EC Number | Cloned (Comment) | Organism |
---|---|---|
3.4.24.27 | cloned in Bacillus subtilis DB117, plasmids subcloned in Escherichia coli | Bacillus thermoproteolyticus |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.4.24.27 | D226A | site-directed mutagenesis | Bacillus thermoproteolyticus |
3.4.24.27 | Y157F | site-directed mutagenesis | Bacillus thermoproteolyticus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.4.24.27 | HACBO-Gly | - |
Bacillus thermoproteolyticus | |
3.4.24.27 | phosphoramidon | - |
Bacillus thermoproteolyticus | |
3.4.24.27 | thiorphan | - |
Bacillus thermoproteolyticus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.24.27 | 0.21 | - |
leucine enkephalin | pH 7.4, 37°C, wild-type | Bacillus thermoproteolyticus | |
3.4.24.27 | 0.48 | - |
leucine enkephalin | pH 7.4, 37°C, mutant Y157F | Bacillus thermoproteolyticus | |
3.4.24.27 | 1.985 | - |
leucine enkephalin | pH 7.4, 37°C, mutant D226A | Bacillus thermoproteolyticus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.4.24.27 | 36000 | - |
Western blot | Bacillus thermoproteolyticus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.4.24.27 | Bacillus thermoproteolyticus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.4.24.27 | recombinant wild-type and mutated thermolysin | Bacillus thermoproteolyticus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.24.27 | leucine enkephalin + H2O | - |
Bacillus thermoproteolyticus | ? | - |
? |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.24.27 | 2.94 | - |
leucine enkephalin | pH 7.4, 37°C, mutant Y157F | Bacillus thermoproteolyticus | |
3.4.24.27 | 155.5 | - |
leucine enkephalin | pH 7.4, 37°C, wild-type | Bacillus thermoproteolyticus | |
3.4.24.27 | 156 | - |
leucine enkephalin | pH 7.4, 37°C, wild-type | Bacillus thermoproteolyticus |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.24.27 | 0.000031 | - |
phosphoramidon | pH 7.4, 37°C, mutant Y157F | Bacillus thermoproteolyticus | |
3.4.24.27 | 0.00006 | - |
phosphoramidon | pH 7.4, 37°C, wild-type | Bacillus thermoproteolyticus | |
3.4.24.27 | 0.00019 | - |
phosphoramidon | pH 7.4, 37°C, mutant D226A | Bacillus thermoproteolyticus | |
3.4.24.27 | 0.0016 | - |
thiorphan | pH 7.4, 37°C, wild-type | Bacillus thermoproteolyticus | |
3.4.24.27 | 0.0031 | - |
HACBO-Gly | pH 7.4, 37°C, wild-type | Bacillus thermoproteolyticus | |
3.4.24.27 | 0.0076 | - |
thiorphan | pH 7.4, 37°C, mutant Y157F | Bacillus thermoproteolyticus | |
3.4.24.27 | 0.013 | - |
thiorphan | pH 7.4, 37°C, mutant D226A | Bacillus thermoproteolyticus | |
3.4.24.27 | 0.019 | - |
HACBO-Gly | pH 7.4, 37°C, mutant D226A | Bacillus thermoproteolyticus | |
3.4.24.27 | 0.031 | - |
HACBO-Gly | pH 7.4, 37°C, mutant Y157F | Bacillus thermoproteolyticus |