BRENDA - Enzyme Database

Characterization of acetyl-CoA/propionyl-CoA carboxylase in Metallosphaera sedula. Carboxylating enzyme in the 3-hydroxypropionate cycle for autotrophic carbon fixation

Hugler, M.; Krieger, R.S.; Jahn, M.; Fuchs, G.; Eur. J. Biochem. 270, 736-744 (2003)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Cloned (Commentary)
Organism
6.4.1.2
each subunit individually cloned, carboxyl transferase expressed in Escherichia coli
Metallosphaera sedula
6.4.1.3
expressed in Escherichia coli
Metallosphaera sedula
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
6.4.1.2
ADP
-
Metallosphaera sedula
6.4.1.2
Avidin
reversible by addition of biotin
Metallosphaera sedula
6.4.1.2
CoA
-
Metallosphaera sedula
6.4.1.2
malonyl-CoA
-
Metallosphaera sedula
6.4.1.2
succinyl-CoA
-
Metallosphaera sedula
6.4.1.3
ADP
weak inhibition
Metallosphaera sedula
6.4.1.3
CoA
activity on propanoyl-CoA is more strongly inhibited than activity of acetyl-CoA
Metallosphaera sedula
6.4.1.3
malonyl-CoA
activity on propanoyl-CoA is more strongly inhibited than activity of acetyl-CoA
Metallosphaera sedula
6.4.1.3
succinyl-CoA
activity on propanoyl-CoA is more strongly inhibited than activity of acetyl-CoA
Metallosphaera sedula
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
6.4.1.2
0.04
-
ATP
pH 7.5, 65°C
Metallosphaera sedula
6.4.1.2
0.06
-
acetyl-CoA
pH 7.5, 65°C
Metallosphaera sedula
6.4.1.2
0.3
-
HCO3-
pH 7.5, 65°C
Metallosphaera sedula
6.4.1.3
0.04
-
ATP
pH 7.5, 65°C
Metallosphaera sedula
6.4.1.3
0.06
-
acetyl-CoA
pH 7.5, 65°C
Metallosphaera sedula
6.4.1.3
0.07
-
propanoyl-CoA
pH 7.5, 65°C
Metallosphaera sedula
6.4.1.3
0.3
-
HCO3-
pH 7.5, 65°C
Metallosphaera sedula
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
6.4.1.2
Ca2+
can partially replace for Mg2+
Metallosphaera sedula
6.4.1.2
Co2+
can partially replace for Mg2+
Metallosphaera sedula
6.4.1.2
Mg2+
required to form a complex with ATP
Metallosphaera sedula
6.4.1.2
Mn2+
can partially replace for Mg2+
Metallosphaera sedula
6.4.1.3
Ca2+
10% of the activity with Mg2+
Metallosphaera sedula
6.4.1.3
Co2+
40% of the activity with Mg2+
Metallosphaera sedula
6.4.1.3
Mg2+
required for the formation of Mg-ATP complex, optimum concentration 5 mM at 5 mM ATP
Metallosphaera sedula
6.4.1.3
Mn2+
10% of the activity with Mg2+
Metallosphaera sedula
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
6.4.1.2
560000
-
gel filtration
Metallosphaera sedula
6.4.1.3
18600
-
alpha, beta, gamma, 4 * 57000 + 4 * 57000 + 4 * 18600, SDS-PAGE, biotin carboxylase (alpha subunit), carboxyl transferase (beta subunit) and biotin carrier protein (gamma subunit)
Metallosphaera sedula
6.4.1.3
57000
-
alpha, beta, gamma, 4 * 57000 + 4 * 57000 + 4 * 18600, SDS-PAGE, biotin carboxylase (alpha subunit), carboxyl transferase (beta subunit) and biotin carrier protein (gamma subunit)
Metallosphaera sedula
6.4.1.3
560000
-
gel filtration
Metallosphaera sedula
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
6.4.1.2
ATP + acetyl-CoA + HCO3-
Chloroflexus aurantiacus
no fatty acid synthesis, involved in 3-hydroxypropionate cycle for autotrophic CO2 fixation
ADP + phosphate + malonyl-CoA
-
Chloroflexus aurantiacus
?
6.4.1.2
ATP + acetyl-CoA + HCO3-
Sulfuracidifex metallicus
no fatty acid synthesis, involved in 3-hydroxypropionate cycle for autotrophic CO2 fixation
ADP + phosphate + malonyl-CoA
-
Sulfuracidifex metallicus
?
6.4.1.2
ATP + acetyl-CoA + HCO3-
Metallosphaera sedula
no fatty acid synthesis, involved in 3-hydroxypropionate cycle for autotrophic CO2 fixation
ADP + phosphate + malonyl-CoA
-
Metallosphaera sedula
?
6.4.1.2
ATP + acetyl-CoA + HCO3-
Metallosphaera sedula TH2 / ATCC 51363 / DSM 5348
no fatty acid synthesis, involved in 3-hydroxypropionate cycle for autotrophic CO2 fixation
ADP + phosphate + malonyl-CoA
-
Metallosphaera sedula TH2 / ATCC 51363 / DSM 5348
?
