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Literature summary extracted from

  • Wagner, S.; Breiteneder, H.; Simon-Nobbe, B.; Susani, M.; Krebitz, M.; Niggemann, B.; Brehler, R.; Scheiner, O.; Hoffmann-Sommergruber, K.
    Hev b 9, an enolase and a new cross-reactive allergen from hevea latex and molds. Purification, characterization, cloning and expression (2000), Eur. J. Biochem., 267, 7006-7014.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.11 expression in Escherichia coli of a his-tagged recombinant enzyme Hevea brasiliensis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.11 47600
-
 x * 47600, calculated from amino acid sequence Hevea brasiliensis

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.11 Hevea brasiliensis Q9LEJ0 clone RRIM 600
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.11 using affinity chromatography to isolate a his-tagged recombinant enzyme, purification from latex using ammonium sulfate precipitation and gel filtration chromatography Hevea brasiliensis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.1.11 latex
-
 Hevea brasiliensis
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.11 22
-
 pH 7.4, 25ºC Hevea brasiliensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.11 2-phospho-D-glycerate
-
 Hevea brasiliensis phosphoenolpyruvate
-
 r

Subunits

EC Number Subunits Comment Organism
4.2.1.11 ? x * 47600, calculated from amino acid sequence Hevea brasiliensis

Synonyms

EC Number Synonyms Comment Organism
4.2.1.11 enolase
-
 Hevea brasiliensis

pI Value

EC Number Organism Comment pI Value Maximum pI Value
4.2.1.11 Hevea brasiliensis calculated from amino acid sequence 5.9 5.6