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Literature summary extracted from

  • Jacquin-Becker, C.; Ahel, I.; Ambrogelly, A.; Ruan, B.; Soll, D.; Stathopoulos, C.
    Cysteinyl-tRNA formation and prolyl-tRNA synthetase (2002), FEBS Lett., 514, 34-36.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
6.1.1.15 tRNA stimulates l-cysteine activation 2-3fold Methanocaldococcus jannaschii

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.1.1.15 gene proS, overexpression in Escherichia coli Methanocaldococcus jannaschii

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
6.1.1.15 crystal structure analysis Thermus thermophilus

Protein Variants

EC Number Protein Variants Comment Organism
6.1.1.15 E103A abolished activity with L-proline Methanocaldococcus jannaschii
6.1.1.15 additional information the dual-specific enzyme is sufficient for Cys-tRNACys production in a mutant disrupted in the gene encoing the canonical CysRS Methanococcus maripaludis
6.1.1.15 P100A highly reduced activity with L-cysteine Methanocaldococcus jannaschii

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.1.1.15 proline analogues
-
 Methanocaldococcus jannaschii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.1.1.15 ATP + L-cysteine + tRNAPro Methanocaldococcus jannaschii two-step reaction AMP + diphosphate + L-cysteinyl-tRNAPro
-
 r
6.1.1.15 ATP + L-proline + tRNAPro Thermus thermophilus
-
 AMP + diphosphate + L-prolyl-tRNAPro
-
 ?
6.1.1.15 ATP + L-proline + tRNAPro Giardia intestinalis
-
 AMP + diphosphate + L-prolyl-tRNAPro
-
 ?
6.1.1.15 ATP + L-proline + tRNAPro Methanocaldococcus jannaschii
-
 AMP + diphosphate + L-prolyl-tRNAPro
-
 r
6.1.1.15 ATP + L-proline + tRNAPro Methanococcus maripaludis
-
 AMP + diphosphate + L-prolyl-tRNAPro
-
 ?
6.1.1.15 additional information Methanocaldococcus jannaschii phylogenetic analysis ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.15 Giardia intestinalis
-
 enzyme with dual substrate specificity
-
6.1.1.15 Methanocaldococcus jannaschii
-
 enzyme with dual substrate specificity
-
6.1.1.15 Methanococcus maripaludis
-
 enzyme with dual substrate specificity
-
6.1.1.15 Thermus thermophilus
-
 enzyme with dual substrate specificity
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.1.1.15 recombinant from Escherichia coli Methanocaldococcus jannaschii