6.4.1.2
ATP + acetyl-CoA + HCO3-
Chloroflexus aurantiacus OK-70-fl / DSM 636
no fatty acid synthesis, involved in 3-hydroxypropionate cycle for autotrophic CO2 fixation
ADP + phosphate + malonyl-CoA
-
Chloroflexus aurantiacus OK-70-fl / DSM 636
?
6.4.1.3
ATP + propanoyl-CoA + HCO3-
Chloroflexus aurantiacus
CO2 fixation during autotrophic growth, 3 -hydroxypropionate cycle
ADP + phosphate + (S)-methylmalonyl-CoA
-
Chloroflexus aurantiacus
?
6.4.1.3
ATP + propanoyl-CoA + HCO3-
Metallosphaera sedula
CO2 fixation during autotrophic growth, 3 -hydroxypropionate cycle
ADP + phosphate + (S)-methylmalonyl-CoA
-
Metallosphaera sedula
?
6.4.1.3
ATP + propanoyl-CoA + HCO3-
Sulfuracidifex metallicus
CO2 fixation during autotrophic growth, 3 -hydroxypropionate cycle
ADP + phosphate + (S)-methylmalonyl-CoA
-
Sulfuracidifex metallicus
?
6.4.1.3
ATP + propanoyl-CoA + HCO3-
Metallosphaera sedula TH2 / ATCC 51363 / DSM 5348
CO2 fixation during autotrophic growth, 3 -hydroxypropionate cycle
ADP + phosphate + (S)-methylmalonyl-CoA
-
Metallosphaera sedula TH2 / ATCC 51363 / DSM 5348
?
6.4.1.3
ATP + propanoyl-CoA + HCO3-
Chloroflexus aurantiacus OK-70-fl / DSM 636
CO2 fixation during autotrophic growth, 3 -hydroxypropionate cycle
ADP + phosphate + (S)-methylmalonyl-CoA
-
Chloroflexus aurantiacus OK-70-fl / DSM 636
?
Organism
EC Number
Organism
UniProt
Commentary
Textmining
6.4.1.2
Chloroflexus aurantiacus
-
-
-
6.4.1.2
Chloroflexus aurantiacus OK-70-fl / DSM 636
-
-
-
6.4.1.2
Metallosphaera sedula
Q8J2Z4
-
-
6.4.1.2
Metallosphaera sedula
Q8J2Z5
-
-
6.4.1.2
Metallosphaera sedula TH2 / ATCC 51363 / DSM 5348
Q8J2Z4
-
-
6.4.1.2
Metallosphaera sedula TH2 / ATCC 51363 / DSM 5348
Q8J2Z5
-
-
6.4.1.2
Sulfuracidifex metallicus
-
DSM 6482
-
6.4.1.3
Chloroflexus aurantiacus
-
-
-
6.4.1.3
Chloroflexus aurantiacus OK-70-fl / DSM 636
-
-
-
6.4.1.3
Metallosphaera sedula
-
-
-
6.4.1.3
Metallosphaera sedula TH2 / ATCC 51363 / DSM 5348
-
-
-
6.4.1.3
Sulfuracidifex metallicus
-
DSM 6482
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
6.4.1.2
-
Metallosphaera sedula
6.4.1.2
partial
Chloroflexus aurantiacus
6.4.1.3
-
Metallosphaera sedula
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
6.4.1.2
3.2
-
purified enzyme, pH 7.5, 65°C
Metallosphaera sedula
6.4.1.3
3.3
-
purified enzyme, pH 7.5, 65°C
Metallosphaera sedula
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
6.4.1.2
ATP + acetyl-CoA + HCO3-
-
651169
Chloroflexus aurantiacus
ADP + phosphate + malonyl-CoA
-
651169
Chloroflexus aurantiacus
?
6.4.1.2
ATP + acetyl-CoA + HCO3-
-
651169
Metallosphaera sedula
ADP + phosphate + malonyl-CoA
-
-
-
?
6.4.1.2
ATP + acetyl-CoA + HCO3-
-
651169
Sulfuracidifex metallicus
ADP + phosphate + malonyl-CoA
-
651169
Sulfuracidifex metallicus
?
6.4.1.2
ATP + acetyl-CoA + HCO3-
-
651169
Metallosphaera sedula
ADP + phosphate + malonyl-CoA
-
651169
Metallosphaera sedula
?
6.4.1.2
ATP + acetyl-CoA + HCO3-
no fatty acid synthesis, involved in 3-hydroxypropionate cycle for autotrophic CO2 fixation
651169
Chloroflexus aurantiacus
ADP + phosphate + malonyl-CoA
-
651169
Chloroflexus aurantiacus
?
6.4.1.2
ATP + acetyl-CoA + HCO3-
no fatty acid synthesis, involved in 3-hydroxypropionate cycle for autotrophic CO2 fixation
651169
Sulfuracidifex metallicus
ADP + phosphate + malonyl-CoA
-
651169
Sulfuracidifex metallicus
?
6.4.1.2
ATP + acetyl-CoA + HCO3-
no fatty acid synthesis, involved in 3-hydroxypropionate cycle for autotrophic CO2 fixation
651169
Metallosphaera sedula
ADP + phosphate + malonyl-CoA
-
651169
Metallosphaera sedula
?
