BRENDA - Enzyme Database

Purification and characterization of the first archaeal aconitase from the thermoacidophilic Sulfolobus acidocaldarius

Uhrigshardt, H.; Walden, M.; John, H.; Anemuller, S.; Eur. J. Biochem. 268, 1760-1771 (2001)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
4.2.1.3
Fluorocitrate
-
Sulfolobus acidocaldarius
4.2.1.3
trans-aconitate
-
Sulfolobus acidocaldarius
4.2.1.3
tricarballylate
-
Sulfolobus acidocaldarius
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.2.1.3
0.108
-
cis-aconitate
pH 8.4, 75°C
Sulfolobus acidocaldarius
4.2.1.3
0.37
-
isocitrate
pH 8.4, 75°C
Sulfolobus acidocaldarius
4.2.1.3
2.9
-
citrate
pH 8.4, 75°C
Sulfolobus acidocaldarius
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
4.2.1.3
cytosol
-
Sulfolobus acidocaldarius
5829
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
4.2.1.3
Iron
the enzyme hosts an interconvertible [3Fe-4S] cluster
Sulfolobus acidocaldarius
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.3
96000
-
x * 96000, SDS-PAGE
Sulfolobus acidocaldarius
4.2.1.3
145000
-
gel filtration
Sulfolobus acidocaldarius
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.2.1.3
Sulfolobus acidocaldarius
-
-
-
Oxidation Stability
EC Number
Oxidation Stability
Organism
4.2.1.3
high oxygen sensitivity
Sulfolobus acidocaldarius
Purification (Commentary)
EC Number
Commentary
Organism
4.2.1.3
-
Sulfolobus acidocaldarius
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.2.1.3
55
-
-
Sulfolobus acidocaldarius
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.3
cis-aconitate + H2O
-
651095
Sulfolobus acidocaldarius
citrate
-
651095
Sulfolobus acidocaldarius
?
4.2.1.3
isocitrate
-
651095
Sulfolobus acidocaldarius
?
-
651095
Sulfolobus acidocaldarius
?
Subunits
EC Number
Subunits
Commentary
Organism
4.2.1.3
?
x * 96000, SDS-PAGE
Sulfolobus acidocaldarius
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
4.2.1.3
75
-
-
Sulfolobus acidocaldarius
Temperature Range [°C]
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
4.2.1.3
50
90
50°C: about 40% of maximal activity, 90°C: about 45% of maximal activity
Sulfolobus acidocaldarius
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
4.2.1.3
80
-
1 h, stable
Sulfolobus acidocaldarius
4.2.1.3
95
-
half-life: 14 min
Sulfolobus acidocaldarius
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.2.1.3
7.4
-
-
Sulfolobus acidocaldarius
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
4.2.1.3
6.4
8.2
pH 6.4: about 60% of maximal activity, pH 8.2: about 75% of maximal activity
Sulfolobus acidocaldarius
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
4.2.1.3
Fluorocitrate
-
Sulfolobus acidocaldarius
4.2.1.3
trans-aconitate
-
Sulfolobus acidocaldarius
4.2.1.3
tricarballylate
-
Sulfolobus acidocaldarius
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.2.1.3
0.108
-
cis-aconitate
pH 8.4, 75°C
Sulfolobus acidocaldarius
4.2.1.3
0.37
-
isocitrate
pH 8.4, 75°C
Sulfolobus acidocaldarius
4.2.1.3
2.9
-
citrate
pH 8.4, 75°C
Sulfolobus acidocaldarius
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
4.2.1.3
cytosol
-
Sulfolobus acidocaldarius
5829
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
4.2.1.3
Iron
the enzyme hosts an interconvertible [3Fe-4S] cluster
Sulfolobus acidocaldarius
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.3
96000
-
x * 96000, SDS-PAGE
Sulfolobus acidocaldarius
4.2.1.3
145000
-
gel filtration
Sulfolobus acidocaldarius
Oxidation Stability (protein specific)
EC Number
Oxidation Stability
Organism
4.2.1.3
high oxygen sensitivity
Sulfolobus acidocaldarius
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.3
-
Sulfolobus acidocaldarius
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.2.1.3
55
-
-
Sulfolobus acidocaldarius
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.3
cis-aconitate + H2O
-
651095
Sulfolobus acidocaldarius
citrate
-
651095
Sulfolobus acidocaldarius
?
4.2.1.3
isocitrate
-
651095
Sulfolobus acidocaldarius
?
-
651095
Sulfolobus acidocaldarius
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
4.2.1.3
?
x * 96000, SDS-PAGE
Sulfolobus acidocaldarius
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
4.2.1.3
75
-
-
Sulfolobus acidocaldarius
Temperature Range [°C] (protein specific)
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
4.2.1.3
50
90
50°C: about 40% of maximal activity, 90°C: about 45% of maximal activity
Sulfolobus acidocaldarius
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
4.2.1.3
80
-
1 h, stable
Sulfolobus acidocaldarius
4.2.1.3
95
-
half-life: 14 min
Sulfolobus acidocaldarius
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.2.1.3
7.4
-
-
Sulfolobus acidocaldarius
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
4.2.1.3
6.4
8.2
pH 6.4: about 60% of maximal activity, pH 8.2: about 75% of maximal activity
Sulfolobus acidocaldarius