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Literature summary extracted from

  • Bruton, G.; Huxley, A.; O'Hanlon, P.; Orlek, B.; Eggleston, D.; Humphries, J.; Readshaw, S.; West, A.; Ashman, S.; Brown, M.; Moore, K.; Pope, A.; O'Dwyer, K.; Wang, L.
    Lipopeptide substrates for SpsB, the Staphylococcus aureus type I signal peptidase: design, conformation and conversion to alpha-ketoamide inhibitors (2003), Eur. J. Med. Chem., 38, 351-356.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.4.21.89 pharmacology the gram positive pathogen plays a significant role in infectious disease, including life threatening methicillin-resistant MRSA infections, the ability to screen for inhibitors of SpsB will represent a significant advance for discovery of antibacterial agents Staphylococcus aureus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.89 decanoyl-LTPTA susceptible to classic protease inhibition, SpsB can be inhibited with a P1' proline Staphylococcus aureus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.21.89 0.029
-
 decanoyl-LTPTAKAASKIDD-OH pH 8.5, 37°C Staphylococcus aureus
3.4.21.89 0.988
-
 decanoyl-LT-P-TAKA-A-SKIDD-OH pH 8.5, 37°C Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.21.89 signal peptides from preproteins + H2O Staphylococcus aureus
-
 mature proteins
-
 ?
3.4.21.89 signal peptides from preproteins + H2O Escherichia coli
-
 mature proteins
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.89 Escherichia coli
-
-
-
3.4.21.89 Staphylococcus aureus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.89 decanoyl-LTPTAKAASKIDD-OH + H2O
-
 Staphylococcus aureus decanoyl-LTPTAKA + ASKIDD
-
 ?
3.4.21.89 decanoyl-LTPTAKAASKIDD-OH + H2O
-
 Escherichia coli decanoyl-LTPTAKA + ASKIDD
-
 ?
3.4.21.89 hexanoyl-LTPTQAKAASKIDD-OH + H2O
-
 Staphylococcus aureus hexanoyl-LTPTQAKA + ASKIDD
-
 ?
3.4.21.89 octanoyl-LTPTQAKAASKIDD-OH + H2O
-
 Staphylococcus aureus octanoyl-LTPTQAKA + ASKIDD
-
 ?
3.4.21.89 pro-ompA-nuclease + H2O
-
 Staphylococcus aureus ompA-nuclease + ?
-
 ?
3.4.21.89 pro-ompA-nuclease + H2O
-
 Escherichia coli ompA-nuclease + ?
-
 ?
3.4.21.89 signal peptides from preproteins + H2O
-
 Staphylococcus aureus mature proteins
-
 ?
3.4.21.89 signal peptides from preproteins + H2O
-
 Escherichia coli mature proteins
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.21.89 SpsB
-
 Staphylococcus aureus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.21.89 67
-
 decanoyl-LTPTAKAASKIDD-OH pH 8.5, 37°C Staphylococcus aureus
3.4.21.89 418
-
 decanoyl-LTPTAKAASKIDD-OH pH 8.5, 37°C Staphylococcus aureus