EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.1.1.74 | 0.009 | - |
Indole-3-acetaldehyde | 70°C, pH 8.0 | Sulfolobus sp. | |
4.1.1.74 | 0.042 | - |
glyceraldehyde | 70°C, pH 8.0 | Sulfolobus sp. | |
4.1.1.74 | 0.105 | - |
phenylacetaldehyde | 70°C, pH 8.0 | Sulfolobus sp. | |
4.1.1.74 | 0.395 | - |
benzaldehyde | 70°C, pH 8.0 | Sulfolobus sp. | |
4.1.1.74 | 0.788 | - |
Indole-3-pyruvate | 70°C, pH 8.0 | Sulfolobus sp. | |
4.1.1.74 | 1.66 | - |
phenylpyruvate | 70°C, pH 8.0 | Sulfolobus sp. |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
4.1.1.74 | Fe2+ | 8.7 mol per mol of enzyme | Sulfolobus sp. | |
4.1.1.74 | Mo2+ | 0.8 mol per mol of enzyme | Sulfolobus sp. |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
4.1.1.74 | 19000 | - |
2 * 89000, alpha, + 2 * 30000, beta, + 2 * 19000, gamma, SDS-PAGE | Sulfolobus sp. |
4.1.1.74 | 30000 | - |
2 * 89000, alpha, + 2 * 30000, beta, + 2 * 19000, gamma, SDS-PAGE | Sulfolobus sp. |
4.1.1.74 | 89000 | - |
2 * 89000, alpha, + 2 * 30000, beta, + 2 * 19000, gamma, SDS-PAGE | Sulfolobus sp. |
4.1.1.74 | 280000 | - |
gel filtration | Sulfolobus sp. |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.1.1.74 | Sulfolobus sp. | - |
- |
- |
4.1.1.74 | Sulfolobus sp. 7 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.1.1.74 | - |
Sulfolobus sp. |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
4.1.1.74 | 3-(indol-3-yl)pyruvate = 2-(indol-3-yl)acetaldehyde + CO2 | mechanism | Sulfolobus sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.1.1.74 | 2-oxoglutarate | - |
Sulfolobus sp. | ? | - |
? | |
4.1.1.74 | 2-oxoglutarate | - |
Sulfolobus sp. 7 | ? | - |
? | |
4.1.1.74 | 2-oxoisovalerate | - |
Sulfolobus sp. | ? | - |
? | |
4.1.1.74 | 2-oxoisovalerate | - |
Sulfolobus sp. 7 | ? | - |
? | |
4.1.1.74 | 3-(indol-3-yl)pyruvate | - |
Sulfolobus sp. | 2-(indol-3-yl)acetaldehyde + CO2 | - |
? | |
4.1.1.74 | 3-(indol-3-yl)pyruvate | - |
Sulfolobus sp. 7 | 2-(indol-3-yl)acetaldehyde + CO2 | - |
? | |
4.1.1.74 | benzaldehyde | - |
Sulfolobus sp. | ? | - |
? | |
4.1.1.74 | glyceraldehyde | - |
Sulfolobus sp. | ? | - |
? | |
4.1.1.74 | indole-3-acetaldehyde | - |
Sulfolobus sp. | ? | - |
? | |
4.1.1.74 | indole-3-acetaldehyde | - |
Sulfolobus sp. 7 | ? | - |
? | |
4.1.1.74 | phenylacetaldehyde | - |
Sulfolobus sp. | ? | - |
? | |
4.1.1.74 | phenylpyruvate | - |
Sulfolobus sp. | phenylethanal + CO2 | - |
? | |
4.1.1.74 | pyruvate | - |
Sulfolobus sp. | acetaldehyde + CO2 | - |
? | |
4.1.1.74 | pyruvate | - |
Sulfolobus sp. 7 | acetaldehyde + CO2 | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
4.1.1.74 | hexamer | 2 * 89000, alpha, + 2 * 30000, beta, + 2 * 19000, gamma, SDS-PAGE | Sulfolobus sp. |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.1.1.74 | 70 | - |
30 min, pH 7.0, almost stable | Sulfolobus sp. |
4.1.1.74 | 90 | - |
30 min, 22% inactivation | Sulfolobus sp. |
4.1.1.74 | 100 | - |
30 min, 97% inactivation | Sulfolobus sp. |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
4.1.1.74 | 8 | - |
70% loss of activity at pH 7.0, 15% loss of activity at pH 9 | Sulfolobus sp. |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
4.1.1.74 | biotin | binds possibly to beta subunit | Sulfolobus sp. | |
4.1.1.74 | FMN | 1.9 mol per mol of enzyme | Sulfolobus sp. | |
4.1.1.74 | additional information | no thiamine diphosphate | Sulfolobus sp. |