BRENDA - Enzyme Database

In vivo formation of glutamyl-tRNA(Gln) in Escherichia coli by heterologous glutamyl-tRNA synthetases

Nunez, H.; Lefimil, C.; Min, B.; Soll, D.; Orellana, O.; FEBS Lett. 557, 133-135 (2004)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
6.1.1.17
expression in Escherichia coli; gene gltX1 and gltX2, expression in Escherichia coli temperature-sensitive mutant strain
Acidithiobacillus ferrooxidans
6.1.1.17
gene gltX mutant, mutant enzyme expression in Escherichia coli temperature-sensitive mutant strain
Bacillus subtilis
6.1.1.17
gene gltX2, expression in Escherichia coli temperature-sensitive mutant strain
Helicobacter pylori
6.1.1.24
expression in Escherichia coli
Acidithiobacillus ferrooxidans
6.1.1.24
expression in Escherichia coli
Helicobacter pylori
Engineering
EC Number
Amino acid exchange
Commentary
Organism
6.1.1.17
Q373R
substrate specificity restricted to tRNAGlu compared to the wild-type which also accepts tRNAGln
Bacillus subtilis
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
6.1.1.17
ATP + L-glutamate + tRNAGln
Helicobacter pylori
enzyme expressed in an Escherichia coli mutant strain, tRNAGln UUG from Escherichia coli
AMP + diphosphate + L-glutamyl-tRNAGln
-
Helicobacter pylori
?
6.1.1.17
ATP + L-glutamate + tRNAGln
Acidithiobacillus ferrooxidans
isozyme 2 expressed in an Escherichia coli mutant strain, tRNAGln UUG from Escherichia coli
AMP + diphosphate + L-glutamyl-tRNAGln
-
Acidithiobacillus ferrooxidans
?
6.1.1.17
ATP + L-glutamate + tRNAGlu
Helicobacter pylori
-
AMP + diphosphate + L-glutamyl-tRNAGlu
-
Helicobacter pylori
?
6.1.1.17
ATP + L-glutamate + tRNAGlu
Acidithiobacillus ferrooxidans
isozyme 1 expressed in an Escherichia coli mutant strain, tRNAGlu from Escherichia coli
AMP + diphosphate + L-glutamyl-tRNAGlu
-
Acidithiobacillus ferrooxidans
?
6.1.1.17
ATP + L-glutamate + tRNAGlu
Bacillus subtilis
recombinant mutant Q373R expressed in Escherichia coli mutant strain, tRNAGlu from Escherichia coli
AMP + diphosphate + L-glutamyl-tRNAGlu
-
Bacillus subtilis
?
6.1.1.17
additional information
Bacillus subtilis
no charging of Escherichia coli tRNAGln by enzyme mutant Q373R
?
-
Bacillus subtilis
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
6.1.1.17
Acidithiobacillus ferrooxidans
-
; strain ATCC 23270, isoform 1 and 2, genes gltX1 and gltX2
-
6.1.1.17
Bacillus subtilis
-
-
-
6.1.1.17
Helicobacter pylori
-
isozyme 2
-
6.1.1.24
Acidithiobacillus ferrooxidans
-
-
-
6.1.1.24
Helicobacter pylori
-
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6.1.1.17
ATP + Glu + tRNAGln
-
651337
Acidithiobacillus ferrooxidans
AMP + diphosphate + L-glutamyl-tRNAGln
-
-
-
?
6.1.1.17
ATP + L-glutamate + tRNAGln
enzyme expressed in an Escherichia coli mutant strain, tRNAGln UUG from Escherichia coli
651337
Helicobacter pylori
AMP + diphosphate + L-glutamyl-tRNAGln
-
651337
Helicobacter pylori
?
6.1.1.17
ATP + L-glutamate + tRNAGln
isozyme 2 expressed in an Escherichia coli mutant strain, tRNAGln UUG from Escherichia coli
651337
Acidithiobacillus ferrooxidans
AMP + diphosphate + L-glutamyl-tRNAGln
-
651337
Acidithiobacillus ferrooxidans
?
6.1.1.17
ATP + L-glutamate + tRNAGlu
-
651337
Helicobacter pylori
AMP + diphosphate + L-glutamyl-tRNAGlu
-
651337
Helicobacter pylori
?
6.1.1.17
ATP + L-glutamate + tRNAGlu
isozyme 1 expressed in an Escherichia coli mutant strain, tRNAGlu from Escherichia coli
651337
Acidithiobacillus ferrooxidans
AMP + diphosphate + L-glutamyl-tRNAGlu
-
651337
Acidithiobacillus ferrooxidans
?
6.1.1.17
ATP + L-glutamate + tRNAGlu
recombinant mutant Q373R expressed in Escherichia coli mutant strain, tRNAGlu from Escherichia coli
651337
Bacillus subtilis
AMP + diphosphate + L-glutamyl-tRNAGlu
-
651337
Bacillus subtilis
?
6.1.1.17
additional information
no charging of Escherichia coli tRNAGln by enzyme mutant Q373R
651337
Bacillus subtilis
?
-
651337
Bacillus subtilis
?
6.1.1.24
ATP + Glu + tRNAGln
-
651337
Acidithiobacillus ferrooxidans
AMP + diphosphate + L-glutamyl-tRNAGln
-
-
-
?
