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Literature summary extracted from
- Kinetic characterization, structure modelling studies and crystallization of Trypanosoma brucei enolase (2003), Eur. J. Biochem., 270, 3205-3213.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 4.2.1.11 | expression in Escherichia coli of a his-tagged recombinant enzyme | Trypanosoma brucei |
Crystallization (Commentary)
| EC Number | Crystallization (Comment) | Organism |
|---|---|---|
| 4.2.1.11 | hanging drop method | Trypanosoma brucei |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.2.1.11 | 0.054 | - |
2-phospho-D-glycerate | pH 7.6, 25ºC | Trypanosoma brucei |  |
| 4.2.1.11 | 0.244 | - |
phosphoenolpyruvate | pH 7.6, 25ºC | Trypanosoma brucei | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 4.2.1.11 | cytosol | - |
Trypanosoma brucei | 5829 | - |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.2.1.11 | Mg2+ | essential for activity, high concentrations are inhibitory | Trypanosoma brucei | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.11 | 46461 | - |
x * 46461, calculated from amino-acid sequence | Trypanosoma brucei |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.2.1.11 | Trypanosoma brucei | Q9NDH8 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.2.1.11 | using affinity chromatography to isolate a his-tagged recombinant enzyme followed by cleavage of the tag | Trypanosoma brucei |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.11 | 85 | - |
pH 7.6, 25ºC | Trypanosoma brucei |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.2.1.11 | 2-phospho-D-glycerate | - |
Trypanosoma brucei | phosphoenolpyruvate | - |
r | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 4.2.1.11 | ? | x * 46461, calculated from amino-acid sequence | Trypanosoma brucei |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 4.2.1.11 | 2-phospho-D-glycerate hydrolase | - |
Trypanosoma brucei |
| 4.2.1.11 | enolase | - |
Trypanosoma brucei |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.11 | 7.7 | - |
25ºC | Trypanosoma brucei |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.11 | 7.3 | 8 | activity decreases steadily, 60% of the activity remains at pH 7.0 and pH 8.4 | Trypanosoma brucei |
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Release 2023.1 (January 2023)
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