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Literature summary extracted from

  • Kim, S.; Jo, Y.J.; Lee, S.H.; Motegi, H.; Shiba, K.; Sassanfar, M.; Martinis, S.A.
    Biochemical and phylogenetic analyses of methionyl-tRNA synthetase isolated from a pathogenic microorganism, Mycobacterium tuberculosis (1998), FEBS Lett., 427, 259-262.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.1.1.10 DNA sequence determination and analysis, overexpression in Escherichia coli Mycobacterium tuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.1.1.10 0.0032
-
 tRNAMet substrate from Escherichia coli, 37°C Mycobacterium tuberculosis
6.1.1.10 1
-
 tRNAMet recombinant C-terminally truncated enzyme, 37°C Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.1.1.10 additional information enzyme lacks Zn2+ binding motif and contains no Zn2+ Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.1.1.10 ATP + L-methionine + tRNAMet Escherichia coli
-
 AMP + diphosphate + L-methionyl-tRNAMet
-
 ?
6.1.1.10 ATP + L-methionine + tRNAMet Mycobacterium tuberculosis
-
 AMP + diphosphate + L-methionyl-tRNAMet
-
 ?
6.1.1.10 additional information Escherichia coli phylogenetic analysis ?
-
 ?
6.1.1.10 additional information Mycobacterium tuberculosis phylogenetic analysis ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.10 Escherichia coli
-
 purified recombinant C-terminally truncated enzyme
-
6.1.1.10 Mycobacterium tuberculosis
-
 non-typical class I enzyme lacking the Zn2+ binding motif and the C-terminal dimerization appendix which is found in enzymes from other organisms
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.10 ATP + L-methionine + tRNAMet
-
 Escherichia coli AMP + diphosphate + L-methionyl-tRNAMet
-
 ?
6.1.1.10 ATP + L-methionine + tRNAMet
-
 Mycobacterium tuberculosis AMP + diphosphate + L-methionyl-tRNAMet
-
 ?
6.1.1.10 ATP + L-methionine + tRNAMet also utilizes tRNAMet from Escherichia coli Mycobacterium tuberculosis AMP + diphosphate + L-methionyl-tRNAMet
-
 ?
6.1.1.10 additional information phylogenetic analysis Escherichia coli ?
-
 ?
6.1.1.10 additional information phylogenetic analysis Mycobacterium tuberculosis ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.1.1.10 Methionine translase
-
 Escherichia coli
6.1.1.10 Methionine translase
-
 Mycobacterium tuberculosis
6.1.1.10 Methionine--tRNA ligase
-
 Escherichia coli
6.1.1.10 Methionine--tRNA ligase
-
 Mycobacterium tuberculosis
6.1.1.10 Methionyl tRNA synthetase
-
 Escherichia coli
6.1.1.10 Methionyl tRNA synthetase
-
 Mycobacterium tuberculosis
6.1.1.10 Methionyl-transfer ribonucleate synthetase
-
 Escherichia coli
6.1.1.10 Methionyl-transfer ribonucleate synthetase
-
 Mycobacterium tuberculosis
6.1.1.10 Methionyl-transfer ribonucleic acid synthetase
-
 Escherichia coli
6.1.1.10 Methionyl-transfer ribonucleic acid synthetase
-
 Mycobacterium tuberculosis
6.1.1.10 Methionyl-transfer RNA synthetase
-
 Escherichia coli
6.1.1.10 Methionyl-transfer RNA synthetase
-
 Mycobacterium tuberculosis
6.1.1.10 MetRS
-
 Escherichia coli
6.1.1.10 MetRS
-
 Mycobacterium tuberculosis
6.1.1.10 Synthetase, methionyl-transfer ribonucleate
-
 Escherichia coli
6.1.1.10 Synthetase, methionyl-transfer ribonucleate
-
 Mycobacterium tuberculosis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.1.1.10 37
-
 assay at Escherichia coli
6.1.1.10 37
-
 assay at Mycobacterium tuberculosis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.1.1.10 2.83
-
 tRNAMet substrate from Escherichia coli, 37°C Mycobacterium tuberculosis
6.1.1.10 3 6 tRNAMet substrate from Escherichia coli, 37°C Mycobacterium tuberculosis
6.1.1.10 6.85
-
 tRNAMet recombinant C-terminally truncated enzyme, 37°C Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
6.1.1.10 ATP
-
 Escherichia coli
6.1.1.10 ATP
-
 Mycobacterium tuberculosis