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Literature summary extracted from

  • Nachon, F.; Nicolet, Y.; Viguie, N.; Masson, P.; Fontecilla-Camps, J.C.; Lockridge, O.
    Engineering of a monomeric and low-glycosylated form of human butyrylcholinesterase: expression, purification, characterization and crystallization (2002), Eur. J. Biochem., 269, 630-637.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.8 monomeric enzyme, lacking four out of nine N-glycosylation sites and the C-terminal oligomerization domain, expressed in CHO cells Homo sapiens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.1.1.8 monomeric enzyme, lacking four out of nine N-glycosylation sites and the C-terminal oligomerization domain, hanging drop vapor diffusion method Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
3.1.1.8 additional information mutant with only 5 N-glycosylation sites Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.8 0.0256
-
 butyryl thiocholine iodide pH 7, mutant with only 5 N-glycosylation sites Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.8 70000 75000 SDS-PAGE, recombinant enzyme Homo sapiens
3.1.1.8 85000
-
 native enzyme, SDS-PAGE Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.8 Homo sapiens
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.1.8 glycoprotein nine N-glycosylation sites Homo sapiens

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.8
-
 Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.8 butyrylthiocholine iodide + H2O
-
 Homo sapiens thiocholine iodide + butyrate
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.1.8 535
-
 butyryl thiocholine iodide pH 7, mutant with only 5 N-glycosylation sites Homo sapiens