6.4.1.2
ATP + acetyl-CoA + HCO3-
no fatty acid synthesis, involved in 3-hydroxypropionate cycle for autotrophic CO2 fixation
651169
Metallosphaera sedula TH2 / ATCC 51363 / DSM 5348
ADP + phosphate + malonyl-CoA
-
651169
Metallosphaera sedula TH2 / ATCC 51363 / DSM 5348
?
6.4.1.2
ATP + acetyl-CoA + HCO3-
-
651169
Metallosphaera sedula TH2 / ATCC 51363 / DSM 5348
ADP + phosphate + malonyl-CoA
-
651169
Metallosphaera sedula TH2 / ATCC 51363 / DSM 5348
?
6.4.1.2
ATP + acetyl-CoA + HCO3-
-
651169
Chloroflexus aurantiacus OK-70-fl / DSM 636
ADP + phosphate + malonyl-CoA
-
651169
Chloroflexus aurantiacus OK-70-fl / DSM 636
?
6.4.1.2
ATP + acetyl-CoA + HCO3-
no fatty acid synthesis, involved in 3-hydroxypropionate cycle for autotrophic CO2 fixation
651169
Chloroflexus aurantiacus OK-70-fl / DSM 636
ADP + phosphate + malonyl-CoA
-
651169
Chloroflexus aurantiacus OK-70-fl / DSM 636
?
6.4.1.2
ATP + propionyl-CoA + HCO3-
second activity of the protein representing EC 6.4.1.3
651169
Metallosphaera sedula
ADP + phosphate + methylmalonyl-CoA
-
651169
Metallosphaera sedula
?
6.4.1.2
ATP + propionyl-CoA + HCO3-
second activity of the protein representing EC 6.4.1.3
651169
Metallosphaera sedula TH2 / ATCC 51363 / DSM 5348
ADP + phosphate + methylmalonyl-CoA
-
651169
Metallosphaera sedula TH2 / ATCC 51363 / DSM 5348
?
6.4.1.2
GTP + acetyl-CoA + HCO3-
10% of the activity obtained with ATP
651169
Metallosphaera sedula
GDP + phosphate + malonyl-CoA
-
651169
Metallosphaera sedula
?
6.4.1.2
GTP + acetyl-CoA + HCO3-
10% of the activity obtained with ATP
651169
Metallosphaera sedula TH2 / ATCC 51363 / DSM 5348
GDP + phosphate + malonyl-CoA
-
651169
Metallosphaera sedula TH2 / ATCC 51363 / DSM 5348
?
6.4.1.2
UTP + acetyl-CoA + HCO3-
40% of the activity obtained with ATP
651169
Metallosphaera sedula
UDP + phosphate + malonyl-CoA
-
651169
Metallosphaera sedula
?
6.4.1.2
UTP + acetyl-CoA + HCO3-
40% of the activity obtained with ATP
651169
Metallosphaera sedula TH2 / ATCC 51363 / DSM 5348
UDP + phosphate + malonyl-CoA
-
651169
Metallosphaera sedula TH2 / ATCC 51363 / DSM 5348
?
6.4.1.3
ATP + acetyl-CoA + HCO3-
EC 6.4.1.2 activity
651169
Chloroflexus aurantiacus
ADP + phosphate + malonyl-CoA
-
651169
Chloroflexus aurantiacus
?
6.4.1.3
ATP + acetyl-CoA + HCO3-
EC 6.4.1.2 activity
651169
Metallosphaera sedula
ADP + phosphate + malonyl-CoA
-
651169
Metallosphaera sedula
?
6.4.1.3
ATP + acetyl-CoA + HCO3-
EC 6.4.1.2 activity
651169
Sulfuracidifex metallicus
ADP + phosphate + malonyl-CoA
-
651169
Sulfuracidifex metallicus
?
6.4.1.3
ATP + acetyl-CoA + HCO3-
EC 6.4.1.2 activity
651169
Metallosphaera sedula TH2 / ATCC 51363 / DSM 5348
ADP + phosphate + malonyl-CoA
-
651169
Metallosphaera sedula TH2 / ATCC 51363 / DSM 5348
?
6.4.1.3
ATP + acetyl-CoA + HCO3-
EC 6.4.1.2 activity
651169
Chloroflexus aurantiacus OK-70-fl / DSM 636
ADP + phosphate + malonyl-CoA
-
651169
Chloroflexus aurantiacus OK-70-fl / DSM 636
?
6.4.1.3
ATP + propanoyl-CoA + HCO3-
-
651169
Chloroflexus aurantiacus
ADP + phosphate + (S)-methylmalonyl-CoA
-
651169
Chloroflexus aurantiacus
?
6.4.1.3
ATP + propanoyl-CoA + HCO3-
-
651169
Metallosphaera sedula
ADP + phosphate + (S)-methylmalonyl-CoA
-
651169
Metallosphaera sedula
?
6.4.1.3
ATP + propanoyl-CoA + HCO3-
-
651169
Sulfuracidifex metallicus
ADP + phosphate + (S)-methylmalonyl-CoA
-
651169
Sulfuracidifex metallicus
?
6.4.1.3
ATP + propanoyl-CoA + HCO3-
CO2 fixation during autotrophic growth, 3 -hydroxypropionate cycle
651169
Chloroflexus aurantiacus
ADP + phosphate + (S)-methylmalonyl-CoA
-
651169
Chloroflexus aurantiacus
?
