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Literature summary extracted from

  • Iozef, R.; Rahlfs, S.; Chang, T.; Schirmer, H.; Becker, K.
    Glyoxalase I of the malarial parasite Plasmodium falciparum: evidence for subunit fusion (2003), FEBS Lett., 554, 284-288.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.4.1.5 expression in Escherichia coli Plasmodium falciparum

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.4.1.5 methylglutathione
-
 Plasmodium falciparum
4.4.1.5 S-nitrosoglutathione
-
 Plasmodium falciparum
4.4.1.5 S-p-nitrosobenzylglutathione
-
 Plasmodium falciparum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.4.1.5 0.077
-
 glutathione-methylglyoxal hemithioacetal
-
 Plasmodium falciparum
4.4.1.5 0.58
-
 glutathione-glyoxal hemithioacetal
-
 Plasmodium falciparum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.4.1.5 Ni2+ 2.9fold increase in activity at 0.25 mM Plasmodium falciparum
4.4.1.5 Zn2+ 2.7fold increase in activity at 0.25 mM Plasmodium falciparum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.4.1.5 42300
-
 gel filtration Plasmodium falciparum
4.4.1.5 43600
-
 1 * 43600, SDS-PAGE, recombinant His-tagged PfGlx I Plasmodium falciparum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.4.1.5 glutathione-glyoxal hemithioacetal Plasmodium falciparum
-
 ?
-
 ?
4.4.1.5 glutathione-methylglyoxal hemithioacetal Plasmodium falciparum glutathione-methylglyoxal hemithioacetal is formed non-enzymatically from methylglyoxal and glutathione (R)-S-lactoylglutathione
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.4.1.5 Plasmodium falciparum Q71KM3
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.4.1.5 90
-
-
 Plasmodium falciparum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.4.1.5 glutathione-glyoxal hemithioacetal
-
 Plasmodium falciparum ?
-
 ?
4.4.1.5 glutathione-methylglyoxal hemithioacetal glutathione-methylglyoxal hemithioacetal is formed non-enzymatically from methylglyoxal and glutathione Plasmodium falciparum (R)-S-lactoylglutathione
-
 ?

Subunits

EC Number Subunits Comment Organism
4.4.1.5 monomer 1 * 43600, SDS-PAGE, recombinant His-tagged PfGlx I Plasmodium falciparum

Synonyms

EC Number Synonyms Comment Organism
4.4.1.5 glyoxalase I
-
 Plasmodium falciparum
4.4.1.5 PfGlx I
-
 Plasmodium falciparum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.4.1.5 66.7
-
 glutathione-methylglyoxal hemithioacetal
-
 Plasmodium falciparum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.4.1.5 7.8
-
-
 Plasmodium falciparum

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.4.1.5 0.06
-
 S-p-nitrosobenzylglutathione
-
 Plasmodium falciparum
4.4.1.5 0.17
-
 methylglutathione
-
 Plasmodium falciparum
4.4.1.5 0.19
-
 S-p-nitrosobenzylglutathione
-
 Plasmodium falciparum