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Literature summary extracted from

  • Durinx, C.; Lambeir, A.M.; Bosmans, E.; Falmagne, J.B.; Berghmans, R.; Haemers, A.; Scharpe, S.; De Meester, I.
    Molecular characterization of dipeptidyl peptidase activity in serum. Soluble CD26/dipeptidyl peptidase IV is responsible for the release of X-Pro dipeptides (2000), Eur. J. Biochem., 267, 5608-5613.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.14.5 expression in human K562 erythroleukemia cell line, secretion to the medium of the recombinant enzyme Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.14.5 di{4-[(erythrocarbonyl)methylaminoacrbonyl]-phenyl} 1-((S)-prolyl)pyrrolidine-2(R,S)-phosphonate irreversible Homo sapiens
3.4.14.5 N-isoleucyl-3-fluoro-pyrrolidine reversible Homo sapiens
3.4.14.5 Zn2+ competitive Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.14.5 plasma membrane
-
 Homo sapiens 5886
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.14.5 110000
-
 x * 110000, enzyme from serum and seminal plasma, SDS-PAGE Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.4.14.5 Homo sapiens P27487 T-cell enzyme
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.14.5 glycoprotein heavily glycosylated, differing in the pattern dependent on the enzyme origin Homo sapiens

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.14.5 native from serum and seminal plasma, recombinant from K562 cell dulture medium Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.14.5 B-cell
-
 Homo sapiens
-
3.4.14.5 endothelium
-
 Homo sapiens
-
3.4.14.5 seminal plasma
-
 Homo sapiens
-
3.4.14.5 serum
-
 Homo sapiens
-
3.4.14.5 T-cell
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.14.5 41.9
-
 purified native enzyme from serum Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.14.5 Gly-Pro-4-methoxy-2-naphthylamide fluorogenic substrate Homo sapiens Gly-Pro + 4-methoxy-2-naphthylamine
-
 ?
3.4.14.5 Gly-Pro-4-nitroanilide + H2O
-
 Homo sapiens Gly-Pro + 4-nitroaniline
-
 ?
3.4.14.5 additional information the enzyme binds with high affinity to adenosine deaminase, EC 3.5.4.4 Homo sapiens ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.14.5 ? x * 110000, enzyme from serum and seminal plasma, SDS-PAGE Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
3.4.14.5 CD26 enzyme is identical with adenosine deaminase binding protein, CD26 Homo sapiens
3.4.14.5 dipeptidyl peptidase IV
-
 Homo sapiens
3.4.14.5 DPPIV
-
 Homo sapiens
3.4.14.5 More the enzyme is not identical with attractin Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.14.5 37
-
 assay at Homo sapiens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.14.5 65
-
 5% remaining activity after 100 min, slightly differing thermal stability for the 3 different purified enzyme forms from serum, seminal plasma and recombinant from K562 cells Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.14.5 8
-
 about Homo sapiens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.14.5 5 8 no activity below pH 5.0, optimum at pH 8.0 Homo sapiens