6.4.1.3
ATP + propanoyl-CoA + HCO3-
CO2 fixation during autotrophic growth, 3 -hydroxypropionate cycle
651169
Metallosphaera sedula
ADP + phosphate + (S)-methylmalonyl-CoA
-
651169
Metallosphaera sedula
?
6.4.1.3
ATP + propanoyl-CoA + HCO3-
CO2 fixation during autotrophic growth, 3 -hydroxypropionate cycle
651169
Sulfuracidifex metallicus
ADP + phosphate + (S)-methylmalonyl-CoA
-
651169
Sulfuracidifex metallicus
?
6.4.1.3
ATP + propanoyl-CoA + HCO3-
-
651169
Metallosphaera sedula TH2 / ATCC 51363 / DSM 5348
ADP + phosphate + (S)-methylmalonyl-CoA
-
651169
Metallosphaera sedula TH2 / ATCC 51363 / DSM 5348
?
6.4.1.3
ATP + propanoyl-CoA + HCO3-
CO2 fixation during autotrophic growth, 3 -hydroxypropionate cycle
651169
Metallosphaera sedula TH2 / ATCC 51363 / DSM 5348
ADP + phosphate + (S)-methylmalonyl-CoA
-
651169
Metallosphaera sedula TH2 / ATCC 51363 / DSM 5348
?
6.4.1.3
ATP + propanoyl-CoA + HCO3-
-
651169
Chloroflexus aurantiacus OK-70-fl / DSM 636
ADP + phosphate + (S)-methylmalonyl-CoA
-
651169
Chloroflexus aurantiacus OK-70-fl / DSM 636
?
6.4.1.3
ATP + propanoyl-CoA + HCO3-
CO2 fixation during autotrophic growth, 3 -hydroxypropionate cycle
651169
Chloroflexus aurantiacus OK-70-fl / DSM 636
ADP + phosphate + (S)-methylmalonyl-CoA
-
651169
Chloroflexus aurantiacus OK-70-fl / DSM 636
?
6.4.1.3
GTP + propanoyl-CoA + HCO3-
10% of the activity with ATP
651169
Metallosphaera sedula
GDP + phosphate + (S)-methylmalonyl-CoA
-
651169
Metallosphaera sedula
?
6.4.1.3
GTP + propanoyl-CoA + HCO3-
10% of the activity with ATP
651169
Metallosphaera sedula TH2 / ATCC 51363 / DSM 5348
GDP + phosphate + (S)-methylmalonyl-CoA
-
651169
Metallosphaera sedula TH2 / ATCC 51363 / DSM 5348
?
6.4.1.3
UTP + propanoyl-CoA + HCO3-
40% of the activity with ATP
651169
Metallosphaera sedula
UDP + phosphate + (S)-methylmalonyl-CoA
-
651169
Metallosphaera sedula
?
6.4.1.3
UTP + propanoyl-CoA + HCO3-
40% of the activity with ATP
651169
Metallosphaera sedula TH2 / ATCC 51363 / DSM 5348
UDP + phosphate + (S)-methylmalonyl-CoA
-
651169
Metallosphaera sedula TH2 / ATCC 51363 / DSM 5348
?
Subunits
EC Number
Subunits
Commentary
Organism
6.4.1.2
multimer
most likely (alpha,beta,gamma)4 composition, two large subunits of 57000 Da: biotin carboxylase (alpha subunit) and carboxyl transferase (gamma subunit), 18600 Da biotin carrier protein (beta subunit)
Chloroflexus aurantiacus
6.4.1.2
multimer
most likely (alpha,beta,gamma)4 composition, two large subunits of 57000 Da: biotin carboxylase (alpha subunit) and carboxyl transferase (gamma subunit), 18600 Da biotin carrier protein (beta subunit)
Sulfuracidifex metallicus
6.4.1.2
multimer
most likely (alpha,beta,gamma)4 composition, two large subunits of 57000 Da: biotin carboxylase (alpha subunit) and carboxyl transferase (gamma subunit), 18600 Da biotin carrier protein (beta subunit)
Metallosphaera sedula
6.4.1.3
dodecamer
alpha, beta, gamma, 4 * 57000 + 4 * 57000 + 4 * 18600, SDS-PAGE, biotin carboxylase (alpha subunit), carboxyl transferase (beta subunit) and biotin carrier protein (gamma subunit)
Metallosphaera sedula
Synonyms
EC Number
Synonyms
Commentary
Organism
6.4.1.2
acetyl-CoA/propionyl-CoA carboxylase
enzyme acts on both substrates
Chloroflexus aurantiacus
6.4.1.2
acetyl-CoA/propionyl-CoA carboxylase
enzyme acts on both substrates
Sulfuracidifex metallicus
6.4.1.2
acetyl-CoA/propionyl-CoA carboxylase
enzyme acts on both substrates
Metallosphaera sedula
6.4.1.3
acetyl-CoA/propionyl-CoA carboxylase
enzyme acts equally well on both substrates
Chloroflexus aurantiacus
6.4.1.3
acetyl-CoA/propionyl-CoA carboxylase
enzyme acts equally well on both substrates
Metallosphaera sedula
6.4.1.3
acetyl-CoA/propionyl-CoA carboxylase
enzyme acts equally well on both substrates
Sulfuracidifex metallicus
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
6.4.1.2
75
-
-
Metallosphaera sedula
6.4.1.3
75
-
-
Metallosphaera sedula
Temperature Range [°C]
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
6.4.1.2
40
85
-
Metallosphaera sedula
6.4.1.3
40
85
-
Metallosphaera sedula
