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Literature summary extracted from

  • Nakatani, M.; Ezaki, S.; Atomi, H.; Imanaka, T.
    Substrate recognition and fidelity of strand joining by an archaeal DNA ligase (2002), Eur. J. Biochem., 269, 650-656.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.5.1.1 expression in Escherichia coli Thermococcus kodakarensis

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.5.1.1 Ca2+ inhibition above 40 mM Thermococcus kodakarensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.5.1.1 0.0014
-
 nicked DNA 80°C, pH 8.0 Thermococcus kodakarensis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.5.1.1 Ca2+ 9% of activity with Mg2+, maximal activity at 5 mM, inhibition above 40 mM Thermococcus kodakarensis
6.5.1.1 Mg2+ divalent cation required for activity, maximal activity at 15 mM Thermococcus kodakarensis
6.5.1.1 Mn2+ 65% of activity with Mg2+, maximal activity at 25 mM Thermococcus kodakarensis
6.5.1.1 Sr2+ 40% of activity with Mg2+, maximal activity at 25 mM Thermococcus kodakarensis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.5.1.1 ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m Thermococcus kodakarensis
-
 AMP + diphosphate + (deoxyribonucleotide)m+n
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.5.1.1 Thermococcus kodakarensis
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.5.1.1 ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m
-
 Thermococcus kodakarensis AMP + diphosphate + (deoxyribonucleotide)m+n
-
 ?
6.5.1.1 ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m proper base pairing at the 3' side of the nick is necessary for efficient ligation Thermococcus kodakarensis AMP + diphosphate + (deoxyribonucleotide)m+n
-
 ?
6.5.1.1 ATP + nicked DNA
-
 Thermococcus kodakarensis AMP + diphosphate + ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.5.1.1 ligTK
-
 Thermococcus kodakarensis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.5.1.1 0.11
-
 nicked DNA 80°C, pH 8.0 Thermococcus kodakarensis