6.1.1.24
ATP + Glu + tRNAGln
-
651337
Helicobacter pylori
AMP + diphosphate + L-glutamyl-tRNAGln
-
-
-
?
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
6.1.1.17
ATP
-
Bacillus subtilis
6.1.1.17
ATP
-
Acidithiobacillus ferrooxidans
6.1.1.17
ATP
-
Helicobacter pylori
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
6.1.1.17
expression in Escherichia coli; gene gltX1 and gltX2, expression in Escherichia coli temperature-sensitive mutant strain
Acidithiobacillus ferrooxidans
6.1.1.17
gene gltX mutant, mutant enzyme expression in Escherichia coli temperature-sensitive mutant strain
Bacillus subtilis
6.1.1.17
gene gltX2, expression in Escherichia coli temperature-sensitive mutant strain
Helicobacter pylori
6.1.1.24
expression in Escherichia coli
Acidithiobacillus ferrooxidans
6.1.1.24
expression in Escherichia coli
Helicobacter pylori
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
6.1.1.17
ATP
-
Bacillus subtilis
6.1.1.17
ATP
-
Acidithiobacillus ferrooxidans
6.1.1.17
ATP
-
Helicobacter pylori
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
6.1.1.17
Q373R
substrate specificity restricted to tRNAGlu compared to the wild-type which also accepts tRNAGln
Bacillus subtilis
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
6.1.1.17
ATP + L-glutamate + tRNAGln
Helicobacter pylori
enzyme expressed in an Escherichia coli mutant strain, tRNAGln UUG from Escherichia coli
AMP + diphosphate + L-glutamyl-tRNAGln
-
Helicobacter pylori
?
6.1.1.17
ATP + L-glutamate + tRNAGln
Acidithiobacillus ferrooxidans
isozyme 2 expressed in an Escherichia coli mutant strain, tRNAGln UUG from Escherichia coli
AMP + diphosphate + L-glutamyl-tRNAGln
-
Acidithiobacillus ferrooxidans
?
6.1.1.17
ATP + L-glutamate + tRNAGlu
Helicobacter pylori
-
AMP + diphosphate + L-glutamyl-tRNAGlu
-
Helicobacter pylori
?
6.1.1.17
ATP + L-glutamate + tRNAGlu
Acidithiobacillus ferrooxidans
isozyme 1 expressed in an Escherichia coli mutant strain, tRNAGlu from Escherichia coli
AMP + diphosphate + L-glutamyl-tRNAGlu
-
Acidithiobacillus ferrooxidans
?
6.1.1.17
ATP + L-glutamate + tRNAGlu
Bacillus subtilis
recombinant mutant Q373R expressed in Escherichia coli mutant strain, tRNAGlu from Escherichia coli
AMP + diphosphate + L-glutamyl-tRNAGlu
-
Bacillus subtilis
?
6.1.1.17
additional information
Bacillus subtilis
no charging of Escherichia coli tRNAGln by enzyme mutant Q373R
?
-
Bacillus subtilis
?
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6.1.1.17
ATP + Glu + tRNAGln
-
651337
Acidithiobacillus ferrooxidans
AMP + diphosphate + L-glutamyl-tRNAGln
-
-
-
?
6.1.1.17
ATP + L-glutamate + tRNAGln
enzyme expressed in an Escherichia coli mutant strain, tRNAGln UUG from Escherichia coli
651337
Helicobacter pylori
AMP + diphosphate + L-glutamyl-tRNAGln
-
651337
Helicobacter pylori
?
6.1.1.17
ATP + L-glutamate + tRNAGln
isozyme 2 expressed in an Escherichia coli mutant strain, tRNAGln UUG from Escherichia coli
651337
Acidithiobacillus ferrooxidans
AMP + diphosphate + L-glutamyl-tRNAGln
-
651337
Acidithiobacillus ferrooxidans
?
6.1.1.17
ATP + L-glutamate + tRNAGlu
-
651337
Helicobacter pylori
AMP + diphosphate + L-glutamyl-tRNAGlu
-
651337
Helicobacter pylori
?
6.1.1.17
ATP + L-glutamate + tRNAGlu
isozyme 1 expressed in an Escherichia coli mutant strain, tRNAGlu from Escherichia coli
651337
Acidithiobacillus ferrooxidans
AMP + diphosphate + L-glutamyl-tRNAGlu
-
651337
Acidithiobacillus ferrooxidans
?
6.1.1.17
ATP + L-glutamate + tRNAGlu
recombinant mutant Q373R expressed in Escherichia coli mutant strain, tRNAGlu from Escherichia coli
651337
Bacillus subtilis
AMP + diphosphate + L-glutamyl-tRNAGlu
-
651337
Bacillus subtilis
?
6.1.1.17
additional information
no charging of Escherichia coli tRNAGln by enzyme mutant Q373R
651337
Bacillus subtilis
?
-
651337
Bacillus subtilis
?
6.1.1.24
ATP + Glu + tRNAGln
-
651337
Acidithiobacillus ferrooxidans
AMP + diphosphate + L-glutamyl-tRNAGln
-
-
-
?
6.1.1.24
ATP + Glu + tRNAGln
-
651337
Helicobacter pylori
AMP + diphosphate + L-glutamyl-tRNAGln
-
-
-
?