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Literature summary extracted from

  • Marie-Claire, C.; Ruffet, E.; Tiraboschi, G.; Fournie-Zaluski, M.C.
    Differences in transition state stabilization between thermolysin (EC 3.4.24.27) and neprilysin (EC 3.4.24.11) (1998), FEBS Lett., 438, 215-219.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.24.27 cloned in Bacillus subtilis DB117, plasmids subcloned in Escherichia coli Bacillus thermoproteolyticus

Protein Variants

EC Number Protein Variants Comment Organism
3.4.24.27 D226A site-directed mutagenesis Bacillus thermoproteolyticus
3.4.24.27 Y157F site-directed mutagenesis Bacillus thermoproteolyticus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.27 HACBO-Gly
-
Bacillus thermoproteolyticus
3.4.24.27 phosphoramidon
-
Bacillus thermoproteolyticus
3.4.24.27 thiorphan
-
Bacillus thermoproteolyticus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.24.27 0.21
-
leucine enkephalin pH 7.4, 37°C, wild-type Bacillus thermoproteolyticus
3.4.24.27 0.48
-
leucine enkephalin pH 7.4, 37°C, mutant Y157F Bacillus thermoproteolyticus
3.4.24.27 1.985
-
leucine enkephalin pH 7.4, 37°C, mutant D226A Bacillus thermoproteolyticus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.24.27 36000
-
Western blot Bacillus thermoproteolyticus

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.27 Bacillus thermoproteolyticus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.24.27 recombinant wild-type and mutated thermolysin Bacillus thermoproteolyticus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.27 leucine enkephalin + H2O
-
Bacillus thermoproteolyticus ?
-
?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.24.27 2.94
-
leucine enkephalin pH 7.4, 37°C, mutant Y157F Bacillus thermoproteolyticus
3.4.24.27 155.5
-
leucine enkephalin pH 7.4, 37°C, wild-type Bacillus thermoproteolyticus
3.4.24.27 156
-
leucine enkephalin pH 7.4, 37°C, wild-type Bacillus thermoproteolyticus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.4.24.27 0.000031
-
phosphoramidon pH 7.4, 37°C, mutant Y157F Bacillus thermoproteolyticus
3.4.24.27 0.00006
-
phosphoramidon pH 7.4, 37°C, wild-type Bacillus thermoproteolyticus
3.4.24.27 0.00019
-
phosphoramidon pH 7.4, 37°C, mutant D226A Bacillus thermoproteolyticus
3.4.24.27 0.0016
-
thiorphan pH 7.4, 37°C, wild-type Bacillus thermoproteolyticus
3.4.24.27 0.0031
-
HACBO-Gly pH 7.4, 37°C, wild-type Bacillus thermoproteolyticus
3.4.24.27 0.0076
-
thiorphan pH 7.4, 37°C, mutant Y157F Bacillus thermoproteolyticus
3.4.24.27 0.013
-
thiorphan pH 7.4, 37°C, mutant D226A Bacillus thermoproteolyticus
3.4.24.27 0.019
-
HACBO-Gly pH 7.4, 37°C, mutant D226A Bacillus thermoproteolyticus
3.4.24.27 0.031
-
HACBO-Gly pH 7.4, 37°C, mutant Y157F Bacillus thermoproteolyticus