus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
5.4.2.12
0.33
-
3-phosphoglycerate
50C, pH 8.0
Methanocaldococcus jannaschii
5.4.2.12
0.49
-
3-phospho-D-glycerate
50C, pH 8.0
Pyrococcus furiosus
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
5.4.2.12
Mg2+
10 mM, slight activation
Methanocaldococcus jannaschii
5.4.2.12
Mg2+
10 mM, slight activation
Pyrococcus furiosus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
5.4.2.12
45200
-
4 * 45200, deduced from nucleotide sequence
Methanocaldococcus jannaschii
5.4.2.12
45300
-
4 * 45300, deduced from nucleotide sequence
Pyrococcus furiosus
5.4.2.12
183500
-
gel filtration
Pyrococcus furiosus
5.4.2.12
190600
-
gel filtration
Methanocaldococcus jannaschii
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
5.4.2.12
2-phospho-D-glycerate
Pyrococcus furiosus
-
3-phospho-D-glycerate
-
Pyrococcus furiosus
r
5.4.2.12
2-phospho-D-glycerate
Methanocaldococcus jannaschii
-
3-phospho-D-glycerate
-
Methanocaldococcus jannaschii
r
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
5.4.2.12
Methanocaldococcus jannaschii
-
-
-
5.4.2.12
Pyrococcus furiosus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
5.4.2.12
recombinant iPGM
Methanocaldococcus jannaschii
5.4.2.12
recombinant iPGM
Pyrococcus furiosus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.4.2.12
2-phospho-D-glycerate
-
651372
Pyrococcus furiosus
3-phospho-D-glycerate
-
651372
Pyrococcus furiosus
r
5.4.2.12
2-phospho-D-glycerate
-
651372
Methanocaldococcus jannaschii
3-phospho-D-glycerate
-
651372
Methanocaldococcus jannaschii
r
Subunits
EC Number
Subunits
Commentary
Organism
5.4.2.12
tetramer
4 * 45200, deduced from nucleotide sequence
Methanocaldococcus jannaschii
5.4.2.12
tetramer
4 * 45300, deduced from nucleotide sequence
Pyrococcus furiosus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.4.2.12
8
-
-
Methanocaldococcus jannaschii
5.4.2.12
8
-
-
Pyrococcus furiosus
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
5.4.2.12
additional information
2,3-bisphosphoglycerate independent phosphoglycerate mutase
Methanocaldococcus jannaschii
5.4.2.12
additional information
2,3-bisphosphoglycerate independent phosphoglycerate mutase
Pyrococcus furiosus
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
5.4.2.12
expression in Escherichia coli
Methanocaldococcus jannaschii
5.4.2.12
expression in Escherichia coli
Pyrococcus furiosus
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
5.4.2.12
additional information
2,3-bisphosphoglycerate independent phosphoglycerate mutase
Methanocaldococcus jannaschii
5.4.2.12
additional information
2,3-bisphosphoglycerate independent phosphoglycerate mutase
Pyrococcus furiosus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
5.4.2.12
EDTA
2 mM, 20% inhibition
Methanocaldococcus jannaschii
5.4.2.12
EDTA
2 mM, 20% inhibition
Pyrococcus furiosus
5.4.2.12
Zn2+
10 mM, 81% inhibition
Methanocaldococcus jannaschii
5.4.2.12
Zn2+
10 mM, 85% inhibition
Pyrococcus furiosus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
5.4.2.12
0.33
-
3-phosphoglycerate
50C, pH 8.0
Methanocaldococcus jannaschii
5.4.2.12
0.49
-
3-phospho-D-glycerate
50C, pH 8.0
Pyrococcus furiosus
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
5.4.2.12
Mg2+
10 mM, slight activation
Methanocaldococcus jannaschii
5.4.2.12
Mg2+
10 mM, slight activation
Pyrococcus furiosus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
5.4.2.12
45200
-
4 * 45200, deduced from nucleotide sequence
Methanocaldococcus jannaschii
5.4.2.12
45300
-
4 * 45300, deduced from nucleotide sequence
Pyrococcus furiosus
5.4.2.12
183500
-
gel filtration
Pyrococcus furiosus
5.4.2.12
190600
-
gel filtration
Methanocaldococcus jannaschii
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
5.4.2.12
2-phospho-D-glycerate
Pyrococcus furiosus
-
3-phospho-D-glycerate
-
Pyrococcus furiosus
r
5.4.2.12
2-phospho-D-glycerate
Methanocaldococcus jannaschii
-
3-phospho-D-glycerate
-
Methanocaldococcus jannaschii
r
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
5.4.2.12
recombinant iPGM
Methanocaldococcus jannaschii
5.4.2.12
recombinant iPGM
Pyrococcus furiosus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.4.2.12
2-phospho-D-glycerate
-
651372
Pyrococcus furiosus
3-phospho-D-glycerate
-
651372
Pyrococcus furiosus
r
5.4.2.12
2-phospho-D-glycerate
-
651372
Methanocaldococcus jannaschii
3-phospho-D-glycerate
-
651372
Methanocaldococcus jannaschii
r
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
5.4.2.12
tetramer
4 * 45200, deduced from nucleotide sequence
Methanocaldococcus jannaschii
5.4.2.12
tetramer
4 * 45300, deduced from nucleotide sequence
Pyrococcus furiosus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.4.2.12
8
-
-
Methanocaldococcus jannaschii
5.4.2.12
8
-
-
Pyrococcus furiosus