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Literature summary extracted from

  • Lucas, P.; Lonvaud-Funel, A.
    Purification and partial gene sequence of the tyrosine decarboxylase of Lactobacillus brevis IOEB 9809 (2002), FEMS Microbiol. Lett., 211, 85-89.
    View publication on PubMed

General Stability

EC Number General Stability Organism
4.1.1.25 enzyme is stable during preparation, pyridoxal 5’-phosphate, glycerol, EDTA and 2-mercaptoethanol stabilize Levilactobacillus brevis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.1.1.25 soluble in the soluble fraction Levilactobacillus brevis
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.25 70000
-
 2 * 70000, SDS-PAGE Levilactobacillus brevis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.1.25 L-Tyrosine Levilactobacillus brevis
-
 Tyramine + CO2
-
 ?
4.1.1.25 L-Tyrosine Levilactobacillus brevis IOEB 9809
-
 Tyramine + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.25 Levilactobacillus brevis Q7B9Y8
-
-
4.1.1.25 Levilactobacillus brevis IOEB 9809 Q7B9Y8
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.25 67.6fold Levilactobacillus brevis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.1.25 additional information
-
 not detectable in crude extract, but in the soluble fraction Levilactobacillus brevis
4.1.1.25 0.00000243
-
 pH 5, 37°C, purified TDC Levilactobacillus brevis

Storage Stability

EC Number Storage Stability Organism
4.1.1.25 -20°C, stable Levilactobacillus brevis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.25 L-Tyrosine
-
 Levilactobacillus brevis Tyramine + CO2
-
 ?
4.1.1.25 L-Tyrosine specific for tyrosine Levilactobacillus brevis Tyramine + CO2
-
 ?
4.1.1.25 L-Tyrosine
-
 Levilactobacillus brevis IOEB 9809 Tyramine + CO2
-
 ?
4.1.1.25 L-Tyrosine specific for tyrosine Levilactobacillus brevis IOEB 9809 Tyramine + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.1.25 homodimer 2 * 70000, SDS-PAGE Levilactobacillus brevis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.1.25 37
-
 assay at Levilactobacillus brevis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.1.25 5
-
 assay at Levilactobacillus brevis

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.1.25 pyridoxal 5'-phosphate requirement Levilactobacillus brevis