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
6.4.1.2
2
8
acetyl-CoA
pH 7.5, 65°C
Metallosphaera sedula
6.4.1.3
2
8
propanoyl-CoA
pH 7.5, 65°C
Metallosphaera sedula
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.4.1.2
7.5
-
-
Metallosphaera sedula
6.4.1.3
7.5
-
-
Metallosphaera sedula
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
6.4.1.2
6
8
-
Metallosphaera sedula
6.4.1.3
6
8.5
-
Metallosphaera sedula
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
6.4.1.2
biotin
enzyme-bound
Chloroflexus aurantiacus
6.4.1.2
biotin
enzyme-bound
Sulfuracidifex metallicus
6.4.1.2
biotin
enzyme-bound
Metallosphaera sedula
6.4.1.3
biotin
bound to enzyme
Chloroflexus aurantiacus
6.4.1.3
biotin
bound to enzyme
Metallosphaera sedula
6.4.1.3
biotin
bound to enzyme
Sulfuracidifex metallicus
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
6.4.1.2
each subunit individually cloned, carboxyl transferase expressed in Escherichia coli
Metallosphaera sedula
6.4.1.3
expressed in Escherichia coli
Metallosphaera sedula
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
6.4.1.2
biotin
enzyme-bound
Chloroflexus aurantiacus
6.4.1.2
biotin
enzyme-bound
Sulfuracidifex metallicus
6.4.1.2
biotin
enzyme-bound
Metallosphaera sedula
6.4.1.3
biotin
bound to enzyme
Chloroflexus aurantiacus
6.4.1.3
biotin
bound to enzyme
Metallosphaera sedula
6.4.1.3
biotin
bound to enzyme
Sulfuracidifex metallicus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
6.4.1.2
ADP
-
Metallosphaera sedula
6.4.1.2
Avidin
reversible by addition of biotin
Metallosphaera sedula
6.4.1.2
CoA
-
Metallosphaera sedula
6.4.1.2
malonyl-CoA
-
Metallosphaera sedula
6.4.1.2
succinyl-CoA
-
Metallosphaera sedula
6.4.1.3
ADP
weak inhibition
Metallosphaera sedula
6.4.1.3
CoA
activity on propanoyl-CoA is more strongly inhibited than activity of acetyl-CoA
Metallosphaera sedula
6.4.1.3
malonyl-CoA
activity on propanoyl-CoA is more strongly inhibited than activity of acetyl-CoA
Metallosphaera sedula
6.4.1.3
succinyl-CoA
activity on propanoyl-CoA is more strongly inhibited than activity of acetyl-CoA
Metallosphaera sedula
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
6.4.1.2
0.04
-
ATP
pH 7.5, 65°C
Metallosphaera sedula
6.4.1.2
0.06
-
acetyl-CoA
pH 7.5, 65°C
Metallosphaera sedula
6.4.1.2
0.3
-
HCO3-
pH 7.5, 65°C
Metallosphaera sedula
6.4.1.3
0.04
-
ATP
pH 7.5, 65°C
Metallosphaera sedula
6.4.1.3
0.06
-
acetyl-CoA
pH 7.5, 65°C
Metallosphaera sedula
6.4.1.3
0.07
-
propanoyl-CoA
pH 7.5, 65°C
Metallosphaera sedula
6.4.1.3
0.3
-
HCO3-
pH 7.5, 65°C
Metallosphaera sedula
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
6.4.1.2
Ca2+
can partially replace for Mg2+
Metallosphaera sedula
6.4.1.2
Co2+
can partially replace for Mg2+
Metallosphaera sedula
6.4.1.2
Mg2+
required to form a complex with ATP
Metallosphaera sedula
6.4.1.2
Mn2+
can partially replace for Mg2+
Metallosphaera sedula
6.4.1.3
Ca2+
10% of the activity with Mg2+
Metallosphaera sedula
6.4.1.3
Co2+
40% of the activity with Mg2+
Metallosphaera sedula
6.4.1.3
Mg2+
required for the formation of Mg-ATP complex, optimum concentration 5 mM at 5 mM ATP
Metallosphaera sedula
6.4.1.3
Mn2+
10% of the activity with Mg2+
Metallosphaera sedula
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
6.4.1.2
560000
-
gel filtration
Metallosphaera sedula
6.4.1.3
18600
-
alpha, beta, gamma, 4 * 57000 + 4 * 57000 + 4 * 18600, SDS-PAGE, biotin carboxylase (alpha subunit), carboxyl transferase (beta subunit) and biotin carrier protein (gamma subunit)
Metallosphaera sedula
6.4.1.3
57000
-
alpha, beta, gamma, 4 * 57000 + 4 * 57000 + 4 * 18600, SDS-PAGE, biotin carboxylase (alpha subunit), carboxyl transferase (beta subunit) and biotin carrier protein (gamma subunit)
Metallosphaera sedula
6.4.1.3
560000
-
gel filtration
Metallosphaera sedula
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
6.4.1.2
ATP + acetyl-CoA + HCO3-
Chloroflexus aurantiacus
no fatty acid synthesis, involved in 3-hydroxypropionate cycle for autotrophic CO2 fixation
ADP + phosphate + malonyl-CoA
-
Chloroflexus aurantiacus
?
