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Literature summary extracted from

  • Wakagi, T.; Fukuda, E.; Ogawa, Y.; Kino, H.; Matsuzawa, H.
    A novel bifunctional molybdo-enzyme catalyzing both decarboxylation of indolepyruvate and oxidation of indoleacetaldehyde from a thermoacidophilic archaeon, Sulfolobus sp. strain 7 (2002), FEBS Lett., 510, 196-200.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.74 0.009
-
Indole-3-acetaldehyde 70°C, pH 8.0 Sulfolobus sp.
4.1.1.74 0.042
-
glyceraldehyde 70°C, pH 8.0 Sulfolobus sp.
4.1.1.74 0.105
-
phenylacetaldehyde 70°C, pH 8.0 Sulfolobus sp.
4.1.1.74 0.395
-
benzaldehyde 70°C, pH 8.0 Sulfolobus sp.
4.1.1.74 0.788
-
Indole-3-pyruvate 70°C, pH 8.0 Sulfolobus sp.
4.1.1.74 1.66
-
phenylpyruvate 70°C, pH 8.0 Sulfolobus sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.1.74 Fe2+ 8.7 mol per mol of enzyme Sulfolobus sp.
4.1.1.74 Mo2+ 0.8 mol per mol of enzyme Sulfolobus sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.74 19000
-
2 * 89000, alpha, + 2 * 30000, beta, + 2 * 19000, gamma, SDS-PAGE Sulfolobus sp.
4.1.1.74 30000
-
2 * 89000, alpha, + 2 * 30000, beta, + 2 * 19000, gamma, SDS-PAGE Sulfolobus sp.
4.1.1.74 89000
-
2 * 89000, alpha, + 2 * 30000, beta, + 2 * 19000, gamma, SDS-PAGE Sulfolobus sp.
4.1.1.74 280000
-
gel filtration Sulfolobus sp.

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.74 Sulfolobus sp.
-
-
-
4.1.1.74 Sulfolobus sp. 7
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.74
-
Sulfolobus sp.

Reaction

EC Number Reaction Comment Organism Reaction ID
4.1.1.74 3-(indol-3-yl)pyruvate = 2-(indol-3-yl)acetaldehyde + CO2 mechanism Sulfolobus sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.74 2-oxoglutarate
-
Sulfolobus sp. ?
-
?
4.1.1.74 2-oxoglutarate
-
Sulfolobus sp. 7 ?
-
?
4.1.1.74 2-oxoisovalerate
-
Sulfolobus sp. ?
-
?
4.1.1.74 2-oxoisovalerate
-
Sulfolobus sp. 7 ?
-
?
4.1.1.74 3-(indol-3-yl)pyruvate
-
Sulfolobus sp. 2-(indol-3-yl)acetaldehyde + CO2
-
?
4.1.1.74 3-(indol-3-yl)pyruvate
-
Sulfolobus sp. 7 2-(indol-3-yl)acetaldehyde + CO2
-
?
4.1.1.74 benzaldehyde
-
Sulfolobus sp. ?
-
?
4.1.1.74 glyceraldehyde
-
Sulfolobus sp. ?
-
?
4.1.1.74 indole-3-acetaldehyde
-
Sulfolobus sp. ?
-
?
4.1.1.74 indole-3-acetaldehyde
-
Sulfolobus sp. 7 ?
-
?
4.1.1.74 phenylacetaldehyde
-
Sulfolobus sp. ?
-
?
4.1.1.74 phenylpyruvate
-
Sulfolobus sp. phenylethanal + CO2
-
?
4.1.1.74 pyruvate
-
Sulfolobus sp. acetaldehyde + CO2
-
?
4.1.1.74 pyruvate
-
Sulfolobus sp. 7 acetaldehyde + CO2
-
?

Subunits

EC Number Subunits Comment Organism
4.1.1.74 hexamer 2 * 89000, alpha, + 2 * 30000, beta, + 2 * 19000, gamma, SDS-PAGE Sulfolobus sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.1.1.74 70
-
30 min, pH 7.0, almost stable Sulfolobus sp.
4.1.1.74 90
-
30 min, 22% inactivation Sulfolobus sp.
4.1.1.74 100
-
30 min, 97% inactivation Sulfolobus sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.1.74 8
-
70% loss of activity at pH 7.0, 15% loss of activity at pH 9 Sulfolobus sp.

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.1.74 biotin binds possibly to beta subunit Sulfolobus sp.
4.1.1.74 FMN 1.9 mol per mol of enzyme Sulfolobus sp.
4.1.1.74 additional information no thiamine diphosphate Sulfolobus sp.