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Literature summary extracted from
- Holliday junction-resolving enzymes from eight hyperthermophilic archaea differ in reactions with cruciform DNA (2002), Extremophiles, 6, 359-367.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.1.21.10 | overexpression in Escherichia coli BL21 | Methanothermobacter thermautotrophicus |
| 3.1.21.10 | overexpression in Escherichia coli BL21 | Methanocaldococcus jannaschii |
| 3.1.21.10 | overexpression in Escherichia coli BL21 | Archaeoglobus fulgidus |
| 3.1.21.10 | overexpression in Escherichia coli BL21 | Pyrococcus horikoshii |
| 3.1.21.10 | overexpression in Escherichia coli BL21 | Pyrococcus abyssi |
| 3.1.21.10 | overexpression in Escherichia coli BL21 | Aeropyrum pernix |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.21.10 | Aeropyrum pernix | Q9YEX2 | - |
- |
| 3.1.21.10 | Archaeoglobus fulgidus | - |
- |
- |
| 3.1.21.10 | Methanocaldococcus jannaschii | - |
- |
- |
| 3.1.21.10 | Methanothermobacter thermautotrophicus | - |
- |
- |
| 3.1.21.10 | Pyrococcus abyssi | Q9V0H1 | - |
- |
| 3.1.21.10 | Pyrococcus horikoshii | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.21.10 | - |
Methanothermobacter thermautotrophicus |
| 3.1.21.10 | - |
Methanocaldococcus jannaschii |
| 3.1.21.10 | - |
Archaeoglobus fulgidus |
| 3.1.21.10 | - |
Pyrococcus horikoshii |
| 3.1.21.10 | - |
Pyrococcus abyssi |
| 3.1.21.10 | - |
Aeropyrum pernix |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.21.10 | DNA + H2O | cleaves the Holliday junction in a symmetrical mode by introducing two nicks in opposite strands across the junction. Cleavage of fixed cruciform DNA CFK1a11 and CFK1a01 which allows a branch migration over ten nucleotides. The pair of opposite strands are 01/0.3 and 02/04 in CFK1a01 and 11/13 and 12/14 in CFK1a11. CFK1a11 is cleaved at identical positions, three nucleotides 3' of the junction. Strong bias for cleavage axis 12/14 with more than 80% of the substrate being cleaved, while only less than 10% along axis 11713 is cleaved. CFK1a01 is cleaved along both axes with equal efficiency, cleavage positions | Methanothermobacter thermautotrophicus | ? | - |
? |  |
| 3.1.21.10 | DNA + H2O | cleaves the Holliday junction in a symmetrical mode by introducing two nicks in opposite strands across the junction. Cleavage of fixed cruciform DNA CFK1a11 and CFK1a01 which allows a branch migration over ten nucleotides. The pair of opposite strands are 01/0.3 and 02/04 in CFK1a01 and 11/13 and 12/14 in CFK1a11. CFK1a11 is cleaved at identical positions, three nucleotides 3' of the junction. Strong bias for cleavage axis 12/14 with more than 80% of the substrate being cleaved, while only less than 10% along axis 11713 is cleaved. CFK1a01 is cleaved along both axes with equal efficiency, cleavage positions | Pyrococcus horikoshii | ? | - |
? | |
| 3.1.21.10 | DNA + H2O | cleaves the Holliday junction in a symmetrical mode by introducing two nicks in opposite strands across the junction. Cleavage of fixed cruciform DNA CFK1a11 and CFK1a01 which allows a branch migration over ten nucleotides. The pair of opposite strands are 01/0.3 and 02/04 in CFK1a01 and 11/13 and 12/14 in CFK1a11. CFK1a11 is cleaved at identical positions, three nucleotides 3' of the junction. Strong bias for cleavage axis 12/14 with more than 80% of the substrate being cleaved, while only less than 10% along axis 11713 is cleaved. CFK1a01 is cleaved along both axes with equal efficiency, cleavage positions | Pyrococcus abyssi | ? | - |
? | |
| 3.1.21.10 | DNA + H2O | cleaves the Holliday junction in a symmetrical mode by introducing two nicks in opposite strands across the junction. Cleavage of fixed cruciform DNA CFK1a11 and CFK1a01 which allows a branch migration over ten nucleotides. The pair of opposite strands are 01/0.3 and 02/04 in CFK1a01 and 11/13 and 12/14 in CFK1a11. CFK1a11 is cleaved at identical positions, three nucleotides 3' of the junction. Strong bias for cleavage axis 12/14 with more than 80% of the substrate being cleaved, while only less than 10% along axis 11713 is cleaved. CFK1a01 is cleaved along both axes with equal efficiency, cleavage positions | Aeropyrum pernix | ? | - |
? | |
| 3.1.21.10 | DNA + H2O | cleaves the Holliday junction in a symmetrical mode by introducing two nicks in opposite strands across the junction. Cleavage of fixed cruciform DNA CFK1a11 and CFK1a01 which allows a branch migration over ten nucleotides. The pair of opposite strands are 01/0.3 and 02/04 in CFK1a01 and 11/13 and 12/14 in CFK1a11. CFK1a11 is cleaved at identical positions, three nucleotides 3' of the junction. Strong bias for cleavage axis 12/14 with more than 80% of the substrate being cleaved, while only less than 10% along axis 11713 is cleaved. CFK1a01 is cleaved more efficiently along the 01/04 axis, cleavage positions | Methanocaldococcus jannaschii | ? | - |
? | |
| 3.1.21.10 | DNA + H2O | cleaves the Holliday junction in a symmetrical mode by introducing two nicks in opposite strands across the junction. Cleavage of fixed cruciform DNA CFK1a11 and CFK1a01 which allows a branch migration over ten nucleotides. The pair of opposite strands are 01/0.3 and 02/04 in CFK1a01 and 11/13 and 12/14 in CFK1a11. CFK1a11 is cleaved at identical positions, three nucleotides 3' of the junction. Strong bias for cleavage axis 12/14 with more than 80% of the substrate being cleaved, while only less than 10% along axis 11713 is cleaved. For the substrate CFK1a01 the enzyme has a strong bias for the 01/03 axis, cleavage positions | Archaeoglobus fulgidus | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.1.21.10 | Afu-Hjc | - |
Archaeoglobus fulgidus |
| 3.1.21.10 | Ape-Hjc | - |
Aeropyrum pernix |
| 3.1.21.10 | Holliday junction-resolving enzymes | - |
Methanothermobacter thermautotrophicus |
| 3.1.21.10 | Holliday junction-resolving enzymes | - |
Methanocaldococcus jannaschii |
| 3.1.21.10 | Holliday junction-resolving enzymes | - |
Archaeoglobus fulgidus |
| 3.1.21.10 | Holliday junction-resolving enzymes | - |
Pyrococcus horikoshii |
| 3.1.21.10 | Holliday junction-resolving enzymes | - |
Pyrococcus abyssi |
| 3.1.21.10 | Holliday junction-resolving enzymes | - |
Aeropyrum pernix |
| 3.1.21.10 | Mja-Hjc | - |
Methanocaldococcus jannaschii |
| 3.1.21.10 | Mth-Hjc | - |
Methanothermobacter thermautotrophicus |
| 3.1.21.10 | Pab-Hjc | - |
Pyrococcus abyssi |
| 3.1.21.10 | Pho-Hjc | - |
Pyrococcus horikoshii |
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Release 2023.1 (January 2023)
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