6.4.1.2
ATP + acetyl-CoA + HCO3-
Sulfuracidifex metallicus
no fatty acid synthesis, involved in 3-hydroxypropionate cycle for autotrophic CO2 fixation
ADP + phosphate + malonyl-CoA
-
Sulfuracidifex metallicus
?
6.4.1.2
ATP + acetyl-CoA + HCO3-
Metallosphaera sedula
no fatty acid synthesis, involved in 3-hydroxypropionate cycle for autotrophic CO2 fixation
ADP + phosphate + malonyl-CoA
-
Metallosphaera sedula
?
6.4.1.2
ATP + acetyl-CoA + HCO3-
Metallosphaera sedula TH2 / ATCC 51363 / DSM 5348
no fatty acid synthesis, involved in 3-hydroxypropionate cycle for autotrophic CO2 fixation
ADP + phosphate + malonyl-CoA
-
Metallosphaera sedula TH2 / ATCC 51363 / DSM 5348
?
6.4.1.2
ATP + acetyl-CoA + HCO3-
Chloroflexus aurantiacus OK-70-fl / DSM 636
no fatty acid synthesis, involved in 3-hydroxypropionate cycle for autotrophic CO2 fixation
ADP + phosphate + malonyl-CoA
-
Chloroflexus aurantiacus OK-70-fl / DSM 636
?
6.4.1.3
ATP + propanoyl-CoA + HCO3-
Chloroflexus aurantiacus
CO2 fixation during autotrophic growth, 3 -hydroxypropionate cycle
ADP + phosphate + (S)-methylmalonyl-CoA
-
Chloroflexus aurantiacus
?
6.4.1.3
ATP + propanoyl-CoA + HCO3-
Metallosphaera sedula
CO2 fixation during autotrophic growth, 3 -hydroxypropionate cycle
ADP + phosphate + (S)-methylmalonyl-CoA
-
Metallosphaera sedula
?
6.4.1.3
ATP + propanoyl-CoA + HCO3-
Sulfuracidifex metallicus
CO2 fixation during autotrophic growth, 3 -hydroxypropionate cycle
ADP + phosphate + (S)-methylmalonyl-CoA
-
Sulfuracidifex metallicus
?
6.4.1.3
ATP + propanoyl-CoA + HCO3-
Metallosphaera sedula TH2 / ATCC 51363 / DSM 5348
CO2 fixation during autotrophic growth, 3 -hydroxypropionate cycle
ADP + phosphate + (S)-methylmalonyl-CoA
-
Metallosphaera sedula TH2 / ATCC 51363 / DSM 5348
?
6.4.1.3
ATP + propanoyl-CoA + HCO3-
Chloroflexus aurantiacus OK-70-fl / DSM 636
CO2 fixation during autotrophic growth, 3 -hydroxypropionate cycle
ADP + phosphate + (S)-methylmalonyl-CoA
-
Chloroflexus aurantiacus OK-70-fl / DSM 636
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
6.4.1.2
-
Metallosphaera sedula
6.4.1.2
partial
Chloroflexus aurantiacus
6.4.1.3
-
Metallosphaera sedula
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
6.4.1.2
3.2
-
purified enzyme, pH 7.5, 65°C
Metallosphaera sedula
6.4.1.3
3.3
-
purified enzyme, pH 7.5, 65°C
Metallosphaera sedula
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
6.4.1.2
ATP + acetyl-CoA + HCO3-
-
651169
Chloroflexus aurantiacus
ADP + phosphate + malonyl-CoA
-
651169
Chloroflexus aurantiacus
?
6.4.1.2
ATP + acetyl-CoA + HCO3-
-
651169
Metallosphaera sedula
ADP + phosphate + malonyl-CoA
-
-
-
?
6.4.1.2
ATP + acetyl-CoA + HCO3-
-
651169
Sulfuracidifex metallicus
ADP + phosphate + malonyl-CoA
-
651169
Sulfuracidifex metallicus
?
6.4.1.2
ATP + acetyl-CoA + HCO3-
-
651169
Metallosphaera sedula
ADP + phosphate + malonyl-CoA
-
651169
Metallosphaera sedula
?
6.4.1.2
ATP + acetyl-CoA + HCO3-
no fatty acid synthesis, involved in 3-hydroxypropionate cycle for autotrophic CO2 fixation
651169
Chloroflexus aurantiacus
ADP + phosphate + malonyl-CoA
-
651169
Chloroflexus aurantiacus
?
6.4.1.2
ATP + acetyl-CoA + HCO3-
no fatty acid synthesis, involved in 3-hydroxypropionate cycle for autotrophic CO2 fixation
651169
Sulfuracidifex metallicus
ADP + phosphate + malonyl-CoA
-
651169
Sulfuracidifex metallicus
?
6.4.1.2
ATP + acetyl-CoA + HCO3-
no fatty acid synthesis, involved in 3-hydroxypropionate cycle for autotrophic CO2 fixation
651169
Metallosphaera sedula
ADP + phosphate + malonyl-CoA
-
651169
Metallosphaera sedula
?
