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Literature summary extracted from

  • Bisso, A.; Sturla, L.; Zanardi, D.; De Flora, A.; Tonetti, M.
    Structural and enzymatic characterization of human recombinant GDP-D-mannose-4,6-dehydratase (1999), FEBS Lett., 456, 370-374.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.47 L-GMD, S-GMD and M-GMD, expressed in Escherichia coli Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.47 GDP competitive Homo sapiens
4.2.1.47 GDP-L-fucose non-competitive Homo sapiens
4.2.1.47 GDP-L-glucose competitive Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.47 0.0035
-
 GDP-mannose pH 8, isoenzyme M-GMD Homo sapiens
4.2.1.47 0.00364
-
 GDP-mannose pH 8, isoenzyme L-GMD Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.47 38700
-
 isoenzyme S-GMD, SDS-PAGE Homo sapiens
4.2.1.47 40200
-
 isoenzyme M-GMD, SDS-PAGE Homo sapiens
4.2.1.47 42000
-
 isoenzyme L-GMD, SDS-PAGE Homo sapiens
4.2.1.47 80000
-
 isoenzyme M-GMD, dimeric form, gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.47 additional information Homo sapiens key step in the de novo pathway of GDP-L-fucose biosynthesis ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.47 Homo sapiens O60547
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.47 only isoenzyme L-GMD and isoenzyme M-GMD are soluble, isoenzyme S-GMD is inactive and forms a precipitate Homo sapiens

Storage Stability

EC Number Storage Stability Organism
4.2.1.47 4°C, presence of reducing agents, 36 h, 50% loss of activity Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.47 GDP-mannose
-
 Homo sapiens GDP-4-dehydro-6-deoxy-D-mannose + H2O
-
 ?
4.2.1.47 additional information key step in the de novo pathway of GDP-L-fucose biosynthesis Homo sapiens ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.1.47 dimer L-GMD and M-GMD form a homodimer, M-GMD: 2 * 40200, SDS-PAGE Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
4.2.1.47 L-GMD isoenzyme Homo sapiens
4.2.1.47 M-GMD isoenzyme Homo sapiens
4.2.1.47 S-GMD isoenzyme Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2.1.47 0.8
-
 GDP-mannose pH 8, M-GMD and L-GMD Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.47 7.5
-
-
 Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
4.2.1.47 NADP+ tightly bound, 1 mol per homodimer Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.2.1.47 0.0113
-
 GDP-L-fucose pH 8 Homo sapiens