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Literature summary extracted from

  • Zhang, D.; Li, N.; Swaminathan, K.; Zhang, L.H.
    A motif rich in charged residues determines product specificity in isomaltulose synthase (2003), FEBS Lett., 534, 151-155.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
5.4.99.11 D327N turnover number for sucrose: 1.4fold decrease, KM-value for sucrose: 1.65fold increase, alpha-D-glucopyranosyl-1,6-D-fructofuranose content: 43.7% decrease, alpha-D-glucopyranosyl-1,1-D-fructofuranose content: 41.0% increase Klebsiella sp.
5.4.99.11 D327R turnover number for sucrose: 2.45fold decrease, KM-value for sucrose: 2.2fold increase, alpha-D-glucopyranosyl-1,6-D-fructofuranose content: 26.5% decrease, alpha-D-glucopyranosyl-1,1-D-fructofuranose content: 17.7% increase Klebsiella sp.
5.4.99.11 D329N turnover number for sucrose: 1.17fold decrease, KM-value for sucrose: 2.9fold increase, alpha-D-glucopyranosyl-1,6-D-fructofuranose content: 52.4% decrease, alpha-D-glucopyranosyl-1,1-D-fructofuranose content: 54.7% increase Klebsiella sp.
5.4.99.11 R325D turnover number for sucrose: 14.5fold decrease, KM-value for sucrose: 1.2fold decrease, alpha-D-glucopyranosyl-1,6-D-fructofuranose content: 67% decrease, alpha-D-glucopyranosyl-1,1-D-fructofuranose content: 43.6% increase Klebsiella sp.
5.4.99.11 R325L turnover number for sucrose: 14.5fold decrease, KM-value for sucrose: 1.1fold decrease, alpha-D-glucopyranosyl-1,6-D-fructofuranose content: 54.3% decrease, alpha-D-glucopyranosyl-1,1-D-fructofuranose content: 31.3% increase Klebsiella sp.
5.4.99.11 R328D turnover number for sucrose: 11.9fold decrease, KM-value for sucrose: 2fold increase, alpha-D-glucopyranosyl-1,6-D-fructofuranose content: 65.6% decrease, alpha-D-glucopyranosyl-1,1-D-fructofuranose content: 61.2% increase Klebsiella sp.
5.4.99.11 R328L turnover number for sucrose: 14.5fold decrease, KM-value for sucrose: 5fold increase, alpha-D-glucopyranosyl-1,6-D-fructofuranose content: 51.9% decrease, alpha-D-glucopyranosyl-1,1-D-fructofuranose content: 50.3% increase Klebsiella sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.4.99.11 44
-
 sucrose mutant enzyme R325D, pH 6.0, 35°C Klebsiella sp.
5.4.99.11 49.1
-
 sucrose mutant enzyme R325L, pH 6.0, 35°C Klebsiella sp.
5.4.99.11 54.3
-
 sucrose wild-type enzyme, pH 6.0, 35°C Klebsiella sp.
5.4.99.11 89.9
-
 sucrose mutant enzyme D327N, pH 6.0, 35°C Klebsiella sp.
5.4.99.11 108.9
-
 sucrose mutant enzyme R328D, pH 6.0, 35°C Klebsiella sp.
5.4.99.11 121.7
-
 sucrose mutant enzyme D327R, pH 6.0, 35°C Klebsiella sp.
5.4.99.11 157.7
-
 sucrose mutant enzyme R329N, pH 6.0, 35°C Klebsiella sp.
5.4.99.11 195.5
-
 sucrose mutant enzyme R328L, pH 6.0, 35°C Klebsiella sp.

Organism

EC Number Organism UniProt Comment Textmining
5.4.99.11 Klebsiella sp.
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.4.99.11 sucrose
-
 Klebsiella sp. alpha-D-glucopyranosyl-1,6-D-fructofuranose + alpha-D-glucopyranosyl-1,1-D-fructofuranose wild-type enzyme: 82.8% alpha-D-glucopyranosyl-1,6-D-fructofuranose and 12.1% alpha-D-glucopyranosyl-1,1-D-fructofuranose ?

Synonyms

EC Number Synonyms Comment Organism
5.4.99.11 isomaltulose synthase
-
 Klebsiella sp.
5.4.99.11 PalI
-
 Klebsiella sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.4.99.11 720
-
 sucrose mutant enzyme R325D and R325L, pH 6.0, 35°C Klebsiella sp.
5.4.99.11 876
-
 sucrose mutant enzyme R328D, pH 6.0, 35°C Klebsiella sp.
5.4.99.11 2070
-
 sucrose mutant enzyme R328L, pH 6.0, 35°C Klebsiella sp.
5.4.99.11 4260
-
 sucrose mutant enzyme D327R, pH 6.0, 35°C Klebsiella sp.
5.4.99.11 7368
-
 sucrose mutant enzyme D327N, pH 6.0, 35°C Klebsiella sp.
5.4.99.11 7370
-
 sucrose mutant enzyme D327N, pH 6.0, 35°C Klebsiella sp.
5.4.99.11 8898
-
 sucrose mutant enzyme D329L, pH 6.0, 35°C Klebsiella sp.
5.4.99.11 8900
-
 sucrose mutant enzyme D329L, pH 6.0, 35°C Klebsiella sp.
5.4.99.11 10400
-
 sucrose wild-type enzyme, pH 6.0, 35°C Klebsiella sp.
5.4.99.11 10430
-
 sucrose wild-type enzyme, pH 6.0, 35°C Klebsiella sp.