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Literature summary extracted from

  • Hirschberg, H.J.H.B.; Simons, J.W.F.A.; Dekker, N.; Egmond, M.R.
    Cloning, expression, purification and characterization of patatin, a novel phospholipase A (2001), Eur. J. Biochem., 268, 5037-5044.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.4 expressed in Escherichia coli Solanum tuberosum

Protein Variants

EC Number Protein Variants Comment Organism
3.1.1.4 D192A no enzymatic activity Solanum tuberosum
3.1.1.4 H175N 10% of wild-type activity Solanum tuberosum
3.1.1.4 H288N as active as wild-type enzyme Solanum tuberosum
3.1.1.4 K17A no enzymatic activity Solanum tuberosum
3.1.1.4 S10C 29% of wild-type activity Solanum tuberosum
3.1.1.4 S10T 25% of wild-type activity Solanum tuberosum
3.1.1.4 S234C no enzymatic activity Solanum tuberosum
3.1.1.4 S234T no enzymatic activity Solanum tuberosum
3.1.1.4 S54A no enzymatic activity Solanum tuberosum
3.1.1.4 S54C 1% of wild-type activity Solanum tuberosum
3.1.1.4 S54T no enzymatic activity Solanum tuberosum

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.4 methyl-4-nitrophenyl-octylphosphonate inactivates at 100fold molar excess Solanum tuberosum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.4 56000
-
 SDS-PAGE Solanum tuberosum

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.4 Solanum tuberosum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.4
-
 Solanum tuberosum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.4 tuber
-
 Solanum tuberosum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.4 15
-
 4-nitrophenylcaprate as substrate Solanum tuberosum
3.1.1.4 109
-
 1,2-dinonanoyl-sn-glycero-3-phosphocholine as substrate Solanum tuberosum
3.1.1.4 127
-
 1,2-diocytanoyl-sn-glycero-3-phosphocholine as substrate Solanum tuberosum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.4 1,2-dinonanoyl-sn-glycero-3-phosphocholine + H2O
-
 Solanum tuberosum ?
-
 ?
3.1.1.4 1,2-dioctanoyl-sn-glycero-3-phosphocholine + H2O
-
 Solanum tuberosum 1-octanoyl-sn-glycero-3-phosphocholine + octanoate
-
 ?
3.1.1.4 4-nitrophenyl caprate + H2O
-
 Solanum tuberosum 4-nitrophenol + capric acid
-
 ?
3.1.1.4 additional information enzyme is active towards mono-acyl-glycerolphosphocholines, increasing activity with increasing chain lengths from C12 to C16 Solanum tuberosum ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.4 patatin
-
 Solanum tuberosum