EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.4.24.28 | G8C/N60C | double mutation | Geobacillus stearothermophilus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.4.24.28 | guanidine hydrochloride | competitive inhibitor | Geobacillus stearothermophilus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.4.24.28 | Geobacillus stearothermophilus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.4.24.28 | wild-type and mutant enzymes | Geobacillus stearothermophilus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.24.28 | N-(3-[2-furyl]acryloyl)-Gly-Leu amide + H2O | - |
Geobacillus stearothermophilus | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.4.24.28 | Neutral protease | - |
Geobacillus stearothermophilus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.4.24.28 | 60 | - |
activity toward N-(3-[2-furyl]acryloyl)-Gly-Leu amide is 4 times higher than at 25°C | Geobacillus stearothermophilus |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.4.24.28 | 25 | 65 | - |
Geobacillus stearothermophilus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.4.24.28 | 92.5 | - |
G8C/N60C double mutation extremely thermostable, half-life 35.9 min | Geobacillus stearothermophilus |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.24.28 | 1000 | - |
guanidine hydrochloride | pH 7.5, 25°C | Geobacillus stearothermophilus |