Reaction

EC Number Reaction Comment Organism Reaction ID
6.1.1.15 ATP + L-proline + tRNAPro = AMP + diphosphate + L-prolyl-tRNAPro enzyme with dual substrate specificity which utilizes L-proline and L-cysteine as substrates, discriminating mechanism Methanocaldococcus jannaschii
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.15 ATP + L-cysteine + tRNAPro two-step reaction Methanocaldococcus jannaschii AMP + diphosphate + L-cysteinyl-tRNAPro
-
 r
6.1.1.15 ATP + L-cysteine + tRNAPro dual-specificity enzyme Giardia intestinalis AMP + diphosphate + L-cysteinyl-tRNAPro
-
 ?
6.1.1.15 ATP + L-cysteine + tRNAPro dual-specificity enzyme Methanococcus maripaludis AMP + diphosphate + L-cysteinyl-tRNAPro
-
 ?
6.1.1.15 ATP + L-cysteine + tRNAPro dual-specificity enzyme, enzyme contains a discrete cysteine binding pocket Thermus thermophilus AMP + diphosphate + L-cysteinyl-tRNAPro
-
 ?
6.1.1.15 ATP + L-cysteine + tRNAPro two-step reaction, both steps are dependent on tRNACys Methanocaldococcus jannaschii AMP + diphosphate + L-cysteinyl-tRNAPro
-
 r
6.1.1.15 ATP + L-proline + tRNAPro
-
 Thermus thermophilus AMP + diphosphate + L-prolyl-tRNAPro
-
 ?
6.1.1.15 ATP + L-proline + tRNAPro
-
 Giardia intestinalis AMP + diphosphate + L-prolyl-tRNAPro
-
 ?
6.1.1.15 ATP + L-proline + tRNAPro
-
 Methanocaldococcus jannaschii AMP + diphosphate + L-prolyl-tRNAPro
-
 r
6.1.1.15 ATP + L-proline + tRNAPro
-
 Methanococcus maripaludis AMP + diphosphate + L-prolyl-tRNAPro
-
 ?
6.1.1.15 ATP + L-proline + tRNAPro two-step reaction Methanocaldococcus jannaschii AMP + diphosphate + L-prolyl-tRNAPro
-
 r
6.1.1.15 additional information the enzyme also performs the ATP-diphosphate exchange reaction, the enzyme possesses a pre- and post-editing mechanism for alanine Methanocaldococcus jannaschii ?
-
 ?
6.1.1.15 additional information phylogenetic analysis Methanocaldococcus jannaschii ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.1.1.15 Global RNA synthesis factor
-
 Thermus thermophilus
6.1.1.15 Global RNA synthesis factor
-
 Giardia intestinalis
6.1.1.15 Global RNA synthesis factor
-
 Methanocaldococcus jannaschii
6.1.1.15 Global RNA synthesis factor
-
 Methanococcus maripaludis
6.1.1.15 Pro-tRNA synthetase
-
 Thermus thermophilus
6.1.1.15 Pro-tRNA synthetase
-
 Giardia intestinalis
6.1.1.15 Pro-tRNA synthetase
-
 Methanocaldococcus jannaschii
6.1.1.15 Pro-tRNA synthetase
-
 Methanococcus maripaludis
6.1.1.15 Proline translase
-
 Thermus thermophilus
6.1.1.15 Proline translase
-
 Giardia intestinalis
6.1.1.15 Proline translase
-
 Methanocaldococcus jannaschii
6.1.1.15 Proline translase
-
 Methanococcus maripaludis
6.1.1.15 Proline--tRNA ligase
-
 Thermus thermophilus
6.1.1.15 Proline--tRNA ligase
-
 Giardia intestinalis
6.1.1.15 Proline--tRNA ligase
-
 Methanocaldococcus jannaschii
6.1.1.15 Proline--tRNA ligase
-
 Methanococcus maripaludis
6.1.1.15 Prolyl RNA synthetase
-
 Thermus thermophilus
6.1.1.15 Prolyl RNA synthetase
-
 Giardia intestinalis
6.1.1.15 Prolyl RNA synthetase
-
 Methanocaldococcus jannaschii
6.1.1.15 Prolyl RNA synthetase
-
 Methanococcus maripaludis
6.1.1.15 Prolyl-transfer ribonucleate synthetase
-
 Thermus thermophilus
6.1.1.15 Prolyl-transfer ribonucleate synthetase
-
 Giardia intestinalis
6.1.1.15 Prolyl-transfer ribonucleate synthetase
-
 Methanocaldococcus jannaschii
6.1.1.15 Prolyl-transfer ribonucleate synthetase
-
 Methanococcus maripaludis
6.1.1.15 Prolyl-transfer ribonucleic acid synthetase
-
 Thermus thermophilus
6.1.1.15 Prolyl-transfer ribonucleic acid synthetase
-
 Giardia intestinalis
6.1.1.15 Prolyl-transfer ribonucleic acid synthetase
-
 Methanocaldococcus jannaschii
6.1.1.15 Prolyl-transfer ribonucleic acid synthetase
-
 Methanococcus maripaludis
6.1.1.15 Prolyl-transfer RNA synthetase
-
 Thermus thermophilus
6.1.1.15 Prolyl-transfer RNA synthetase
-
 Giardia intestinalis
6.1.1.15 Prolyl-transfer RNA synthetase
-
 Methanocaldococcus jannaschii
6.1.1.15 Prolyl-transfer RNA synthetase
-
 Methanococcus maripaludis
6.1.1.15 Prolyl-tRNA synthetase
-
 Thermus thermophilus
6.1.1.15 Prolyl-tRNA synthetase
-
 Giardia intestinalis
6.1.1.15 Prolyl-tRNA synthetase
-
 Methanocaldococcus jannaschii
6.1.1.15 Prolyl-tRNA synthetase
-
 Methanococcus maripaludis
6.1.1.15 ProRS
-
 Thermus thermophilus
6.1.1.15 ProRS
-
 Giardia intestinalis
6.1.1.15 ProRS
-
 Methanocaldococcus jannaschii
6.1.1.15 ProRS
-
 Methanococcus maripaludis

Cofactor

EC Number Cofactor Comment Organism Structure
6.1.1.15 ATP
-
 Thermus thermophilus
6.1.1.15 ATP
-
 Giardia intestinalis
6.1.1.15 ATP
-
 Methanocaldococcus jannaschii
6.1.1.15 ATP
-
 Methanococcus maripaludis