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Literature summary extracted from

  • Roessner, C.A.; Ponnamperuma, K.; Scott, A.I.
    Mutagenesis identifies a conserved tyrosine residue important for the activity of uroporphyrinogen III synthase from Anacystis nidulans (2002), FEBS Lett., 525, 25-28.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.75 overexpression as N-terminally his-tagged enzyme in Escherichia coli Synechococcus elongatus PCC 7942 = FACHB-805

Protein Variants

EC Number Protein Variants Comment Organism
4.2.1.75 D112G site-directed mutagenesis, 50% reduced activity compared to the wild-type enzyme Synechococcus elongatus PCC 7942 = FACHB-805
4.2.1.75 R15Q site-directed mutagenesis, unaltered activity level compared to the wild-type enzyme Synechococcus elongatus PCC 7942 = FACHB-805
4.2.1.75 S68A site-directed mutagenesis, unaltered activity level compared to the wild-type enzyme Synechococcus elongatus PCC 7942 = FACHB-805
4.2.1.75 T100A site-directed mutagenesis, 75% reduced activity compared to the wild-type enzyme Synechococcus elongatus PCC 7942 = FACHB-805
4.2.1.75 Y166F site-directed mutagenesis, more than 99% reduced activity compared to the wild-type enzyme Synechococcus elongatus PCC 7942 = FACHB-805

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.75 Synechococcus elongatus PCC 7942 = FACHB-805
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.75 recombinant His-tagged enzyme from Escherichia coli Synechococcus elongatus PCC 7942 = FACHB-805

Reaction

EC Number Reaction Comment Organism Reaction ID
4.2.1.75 hydroxymethylbilane = uroporphyrinogen III + H2O in the presence of hydroxymethylbilane synthase EC 4.3.1.8, enzyme forms uroporphyrinogen III from porphobilinogen, Tyr166 is essential for catalysis and/or substrate binding Synechococcus elongatus PCC 7942 = FACHB-805
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.75 0.25
-
 purified recombinant mutant Y166F Synechococcus elongatus PCC 7942 = FACHB-805
4.2.1.75 40
-
 purified recombinant wild-type Synechococcus elongatus PCC 7942 = FACHB-805

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.75 hydroxymethylbilane
-
 Synechococcus elongatus PCC 7942 = FACHB-805 uroporphyrinogen-III + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.2.1.75 U3S
-
 Synechococcus elongatus PCC 7942 = FACHB-805
4.2.1.75 Uroporphyrinogen III synthase
-
 Synechococcus elongatus PCC 7942 = FACHB-805

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.1.75 37
-
 assay at Synechococcus elongatus PCC 7942 = FACHB-805

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.75 8
-
 assay at Synechococcus elongatus PCC 7942 = FACHB-805