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Literature summary extracted from

  • Elleby, B.; Sjoeblom, B.; Tu, C.; Silverman, D.N.; Lindskog, S.
    Enhancement of catalytic efficiency by the combination of site-specific mutations in a carbonic anhydrase-related protein (2000), Eur. J. Biochem., 267, 5908-5915.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.1 expressed in Escherichia coli BL21(DE3) Mus musculus

Protein Variants

EC Number Protein Variants Comment Organism
4.2.1.1 E115Q site-directed mutagenesis, results in increased activity Mus musculus
4.2.1.1 E92Q site-directed mutagenesis, results in increased activity Mus musculus
4.2.1.1 R117H site-directed mutagenesis, replacement reconstructs a zinc-binding site and changes a catalytically inactive murine carbonic anhydrase-related protein to an active carbonic anhydrase Mus musculus
4.2.1.1 R94H site-directed mutagenesis, replacement reconstructs a zinc-binding site and changes a catalytically inactive murine carbonic anhydrase-related protein to an active carbonic anhydrase Mus musculus
4.2.1.1 R94H/E92Q site-directed mutagenesis Mus musculus
4.2.1.1 R94H/E92Q/I121V site-directed mutagenesis Mus musculus
4.2.1.1 R94H/E92Q/I121V/I143V/I200T site-directed mutagenesis Mus musculus
4.2.1.1 R94H/E92Q/I143V/I200T site-directed mutagenesis Mus musculus
4.2.1.1 R94H/E92Q/I200T site-directed mutagenesis Mus musculus

General Stability

EC Number General Stability Organism
4.2.1.1 guanidine hydrochloride causes denaturation Mus musculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.1 Cl-
-
 Mus musculus
4.2.1.1 CN-
-
 Mus musculus
4.2.1.1 I-
-
 Mus musculus
4.2.1.1 N3-
-
 Mus musculus
4.2.1.1 NCO-
-
 Mus musculus
4.2.1.1 NO3-
-
 Mus musculus
4.2.1.1 SCN-
-
 Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.1 1.6
-
 CO2 pH 9.0, 25°C, mutant R94H/E92Q/I121V/I143V/I200T Mus musculus
4.2.1.1 5.6
-
 CO2 pH 9.0, 25°C, mutant R94H/E92Q/I143V/I200T Mus musculus
4.2.1.1 5.9
-
 CO2 pH 9.0, 25°C, mutant R94H/E92Q/I143V Mus musculus
4.2.1.1 13
-
 CO2 pH 9.0, 25°C, mutant R94H/E92Q/I121V Mus musculus
4.2.1.1 15
-
 CO2 pH 9.0, 25°C, mutant R94H/E92Q/I200T Mus musculus
4.2.1.1 19
-
 CO2 pH 9.0, 25°C, mutant R94H Mus musculus
4.2.1.1 22
-
 CO2 pH 9.0, 25°C, mutant R94H/E92Q Mus musculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.1 H2CO3 Mus musculus
-
 CO2 + H2O
-
 r

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.1 Mus musculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.1
-
 Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.1 H2CO3
-
 Mus musculus CO2 + H2O
-
 r

Synonyms

EC Number Synonyms Comment Organism
4.2.1.1 carbonic anhydrase
-
 Mus musculus
4.2.1.1 carbonic anhydrase-related protein
-
 Mus musculus
4.2.1.1 CARP
-
 Mus musculus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2.1.1 0.012
-
 CO2 pH 9.0, 25°C, mutant R94H Mus musculus
4.2.1.1 0.019
-
 CO2 pH 9.0, 25°C, mutant R94H/E92Q/I143V Mus musculus
4.2.1.1 0.023
-
 CO2 pH 9.0, 25°C, mutant R94H/E92Q Mus musculus
4.2.1.1 0.034
-
 CO2 pH 9.0, 25°C, mutant R94H/E92Q/I121V Mus musculus
4.2.1.1 0.038
-
 CO2 pH 9.0, 25°C, mutant R94H/E92Q/I143V/I200T Mus musculus
4.2.1.1 0.039
-
 CO2 pH 9.0, 25°C, mutant R94H/E92Q/I121V/I143V/I200T Mus musculus
4.2.1.1 0.046
-
 CO2 pH 9.0, 25°C, mutant R94H/E92Q/I200T Mus musculus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.2.1.1 6 9
-
 Mus musculus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.2.1.1 0.0065
-
 CN- pH 7.0, 10°C, double mutant R94H/E92Q Mus musculus
4.2.1.1 0.0071
-
 NCO- pH 7.0, 10°C, double mutant R94H/E92Q Mus musculus
4.2.1.1 0.12
-
 SCN- pH 7.0, 10°C, double mutant R94H/E92Q Mus musculus
4.2.1.1 0.27
-
 N3- pH 7.0, 10°C, double mutant R94H/E92Q Mus musculus
4.2.1.1 12.1
-
 NO3- pH 7.0, 10°C, double mutant R94H/E92Q Mus musculus
4.2.1.1 21.4
-
 I- pH 7.0, 10°C, double mutant R94H/E92Q Mus musculus
4.2.1.1 222
-
 Cl- pH 7.0, 10°C, double mutant R94H/E92Q Mus musculus