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Literature summary extracted from

  • Halfon, S.; Patel, S.; Vega, F.; Zurawski, S.; Zurawski, G.
    Autocatalytic activation of human legumain at aspartic acid residues (1998), FEBS Lett., 438, 114-118.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.22.34 DNA and amino acid sequence determination and analysis Mus musculus
3.4.22.34 overexpression in murine cell line COP5 as a C-terminally IgG-fusion protein Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
3.4.22.34 C52A reduced autocatalytic activity, no activity against other substrates Homo sapiens
3.4.22.34 H150A reduced autocatalytic activity, no activity against other substrates Homo sapiens
3.4.22.34 H47A reduced autocatalytic activity, no activity against other substrates Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.22.34 additional information
-
 additional information
-
 Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.22.34 48000
-
 x * 48000, autocatalytically processed enzyme, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.22.34 protein + H2O Homo sapiens
-
 peptides
-
 ?
3.4.22.34 protein + H2O Mus musculus
-
 peptides
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.34 Homo sapiens
-
-
-
3.4.22.34 Mus musculus O89017
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.22.34 proteolytic modification autocatalytic activation by cleavage of aspartic acid residues, overview Homo sapiens

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.22.34 recombinant IgG-fusion protein from cell line COP5 Homo sapiens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.22.34 additional information
-
-
 Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.34 benzyloxycarbonyl-Ala-Ala-Asn-4-methylcoumarin-7-amide + H2O
-
 Homo sapiens benzyloxycarbonyl-Ala-Ala-Asn + 7-amino-4-methylcoumarin
-
 ?
3.4.22.34 legumain + H2O autocatalysis Homo sapiens processed legumain + N-terminal and C-terminal prodomains
-
 ?
3.4.22.34 additional information no activity with benzyloxycarbonyl-Gly-Ala-Gln-4-methylcoumarin-7-amide Homo sapiens ?
-
 ?
3.4.22.34 protein + H2O
-
 Homo sapiens peptides
-
 ?
3.4.22.34 protein + H2O
-
 Mus musculus peptides
-
 ?
3.4.22.34 succinyl-Tyr-Val-Ala-Asn-4-methylcoumarin-7-amide + H2O
-
 Homo sapiens succinyl-Tyr-Val-Ala-Asn + 7-amino-4-methylcoumarin
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.22.34 ? x * 48000, autocatalytically processed enzyme, SDS-PAGE Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.22.34 25
-
 assay at Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.22.34 additional information
-
 additional information
-
 Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.22.34 6.1
-
 recombinant enzyme, dependent on buffer system Homo sapiens