EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
6.3.2.10 | acetate | activation of mutant K202A | Escherichia coli | |
6.3.2.10 | Butyrate | activation of mutant K202A | Escherichia coli | |
6.3.2.10 | ethylamine | slight activation of mutant K202A | Escherichia coli | |
6.3.2.10 | formate | slight activation of mutant K202A | Escherichia coli | |
6.3.2.10 | propionate | strong activation of mutant K202A | Escherichia coli | |
6.3.2.10 | Propylamine | slight activation of mutant K202A | Escherichia coli |
EC Number | Cloned (Comment) | Organism |
---|---|---|
6.3.2.10 | overexpression of wild-type and MurF mutant K202A as His-tagged enzymes in strain JM83 | Escherichia coli |
6.3.2.13 | expresision of His-tagged MurE in Escherichia coli | Escherichia coli |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
6.3.2.10 | K202A | site-directed mutagenesis, exchange of highly conserved lysine residue leads to highly reduced activity, activity can be rescued best by addition of propionate or other short-chain carboxylic acids, but only in a small extent by amines | Escherichia coli |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
6.3.2.10 | acetate | inhibition of wild-type enzyme | Escherichia coli | |
6.3.2.10 | Butylamine | inhibition of wild-type and mutant K202A; inhibition of wild-type enzyme | Escherichia coli | |
6.3.2.10 | Butyrate | slight inhibition of wild-type enzyme | Escherichia coli | |
6.3.2.10 | ethylamine | inhibition of wild-type enzyme | Escherichia coli | |
6.3.2.10 | formate | inhibition of wild-type enzyme | Escherichia coli | |
6.3.2.10 | methylamine | inhibition of wild-type enzyme | Escherichia coli | |
6.3.2.10 | propionate | inhibition of wild-type enzyme | Escherichia coli | |
6.3.2.10 | Propylamine | inhibition of wild-type enzyme | Escherichia coli | |
6.3.2.13 | Butylamine | 500 mM, 88% inhibition | Escherichia coli | |
6.3.2.13 | Butyrate | 500 mM, 44% inhibition | Escherichia coli | |
6.3.2.13 | ethylamine | 500 mM, 73% inhibition | Escherichia coli | |
6.3.2.13 | methylamine | 500 mM, 80% inhibition | Escherichia coli | |
6.3.2.13 | Propylamine | 500 mM, 87% inhibition | Escherichia coli |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
6.3.2.13 | cytoplasm | - |
Escherichia coli | 5737 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
6.3.2.10 | Mg2+ | required | Escherichia coli |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.3.2.10 | ATP + UDP-N-acetylmuramoyl-L-Ala-D-Glu-L-Lys + D-Ala-D-Ala | Escherichia coli | - |
ADP + phosphate + UDP-N-acetylmuramoyl-L-Ala-D-Glu-L-Lys-D-Ala-D-Ala | - |
? | |
6.3.2.13 | ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + meso-2,6-diaminoheptanedioate | Escherichia coli | - |
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diamino-heptanedioate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
6.3.2.10 | Escherichia coli | - |
- |
- |
6.3.2.13 | Escherichia coli | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
6.3.2.10 | recombinant wild-type and mutant enzyme from strain JM83 | Escherichia coli |
6.3.2.13 | recombinant MurE | Escherichia coli |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
6.3.2.10 | 0.006 | - |
purified recombinant mutant K202A, in presence of butylamine | Escherichia coli |
6.3.2.10 | 0.016 | - |
purified recombinant mutant K202A | Escherichia coli |
6.3.2.10 | 0.032 | - |
purified recombinant mutant K202A, in presence of propylamine | Escherichia coli |
6.3.2.10 | 0.046 | - |
purified recombinant mutant K202A, in presence of ethylamine | Escherichia coli |
6.3.2.10 | 0.052 | - |
purified recombinant mutant K202A, in presence of formate | Escherichia coli |
6.3.2.10 | 0.437 | - |
purified recombinant mutant K202A, in presence of butyrate | Escherichia coli |
6.3.2.10 | 0.511 | - |
purified recombinant mutant K202A, in presence of acetate | Escherichia coli |
6.3.2.10 | 0.55 | - |
purified recombinant wild-type enzyme, in presence of methylamine | Escherichia coli |
6.3.2.10 | 0.71 | - |
purified recombinant wild-type enzyme, in presence of propylamine | Escherichia coli |
6.3.2.10 | 0.72 | - |
purified recombinant wild-type enzyme, in presence of butylamine | Escherichia coli |
6.3.2.10 | 0.76 | - |
purified recombinant wild-type enzyme, in presence of propionate | Escherichia coli |
6.3.2.10 | 0.769 | - |
purified recombinant mutant K202A, in presence of propionate | Escherichia coli |
6.3.2.10 | 0.8 | - |
purified recombinant wild-type enzyme, in presence of ethylamine | Escherichia coli |
6.3.2.10 | 0.88 | - |
purified recombinant wild-type enzyme, in presence of acetate | Escherichia coli |
6.3.2.10 | 0.93 | - |
purified recombinant wild-type enzyme, in presence of formate | Escherichia coli |
6.3.2.10 | 1.24 | - |
purified recombinant wild-type enzyme, in presence of butyrate | Escherichia coli |
6.3.2.10 | 1.27 | - |
purified recombinant wild-type enzyme | Escherichia coli |
6.3.2.13 | 0.0047 | - |
K224A mutant | Escherichia coli |
6.3.2.13 | 0.078 | - |
K224A mutant, in the presence of 500 mM propionate | Escherichia coli |
6.3.2.13 | 1.24 | - |
recombinant wild-type MurE | Escherichia coli |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.3.2.10 | ATP + UDP-N-acetylmuramoyl-L-Ala-D-Glu-L-Lys + D-Ala-D-Ala | - |
Escherichia coli | ADP + phosphate + UDP-N-acetylmuramoyl-L-Ala-D-Glu-L-Lys-D-Ala-D-Ala | - |
? | |
6.3.2.13 | ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + meso-2,6-diaminoheptanedioate | - |
Escherichia coli | ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diamino-heptanedioate | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
6.3.2.10 | D-Ala-D-Ala-adding enzyme | - |
Escherichia coli |
6.3.2.10 | Synthetase, uridine diphosphoacetylmuramoylpentapeptide | - |
Escherichia coli |
6.3.2.10 | UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine synthetase | - |
Escherichia coli |
6.3.2.10 | UDP-N-acetylmuramoylalanyl-D-glutamyl-lysine-D-alanyl-D-alanine ligase | - |
Escherichia coli |
6.3.2.10 | UDPacetylmuramoylpentapeptide synthetase | - |
Escherichia coli |
6.3.2.10 | Uridine diphosphoacetylmuramoylpentapeptide synthetase | - |
Escherichia coli |
6.3.2.13 | MurE | - |
Escherichia coli |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
6.3.2.10 | 37 | - |
assay at | Escherichia coli |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
6.3.2.10 | 8.6 | - |
assay at | Escherichia coli |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
6.3.2.10 | ATP | - |
Escherichia coli |