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Literature summary extracted from

  • Pernas, M.A.; Lopez, C.; Rua, M.L.; Hermoso, J.
    Influence of the conformational flexibility on the kinetics and dimerization process of two Candida rugosa lipase isoenzymes (2001), FEBS Lett., 501, 87-91.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.3 sodium deoxycholate isozymes Lip3 and Lip1, inhibition kinetics Diutina rugosa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.3 additional information
-
 additional information kinetics Diutina rugosa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.3 43000
-
 monomeric Lip3, gel filtration Diutina rugosa
3.1.1.3 60000
-
 1 * 60000, Lip3, SDS-PAGE Diutina rugosa
3.1.1.3 60000
-
 2 * 60000, Lip3, SDS-PAGE Diutina rugosa
3.1.1.3 117000
-
 dimeric Lip3, gel filtration Diutina rugosa

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.3 Diutina rugosa
-
 2 isoenzymes Lip1 and Lip3
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.3 910
-
 purified Lip3 monomer Diutina rugosa
3.1.1.3 1030
-
 purified Lip3 dimer Diutina rugosa
3.1.1.3 1500
-
 purified Lip1 dimer Diutina rugosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.3 triacetin + H2O
-
 Diutina rugosa diacetin + acetic acid
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.3 dimer 2 * 60000, Lip3, SDS-PAGE Diutina rugosa
3.1.1.3 monomer 1 * 60000, Lip3, SDS-PAGE Diutina rugosa
3.1.1.3 More study of dimerisation process of lipase isozymes, isozyme Lip3 exists in a dimeric and a monomeric form Diutina rugosa

Synonyms

EC Number Synonyms Comment Organism
3.1.1.3 lipase
-
 Diutina rugosa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.3 30
-
 assay at Diutina rugosa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.3 7
-
 assay at Diutina rugosa

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.1.1.3 Diutina rugosa Lip3 monomer, isoelectric focusing
-
 5.8