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Literature summary extracted from

  • Strych, U.; Penland, R.L.; Jimenez, M.; Krause, K.L.; Benedik, M.J.
    Characterization of the alanine racemases from two mycobacteria (2001), FEMS Microbiol. Lett., 196, 93-98.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.1.1.1 amplified by PCR, cloned and overexpressed in Escherichia coli BL21, both as native and His-tagged products Mycobacterium avium
5.1.1.1 amplified by PCR, cloned and overexpressed in Escherichia coli BL21, both as native and His-tagged products Mycobacterium tuberculosis

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.1.1.1 cycloserine D-cycloserine or a racemic mixture of D- and L-cycloserine Mycobacterium avium
5.1.1.1 cycloserine D-cycloserine or a racemic mixture of D- and L-cycloserine Mycobacterium tuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.1.1.1 0.4
-
 D-Ala
-
 Mycobacterium avium
5.1.1.1 0.5
-
 L-Ala
-
 Mycobacterium avium
5.1.1.1 1.1
-
 D-Ala 23°C Mycobacterium tuberculosis
5.1.1.1 1.2
-
 L-Ala 23°C Mycobacterium tuberculosis

Organism

EC Number Organism UniProt Comment Textmining
5.1.1.1 Mycobacterium avium
-
-
-
5.1.1.1 Mycobacterium tuberculosis P9WQA9
-
-
5.1.1.1 Mycobacterium tuberculosis H37Rv P9WQA9
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.1.1.1
-
 Mycobacterium avium
5.1.1.1
-
 Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.1.1.1 L-Ala
-
 Mycobacterium avium D-Ala
-
 r
5.1.1.1 L-Ala
-
 Mycobacterium tuberculosis D-Ala
-
 r
5.1.1.1 L-Ala
-
 Mycobacterium tuberculosis H37Rv D-Ala
-
 r