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Literature summary extracted from

  • Nairn, J.; Duncan, D.; Price, N.E.; Kelly, S.M.; Fothergill-Gilmore, L.A.; Uhrinova, S.; Barlow, P.N.; Rigden, D.J.; Price, N.C.
    Characterization of active-site mutants of Schizosaccharomyces pombe phosphoglycerate mutase. Elucidation of the roles of amino acids involved in substrate binding and catalysis (2000), Eur. J. Biochem., 267, 7065-7074.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
5.4.2.11 2,3-bisphosphoglycerate
-
 Schizosaccharomyces pombe
5.4.2.11 2-phosphoglycollate slight activation of phosphatase activity Schizosaccharomyces pombe

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.4.2.11 expression in Saccharomyces cerevisiae Schizosaccharomyces pombe

Protein Variants

EC Number Protein Variants Comment Organism
5.4.2.11 E93D less than 1% of wild-type activity Schizosaccharomyces pombe
5.4.2.11 R120Q 59% of wild-type activity Schizosaccharomyces pombe
5.4.2.11 R120Q/R121Q 62% of wild-type activity Schizosaccharomyces pombe
5.4.2.11 R121Q 12% of wild-type activity Schizosaccharomyces pombe
5.4.2.11 R14Q less than 1% of wild-type activity Schizosaccharomyces pombe
5.4.2.11 R94Q 1% of wild-type activity Schizosaccharomyces pombe
5.4.2.11 S62A loss of activity during purification Schizosaccharomyces pombe

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.4.2.11 0.58
-
 3-phosphoglycerate
-
 Schizosaccharomyces pombe
5.4.2.11 1.25
-
 3-phosphoglycerate E93D mutant Schizosaccharomyces pombe
5.4.2.11 1.54
-
 3-phosphoglycerate R14Q mutant Schizosaccharomyces pombe
5.4.2.11 2.64
-
 3-phosphoglycerate R120Q mutant Schizosaccharomyces pombe
5.4.2.11 4.9
-
 3-phosphoglycerate R121Q mutant Schizosaccharomyces pombe
5.4.2.11 5.26
-
 3-phosphoglycerate R120Q/R121Q double mutant Schizosaccharomyces pombe
5.4.2.11 7.46
-
 3-phosphoglycerate R94Q mutant Schizosaccharomyces pombe

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.4.2.11 2-phospho-D-glycerate Schizosaccharomyces pombe
-
 3-phospho-D-glycerate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
5.4.2.11 Schizosaccharomyces pombe
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.4.2.11 2-phospho-D-glycerate
-
 Schizosaccharomyces pombe 3-phospho-D-glycerate
-
 r
5.4.2.11 3-Phosphoglycerate
-
 Schizosaccharomyces pombe 2-Phosphoglycerate
-
 ?