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Literature summary extracted from

  • Kramer, R.A.; Zandwijken, D.; Egmond, M.R.; Dekker, N.
    In vitro folding, purification and characterization of Escherichia coli outer membrane protease OmpT (2000), Eur. J. Biochem., 267, 885-893.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.23.49 overexpressed without its signal sequence in Escherichia coli K-12 strain DH5alpha using a T7 system Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
3.4.23.49 G216K/K217G recombinant ompT variant in order to abolish autoproteolysis Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.23.49 diisopropylfluorophosphate significant inhibition only at high concentrations Escherichia coli
3.4.23.49 phenylmethylsulfonyl fluoride poor inhibitor, only at high concentrations Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.23.49 0.0003
-
 o-aminobenzoyl-Ala-Arg-Arg-Ala-3-nitrotyrosine-NH2 pH 6.5, 37°C, wild-type, purified from outer membrane Escherichia coli
3.4.23.49 0.0004
-
 o-aminobenzoyl-Ala-Arg-Arg-Ala-3-nitrotyrosine-NH2 pH 6.5, 37°C, wild-type, recombinant expressed Escherichia coli
3.4.23.49 0.0011
-
 o-aminobenzoyl-Ala-Arg-Arg-Ala-3-nitrotyrosine-NH2 pH 6.5, 37°C, variant G216K/K217G Escherichia coli
3.4.23.49 0.53
-
 IAA-Arg-Arg-p-nitroanilide pH 6.5, 37°C, wild-type, purified from outer membrane Escherichia coli
3.4.23.49 0.58
-
 IAA-Arg-Arg-p-nitroanilide pH 6.5, 37°C, wild-type, recombinant expressed Escherichia coli
3.4.23.49 0.79
-
 IAA-Arg-Arg-p-nitroanilide pH 6.5, 37°C, variant G216K/K217G Escherichia coli

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.23.49 membrane integral membrane protease, outer membrane, membrane bound Escherichia coli 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.23.49 29000
-
 SDS-PAGE Escherichia coli
3.4.23.49 33480
-
 ESI-MS,positive-ion-mode electrospray ionization mass spectrometry Escherichia coli
3.4.23.49 33500
-
 calculated from amino acid sequence Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.23.49 additional information Escherichia coli cleaves peptides between two consecutive basic amino acids ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.23.49 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.23.49
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.23.49 2-aminobenzoyl-AKKA-3-[(2,4-dinitrophenyl)amino]-L-alanyl-Gly + H2O
-
 Escherichia coli ?
-
 ?
3.4.23.49 2-aminobenzoyl-ARKA-3-[(2,4-dinitrophenyl)amino]-L-alanyl-Gly + H2O
-
 Escherichia coli ?
-
 ?
3.4.23.49 2-aminobenzoyl-IRRA-3-[(2,4-dinitrophenyl)amino]-L-alanyl-Gly + H2O
-
 Escherichia coli 2-aminobenzoyl-Ile-Arg + Arg-Ala-3-[(2,4-dinitrophenyl)amino]-L-alanyl-Gly
-
 ?
3.4.23.49 aminobenzoyl-Ala-Lys-Lys-Ala-3-(dinitrophenyl)diaminopropionic acid-Gly + H2O
-
 Escherichia coli ?
-
 ?
3.4.23.49 IAA-Arg-Arg-p-nitroanilide + H2O
-
 Escherichia coli ?
-
 ?
3.4.23.49 additional information cleaves peptides between two consecutive basic amino acids Escherichia coli ?
-
 ?
3.4.23.49 o-aminobenzoyl-Ala-Arg-Arg-Ala-3-nitrotyrosine-NH2 + H2O
-
 Escherichia coli ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.23.49 ompT
-
 Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.23.49 18
-
 IAA-Arg-Arg-p-nitroanilide pH 6.5, 37°C, variant G216K/K217G Escherichia coli
3.4.23.49 24
-
 IAA-Arg-Arg-p-nitroanilide pH 6.5, 37°C, wild-type, purified from outer membrane and wild-type, recombinant expressed Escherichia coli
3.4.23.49 96
-
 o-aminobenzoyl-Ala-Arg-Arg-Ala-3-nitrotyrosine-NH2 pH 6.5, 37°C, variant G216K/K217G Escherichia coli
3.4.23.49 2280
-
 o-aminobenzoyl-Ala-Arg-Arg-Ala-3-nitrotyrosine-NH2 pH 6.5, 37°C, wild-type, purified from outer membrane Escherichia coli
3.4.23.49 2460
-
 o-aminobenzoyl-Ala-Arg-Arg-Ala-3-nitrotyrosine-NH2 pH 6.5, 37°C, wild-type, recombinant expressed in Escherichia coli Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.23.49 6.5
-
-
 Escherichia coli

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.23.49 5 8
-
 Escherichia coli