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Literature summary extracted from

  • Desai, N.A.; Shankar, V.
    Purification and characterization of the single-strand-specific and guanylic-acid-preferential deoxyribonuclease activity of the extracellular nuclease from Basidiobolus haptosporus (2000), Eur. J. Biochem., 267, 5123-5135.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.21.1 2-mercaptoethanol 70% inhibition at 1 mM Basidiobolus haptosporus
3.1.21.1 3'-AMP
-
 Basidiobolus haptosporus
3.1.21.1 5'-deoxy-GMP competitive, product inhibition Basidiobolus haptosporus
3.1.21.1 5'-GDP
-
 Basidiobolus haptosporus
3.1.21.1 5'-GMP
-
 Basidiobolus haptosporus
3.1.21.1 5'-GTP
-
 Basidiobolus haptosporus
3.1.21.1 Ag2+
-
 Basidiobolus haptosporus
3.1.21.1 Al3+
-
 Basidiobolus haptosporus
3.1.21.1 diphosphate
-
 Basidiobolus haptosporus
3.1.21.1 DTT 80% inhibition at 1 mM Basidiobolus haptosporus
3.1.21.1 Fe3+
-
 Basidiobolus haptosporus
3.1.21.1 guanidinium hydrochloride over 80% inhibition at 0.5 M Basidiobolus haptosporus
3.1.21.1 Hg2+
-
 Basidiobolus haptosporus
3.1.21.1 KCl stimulating between 25 and 50 mM, inhibitory above 50 mM Basidiobolus haptosporus
3.1.21.1 additional information the phosphate residue is responsible for the inhibitory effect of guanosine 5'-nucleotides Basidiobolus haptosporus
3.1.21.1 NaCl stimulating between 25 and 50 mM, inhibitory above 50 mM Basidiobolus haptosporus
3.1.21.1 phosphate
-
 Basidiobolus haptosporus
3.1.21.1 SDS over 80% inhibition at 0.04% w/v Basidiobolus haptosporus
3.1.21.1 Urea 20% inhibition at 4 M Basidiobolus haptosporus
3.1.21.1 Zn2+
-
 Basidiobolus haptosporus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.21.1 extracellular
-
 Basidiobolus haptosporus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.21.1 KCl stimulating between 25 and 50 mM, inhibitory above 50 mM Basidiobolus haptosporus
3.1.21.1 additional information metalloprotein, enzyme has no obligate requirement for metal ions to be active, nor is its activity stimulated by addition of metal ions Basidiobolus haptosporus
3.1.21.1 NaCl stimulating between 25 and 50 mM, inhibitory above 50 mM Basidiobolus haptosporus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.21.1 30000
-
 1 * 30000, deglycosylated enzyme, SDS-PAGE, 42000-47000, glycosylated enzyme, SDS-PAGE Basidiobolus haptosporus
3.1.21.1 41000
-
 gel filtration Basidiobolus haptosporus

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.1.21.1 dimethyl formamide 20% decrease in activity at 40% v/v Basidiobolus haptosporus
3.1.21.1 dimethyl sulfoxide 20% decrease in activity at 15% v/v Basidiobolus haptosporus
3.1.21.1 formamide stable to at 50% v/v Basidiobolus haptosporus

Organism

EC Number Organism UniProt Comment Textmining
3.1.21.1 Basidiobolus haptosporus
-
 multifunctional enzyme
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.21.1 glycoprotein 15% carbohydrate content Basidiobolus haptosporus

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.21.1 6057fold to homogeneity Basidiobolus haptosporus

Reaction

EC Number Reaction Comment Organism Reaction ID
3.1.21.1 endonucleolytic cleavage to 5'-phosphodinucleotide and 5'-phosphooligonucleotide end-products preference for single-stranded DNA and 3'AMP, guanylic acid preferential endo-exonuclease, mode of action, overview Basidiobolus haptosporus
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.21.1 14540
-
 purified enzyme, substrate RNA Basidiobolus haptosporus
3.1.21.1 597200
-
 purified enzyme, substrate ssDNA Basidiobolus haptosporus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.21.1 3'AMP + H2O phosphomonoesterase activity Basidiobolus haptosporus adenosine + phosphate
-
 ?
3.1.21.1 dsDNA + H2O very low activity Basidiobolus haptosporus ?
-
 ?
3.1.21.1 additional information substrate specificity, 5'-AMP, bis(4-nitrophenyl)phosphate, and 4-nitrophenyl phosphate are no substrates, preferential liberation of 5'-deoxy-GMP, no cleavage of C-linkages, (dC)10 is no substrate, no liberation of 5'-deoxy-CMP Basidiobolus haptosporus ?
-
 ?
3.1.21.1 phage M13 DNA + H2O endolytically cleavage generating single base nicks Basidiobolus haptosporus ?
-
 ?
3.1.21.1 RNA + H2O low activity Basidiobolus haptosporus ?
-
 ?
3.1.21.1 ssDNA + H2O
-
 Basidiobolus haptosporus ?
-
 ?
3.1.21.1 supercoiled pUC18 DNA + H2O endolytically cleavage generating single base nicks Basidiobolus haptosporus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.21.1 monomer 1 * 30000, deglycosylated enzyme, SDS-PAGE, 42000-47000, glycosylated enzyme, SDS-PAGE Basidiobolus haptosporus

Synonyms

EC Number Synonyms Comment Organism
3.1.21.1 More see also EC 3.1.30.1 Basidiobolus haptosporus
3.1.21.1 nuclease Bh1
-
 Basidiobolus haptosporus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.21.1 60
-
-
 Basidiobolus haptosporus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.21.1 60
-
 15 min, stable Basidiobolus haptosporus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.21.1 8.5
-
-
 Basidiobolus haptosporus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.1.21.1 0.0028
-
 5'-GTP pH 8.5, 37°C Basidiobolus haptosporus
3.1.21.1 0.0058
-
 5'-GDP pH 8.5, 37°C Basidiobolus haptosporus
3.1.21.1 0.0283
-
 5'-GMP pH 8.5, 37°C Basidiobolus haptosporus
3.1.21.1 0.04
-
 5'-deoxy-GMP pH 8.5, 37°C Basidiobolus haptosporus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.1.21.1 Basidiobolus haptosporus isoelectric focusing
-
 6.8