BRENDA - Enzyme Database

Hev b 9, an enolase and a new cross-reactive allergen from hevea latex and molds. Purification, characterization, cloning and expression

Wagner, S.; Breiteneder, H.; Simon-Nobbe, B.; Susani, M.; Krebitz, M.; Niggemann, B.; Brehler, R.; Scheiner, O.; Hoffmann-Sommergruber, K.; Eur. J. Biochem. 267, 7006-7014 (2000)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
4.2.1.11
expression in Escherichia coli of a his-tagged recombinant enzyme
Hevea brasiliensis
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.11
47600
-
x * 47600, calculated from amino acid sequence
Hevea brasiliensis
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.2.1.11
Hevea brasiliensis
Q9LEJ0
clone RRIM 600
-
Purification (Commentary)
EC Number
Commentary
Organism
4.2.1.11
using affinity chromatography to isolate a his-tagged recombinant enzyme, purification from latex using ammonium sulfate precipitation and gel filtration chromatography
Hevea brasiliensis
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
4.2.1.11
latex
-
Hevea brasiliensis
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.2.1.11
22
-
pH 7.4, 25ºC
Hevea brasiliensis
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.11
2-phospho-D-glycerate
-
651087
Hevea brasiliensis
phosphoenolpyruvate
-
651087
Hevea brasiliensis
r
Subunits
EC Number
Subunits
Commentary
Organism
4.2.1.11
?
x * 47600, calculated from amino acid sequence
Hevea brasiliensis
pI Value
EC Number
Organism
Commentary
pI Value Maximum
pI Value
4.2.1.11
Hevea brasiliensis
calculated from amino acid sequence
5.9
5.6
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.11
expression in Escherichia coli of a his-tagged recombinant enzyme
Hevea brasiliensis
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.11
47600
-
x * 47600, calculated from amino acid sequence
Hevea brasiliensis
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.11
using affinity chromatography to isolate a his-tagged recombinant enzyme, purification from latex using ammonium sulfate precipitation and gel filtration chromatography
Hevea brasiliensis
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
4.2.1.11
latex
-
Hevea brasiliensis
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.2.1.11
22
-
pH 7.4, 25ºC
Hevea brasiliensis
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.11
2-phospho-D-glycerate
-
651087
Hevea brasiliensis
phosphoenolpyruvate
-
651087
Hevea brasiliensis
r
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
4.2.1.11
?
x * 47600, calculated from amino acid sequence
Hevea brasiliensis
pI Value (protein specific)
EC Number
Organism
Commentary
pI Value Maximum
pI Value
4.2.1.11
Hevea brasiliensis
calculated from amino acid sequence
5.9
5.6