BRENDA - Enzyme Database

Kinetic characterization, structure modelling studies and crystallization of Trypanosoma brucei enolase

Hannaert, V.; Albert, M.A.; Rigden, D.J.; da Silva Giotto, M.T.; Thiemann, O.; Garratt, R.C.; Van Roy, J.; Opperdoes, F.R.; Michels, P.A.; Eur. J. Biochem. 270, 3205-3213 (2003)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
4.2.1.11
expression in Escherichia coli of a his-tagged recombinant enzyme
Trypanosoma brucei
Crystallization (Commentary)
EC Number
Crystallization
Organism
4.2.1.11
hanging drop method
Trypanosoma brucei
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.2.1.11
0.054
-
2-phospho-D-glycerate
pH 7.6, 25șC
Trypanosoma brucei
4.2.1.11
0.244
-
phosphoenolpyruvate
pH 7.6, 25șC
Trypanosoma brucei
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
4.2.1.11
cytosol
-
Trypanosoma brucei
5829
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
4.2.1.11
Mg2+
essential for activity, high concentrations are inhibitory
Trypanosoma brucei
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.11
46461
-
x * 46461, calculated from amino-acid sequence
Trypanosoma brucei
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.2.1.11
Trypanosoma brucei
Q9NDH8
-
-
Purification (Commentary)
EC Number
Commentary
Organism
4.2.1.11
using affinity chromatography to isolate a his-tagged recombinant enzyme followed by cleavage of the tag
Trypanosoma brucei
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [”mol/min/mg]
Specific Activity Maximum [”mol/min/mg]
Commentary
Organism
4.2.1.11
85
-
pH 7.6, 25șC
Trypanosoma brucei
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.11
2-phospho-D-glycerate
-
651157
Trypanosoma brucei
phosphoenolpyruvate
-
651157
Trypanosoma brucei
r
Subunits
EC Number
Subunits
Commentary
Organism
4.2.1.11
?
x * 46461, calculated from amino-acid sequence
Trypanosoma brucei
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.2.1.11
7.7
-
25șC
Trypanosoma brucei
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
4.2.1.11
7.3
8
activity decreases steadily, 60% of the activity remains at pH 7.0 and pH 8.4
Trypanosoma brucei
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.11
expression in Escherichia coli of a his-tagged recombinant enzyme
Trypanosoma brucei
Crystallization (Commentary) (protein specific)
EC Number
Crystallization
Organism
4.2.1.11
hanging drop method
Trypanosoma brucei
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.2.1.11
0.054
-
2-phospho-D-glycerate
pH 7.6, 25șC
Trypanosoma brucei
4.2.1.11
0.244
-
phosphoenolpyruvate
pH 7.6, 25șC
Trypanosoma brucei
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
4.2.1.11
cytosol
-
Trypanosoma brucei
5829
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
4.2.1.11
Mg2+
essential for activity, high concentrations are inhibitory
Trypanosoma brucei
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.11
46461
-
x * 46461, calculated from amino-acid sequence
Trypanosoma brucei
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.11
using affinity chromatography to isolate a his-tagged recombinant enzyme followed by cleavage of the tag
Trypanosoma brucei
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [”mol/min/mg]
Specific Activity Maximum [”mol/min/mg]
Commentary
Organism
4.2.1.11
85
-
pH 7.6, 25șC
Trypanosoma brucei
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.11
2-phospho-D-glycerate
-
651157
Trypanosoma brucei
phosphoenolpyruvate
-
651157
Trypanosoma brucei
r
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
4.2.1.11
?
x * 46461, calculated from amino-acid sequence
Trypanosoma brucei
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.2.1.11
7.7
-
25șC
Trypanosoma brucei
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
4.2.1.11
7.3
8
activity decreases steadily, 60% of the activity remains at pH 7.0 and pH 8.4
Trypanosoma brucei