6.4.1.2
ATP + acetyl-CoA + HCO3-
no fatty acid synthesis, involved in 3-hydroxypropionate cycle for autotrophic CO2 fixation
651169
Metallosphaera sedula TH2 / ATCC 51363 / DSM 5348
ADP + phosphate + malonyl-CoA
-
651169
Metallosphaera sedula TH2 / ATCC 51363 / DSM 5348
?
6.4.1.2
ATP + acetyl-CoA + HCO3-
-
651169
Metallosphaera sedula TH2 / ATCC 51363 / DSM 5348
ADP + phosphate + malonyl-CoA
-
651169
Metallosphaera sedula TH2 / ATCC 51363 / DSM 5348
?
6.4.1.2
ATP + acetyl-CoA + HCO3-
-
651169
Chloroflexus aurantiacus OK-70-fl / DSM 636
ADP + phosphate + malonyl-CoA
-
651169
Chloroflexus aurantiacus OK-70-fl / DSM 636
?
6.4.1.2
ATP + acetyl-CoA + HCO3-
no fatty acid synthesis, involved in 3-hydroxypropionate cycle for autotrophic CO2 fixation
651169
Chloroflexus aurantiacus OK-70-fl / DSM 636
ADP + phosphate + malonyl-CoA
-
651169
Chloroflexus aurantiacus OK-70-fl / DSM 636
?
6.4.1.2
ATP + propionyl-CoA + HCO3-
second activity of the protein representing EC 6.4.1.3
651169
Metallosphaera sedula
ADP + phosphate + methylmalonyl-CoA
-
651169
Metallosphaera sedula
?
6.4.1.2
ATP + propionyl-CoA + HCO3-
second activity of the protein representing EC 6.4.1.3
651169
Metallosphaera sedula TH2 / ATCC 51363 / DSM 5348
ADP + phosphate + methylmalonyl-CoA
-
651169
Metallosphaera sedula TH2 / ATCC 51363 / DSM 5348
?
6.4.1.2
GTP + acetyl-CoA + HCO3-
10% of the activity obtained with ATP
651169
Metallosphaera sedula
GDP + phosphate + malonyl-CoA
-
651169
Metallosphaera sedula
?
6.4.1.2
GTP + acetyl-CoA + HCO3-
10% of the activity obtained with ATP
651169
Metallosphaera sedula TH2 / ATCC 51363 / DSM 5348
GDP + phosphate + malonyl-CoA
-
651169
Metallosphaera sedula TH2 / ATCC 51363 / DSM 5348
?
6.4.1.2
UTP + acetyl-CoA + HCO3-
40% of the activity obtained with ATP
651169
Metallosphaera sedula
UDP + phosphate + malonyl-CoA
-
651169
Metallosphaera sedula
?
6.4.1.2
UTP + acetyl-CoA + HCO3-
40% of the activity obtained with ATP
651169
Metallosphaera sedula TH2 / ATCC 51363 / DSM 5348
UDP + phosphate + malonyl-CoA
-
651169
Metallosphaera sedula TH2 / ATCC 51363 / DSM 5348
?
6.4.1.3
ATP + acetyl-CoA + HCO3-
EC 6.4.1.2 activity
651169
Chloroflexus aurantiacus
ADP + phosphate + malonyl-CoA
-
651169
Chloroflexus aurantiacus
?
6.4.1.3
ATP + acetyl-CoA + HCO3-
EC 6.4.1.2 activity
651169
Metallosphaera sedula
ADP + phosphate + malonyl-CoA
-
651169
Metallosphaera sedula
?
6.4.1.3
ATP + acetyl-CoA + HCO3-
EC 6.4.1.2 activity
651169
Sulfuracidifex metallicus
ADP + phosphate + malonyl-CoA
-
651169
Sulfuracidifex metallicus
?
6.4.1.3
ATP + acetyl-CoA + HCO3-
EC 6.4.1.2 activity
651169
Metallosphaera sedula TH2 / ATCC 51363 / DSM 5348
ADP + phosphate + malonyl-CoA
-
651169
Metallosphaera sedula TH2 / ATCC 51363 / DSM 5348
?
6.4.1.3
ATP + acetyl-CoA + HCO3-
EC 6.4.1.2 activity
651169
Chloroflexus aurantiacus OK-70-fl / DSM 636
ADP + phosphate + malonyl-CoA
-
651169
Chloroflexus aurantiacus OK-70-fl / DSM 636
?
6.4.1.3
ATP + propanoyl-CoA + HCO3-
-
651169
Chloroflexus aurantiacus
ADP + phosphate + (S)-methylmalonyl-CoA
-
651169
Chloroflexus aurantiacus
?
6.4.1.3
ATP + propanoyl-CoA + HCO3-
-
651169
Metallosphaera sedula
ADP + phosphate + (S)-methylmalonyl-CoA
-
651169
Metallosphaera sedula
?
6.4.1.3
ATP + propanoyl-CoA + HCO3-
-
651169
Sulfuracidifex metallicus
ADP + phosphate + (S)-methylmalonyl-CoA
-
651169
Sulfuracidifex metallicus
?
