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Literature summary extracted from

  • Yoko-o, T.; Tsukahara, K.; Watanabe, T.; Hata-Sugi, N.; Yoshimatsu, K.; Nagasu, T.; Jigami, Y.
    Schizosaccharomyces pombe och1+ encodes alpha-1,6-mannosyltransferase that is involved in outer chain elongation of N-linked oligosaccharides (2001), FEBS Lett., 489, 75-80.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.232 och1+ gene, sequencing Schizosaccharomyces pombe

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.232 additional information disruption mutant of och1+: viable, slow-growing phenotype, temperature- and hygromycin B-sensitive, no synthesis of outer chains on acid phosphatase Schizosaccharomyces pombe
2.4.1.232 W266stop inactive mutant strain 7X4 with a nonsense mutation resulting in a truncated Och1p, temperature- and hygromycin B-sensitive, no synthesis of outer chains on acid phosphatase Schizosaccharomyces pombe

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.232 additional information Schizosaccharomyces pombe SpOch1p is a key enzyme in outer chain elongation of N-linked oligosaccharides on glycoproteins ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.232 Schizosaccharomyces pombe Q9UTR6 strains JY741 and L972
-

Reaction

EC Number Reaction Comment Organism Reaction ID
2.4.1.232 GDP-mannose + Man8GlcNAc = GDP + Man9GlcNAc requires Mn2+, in Saccharomyces cerevisiae, this enzyme catalyses an essential step in the outer chain elongation of N-linked oligosaccharides, Man8GlcNAc and Man9GlcNAc are equally good substrates Schizosaccharomyces pombe
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.232 additional information
-
-
 Schizosaccharomyces pombe

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.232 GDP-mannose + pyridylaminated Man5GlcNAc2 incorporates alpha-1,6-linked mannose, poor substrate Schizosaccharomyces pombe GDP + pyridylaminated Man6GlcNAc2
-
 ?
2.4.1.232 GDP-mannose + pyridylaminated Man6GlcNAc2 incorporates alpha-1,6-linked mannose Schizosaccharomyces pombe GDP + pyridylaminated Man7GlcNAc2
-
 ?
2.4.1.232 GDP-mannose + pyridylaminated Man7GlcNAc2 incorporates alpha-1,6-linked mannose Schizosaccharomyces pombe GDP + pyridylaminated Man8GlcNAc2
-
 ?
2.4.1.232 GDP-mannose + pyridylaminated Man8GlcNAc2 incorporates alpha-1,6-linked mannose Schizosaccharomyces pombe GDP + pyridylaminated Man9GlcNAc2
-
 ?
2.4.1.232 GDP-mannose + pyridylaminated Man9GlcNAc2 incorporates alpha-1,6-linked mannose Schizosaccharomyces pombe GDP + pyridylaminated Man10GlcNAc2
-
 ?
2.4.1.232 GDP-mannose + pyridylaminated oligosaccharide acceptor incorporates alpha-1,6-linked mannose, substrate specificity Schizosaccharomyces pombe GDP + pyridylaminated alpha-mannosyl-1,6-oligosaccharide
-
 ?
2.4.1.232 additional information SpOch1p has a wider substrate specificity than ScOch1p from Saccharomyces cerevisiae Schizosaccharomyces pombe ?
-
 ?
2.4.1.232 additional information SpOch1p is a key enzyme in outer chain elongation of N-linked oligosaccharides on glycoproteins Schizosaccharomyces pombe ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.232 alpha-1,6-mannosyltransferase
-
 Schizosaccharomyces pombe
2.4.1.232 GDP-mannose:glycolipid 1,6-alpha-D-mannosyltransferase
-
 Schizosaccharomyces pombe
2.4.1.232 glycolipid 6-alpha-mannosyltransferase
-
 Schizosaccharomyces pombe
2.4.1.232 glycoprotein alpha1-6-mannosyltransferase
-
 Schizosaccharomyces pombe
2.4.1.232 mannosyltransferase, guanosine diphosphomannose-glycoprotein alpha1-6-
-
 Schizosaccharomyces pombe
2.4.1.232 och1+
-
 Schizosaccharomyces pombe
2.4.1.232 SpOch1p och1+ gene product, Schizosaccharomyces pombe Schizosaccharomyces pombe

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.232 30
-
 assay at Schizosaccharomyces pombe

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.232 7.5
-
 assay at Schizosaccharomyces pombe