Literature summary extracted from
Rother, D.; Poppe, L.; Viergutz, S.; Langer, B.; Retey, J.
Characterization of the active site of histidine ammonia-lyase from Pseudomonas putida (2001), Eur. J. Biochem., 268, 6011-6019.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
4.3.1.3 |
expression in Escherichia coli |
Pseudomonas putida |
Protein Variants
EC Number |
Protein Variants |
Comment |
Organism |
---|
4.3.1.3 |
C273A |
20% of wild-type kcat |
Pseudomonas putida |
4.3.1.3 |
C273A/E414A |
no activity |
Pseudomonas putida |
4.3.1.3 |
C273A/E414Q |
0.06% of wild-type kcat |
Pseudomonas putida |
4.3.1.3 |
C273A/F329A |
0.2% of wild-type kcat |
Pseudomonas putida |
4.3.1.3 |
C273A/H83L |
no activity |
Pseudomonas putida |
4.3.1.3 |
C273A/N195A |
0.02% of wild-type kcat |
Pseudomonas putida |
4.3.1.3 |
C273A/Q277A |
0.2% of wild-type kcat |
Pseudomonas putida |
4.3.1.3 |
C273A/R283I |
0.9% of wild-type kcat |
Pseudomonas putida |
4.3.1.3 |
C273A/R283K |
0.01% of wild-type kcat |
Pseudomonas putida |
4.3.1.3 |
C273A/Y280F |
0.4% of wild-type kcat |
Pseudomonas putida |
4.3.1.3 |
C273A/Y53F |
no activity |
Pseudomonas putida |
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
4.3.1.3 |
L-cysteine |
10 mM, irreversible inactivation |
Pseudomonas putida |
|
KM Value [mM]
EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Comment |
Organism |
Structure |
---|
4.3.1.3 |
3.9 |
- |
L-histidine |
25°C, pH 9.3 |
Pseudomonas putida |
|
Natural Substrates/ Products (Substrates)
EC Number |
Natural Substrates |
Organism |
Comment (Nat. Sub.) |
Natural Products |
Comment (Nat. Pro.) |
Rev. |
Reac. |
---|
4.3.1.3 |
L-histidine |
Pseudomonas putida |
- |
urocanate + NH3 |
- |
r |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
4.3.1.3 |
Pseudomonas putida |
P21310 |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
4.3.1.3 |
recombinant histidase |
Pseudomonas putida |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
4.3.1.3 |
L-histidine |
- |
Pseudomonas putida |
urocanate + NH3 |
- |
r |
|
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
4.3.1.3 |
HAL |
- |
Pseudomonas putida |
Turnover Number [1/s]
EC Number |
Turnover Number Minimum [1/s] |
Turnover Number Maximum [1/s] |
Substrate |
Comment |
Organism |
Structure |
---|
4.3.1.3 |
86 |
- |
L-histidine |
25°C, pH 9.3 |
Pseudomonas putida |
|