6.4.1.3
ATP + propanoyl-CoA + HCO3-
CO2 fixation during autotrophic growth, 3 -hydroxypropionate cycle
651169
Chloroflexus aurantiacus
ADP + phosphate + (S)-methylmalonyl-CoA
-
651169
Chloroflexus aurantiacus
?
6.4.1.3
ATP + propanoyl-CoA + HCO3-
CO2 fixation during autotrophic growth, 3 -hydroxypropionate cycle
651169
Metallosphaera sedula
ADP + phosphate + (S)-methylmalonyl-CoA
-
651169
Metallosphaera sedula
?
6.4.1.3
ATP + propanoyl-CoA + HCO3-
CO2 fixation during autotrophic growth, 3 -hydroxypropionate cycle
651169
Sulfuracidifex metallicus
ADP + phosphate + (S)-methylmalonyl-CoA
-
651169
Sulfuracidifex metallicus
?
6.4.1.3
ATP + propanoyl-CoA + HCO3-
-
651169
Metallosphaera sedula TH2 / ATCC 51363 / DSM 5348
ADP + phosphate + (S)-methylmalonyl-CoA
-
651169
Metallosphaera sedula TH2 / ATCC 51363 / DSM 5348
?
6.4.1.3
ATP + propanoyl-CoA + HCO3-
CO2 fixation during autotrophic growth, 3 -hydroxypropionate cycle
651169
Metallosphaera sedula TH2 / ATCC 51363 / DSM 5348
ADP + phosphate + (S)-methylmalonyl-CoA
-
651169
Metallosphaera sedula TH2 / ATCC 51363 / DSM 5348
?
6.4.1.3
ATP + propanoyl-CoA + HCO3-
-
651169
Chloroflexus aurantiacus OK-70-fl / DSM 636
ADP + phosphate + (S)-methylmalonyl-CoA
-
651169
Chloroflexus aurantiacus OK-70-fl / DSM 636
?
6.4.1.3
ATP + propanoyl-CoA + HCO3-
CO2 fixation during autotrophic growth, 3 -hydroxypropionate cycle
651169
Chloroflexus aurantiacus OK-70-fl / DSM 636
ADP + phosphate + (S)-methylmalonyl-CoA
-
651169
Chloroflexus aurantiacus OK-70-fl / DSM 636
?
6.4.1.3
GTP + propanoyl-CoA + HCO3-
10% of the activity with ATP
651169
Metallosphaera sedula
GDP + phosphate + (S)-methylmalonyl-CoA
-
651169
Metallosphaera sedula
?
6.4.1.3
GTP + propanoyl-CoA + HCO3-
10% of the activity with ATP
651169
Metallosphaera sedula TH2 / ATCC 51363 / DSM 5348
GDP + phosphate + (S)-methylmalonyl-CoA
-
651169
Metallosphaera sedula TH2 / ATCC 51363 / DSM 5348
?
6.4.1.3
UTP + propanoyl-CoA + HCO3-
40% of the activity with ATP
651169
Metallosphaera sedula
UDP + phosphate + (S)-methylmalonyl-CoA
-
651169
Metallosphaera sedula
?
6.4.1.3
UTP + propanoyl-CoA + HCO3-
40% of the activity with ATP
651169
Metallosphaera sedula TH2 / ATCC 51363 / DSM 5348
UDP + phosphate + (S)-methylmalonyl-CoA
-
651169
Metallosphaera sedula TH2 / ATCC 51363 / DSM 5348
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
6.4.1.2
multimer
most likely (alpha,beta,gamma)4 composition, two large subunits of 57000 Da: biotin carboxylase (alpha subunit) and carboxyl transferase (gamma subunit), 18600 Da biotin carrier protein (beta subunit)
Chloroflexus aurantiacus
6.4.1.2
multimer
most likely (alpha,beta,gamma)4 composition, two large subunits of 57000 Da: biotin carboxylase (alpha subunit) and carboxyl transferase (gamma subunit), 18600 Da biotin carrier protein (beta subunit)
Sulfuracidifex metallicus
6.4.1.2
multimer
most likely (alpha,beta,gamma)4 composition, two large subunits of 57000 Da: biotin carboxylase (alpha subunit) and carboxyl transferase (gamma subunit), 18600 Da biotin carrier protein (beta subunit)
Metallosphaera sedula
6.4.1.3
dodecamer
alpha, beta, gamma, 4 * 57000 + 4 * 57000 + 4 * 18600, SDS-PAGE, biotin carboxylase (alpha subunit), carboxyl transferase (beta subunit) and biotin carrier protein (gamma subunit)
Metallosphaera sedula
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
6.4.1.2
75
-
-
Metallosphaera sedula
6.4.1.3
75
-
-
Metallosphaera sedula
Temperature Range [°C] (protein specific)
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
6.4.1.2
40
85
-
Metallosphaera sedula
6.4.1.3
40
85
-
Metallosphaera sedula
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
6.4.1.2
2
8
acetyl-CoA
pH 7.5, 65°C
Metallosphaera sedula
6.4.1.3
2
8
propanoyl-CoA
pH 7.5, 65°C
Metallosphaera sedula
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.4.1.2
7.5
-
-
Metallosphaera sedula
6.4.1.3
7.5
-
-
Metallosphaera sedula
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
6.4.1.2
6
8
-
Metallosphaera sedula
6.4.1.3
6
8.5
-
Metallosphaera sedula