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Literature summary extracted from

  • Dobers, J.; Grams, S.; Reutter, W.; Fan, H.
    Roles of cysteines in rat dipeptidyl peptidase IV/CD26 in processing and proteolytic activity (2000), Eur. J. Biochem., 267, 5093-5100.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.14.5 expression of wild-type and mutants in CHO cells Rattus norvegicus

Protein Variants

EC Number Protein Variants Comment Organism
3.4.14.5 C299G site-directed mutagenesis, similar to the wild-type enzyme Rattus norvegicus
3.4.14.5 C326G site-directed mutagenesis, total inhibition of surface expression, catalytically inactive, half-life of the mutant enzyme is reduced by over 90% Rattus norvegicus
3.4.14.5 C337S site-directed mutagenesis, weak expression, reduced activity, half-life of the mutant enzyme is reduced by over 90% Rattus norvegicus
3.4.14.5 C383G site-directed mutagenesis, similar to the wild-type enzyme Rattus norvegicus
3.4.14.5 C395S site-directed mutagenesis, similar to the wild-type enzyme Rattus norvegicus
3.4.14.5 C445S site-directed mutagenesis, total inhibition of surface expression, catalytically inactive, half-life of the mutant enzyme is reduced by over 90% Rattus norvegicus
3.4.14.5 C448S site-directed mutagenesis, total inhibition of surface expression, catalytically inactive, half-life of the mutant enzyme is reduced by over 90% Rattus norvegicus
3.4.14.5 C455G site-directed mutagenesis, weak expression, reduced activity, half-life of the mutant enzyme is reduced by over 90% Rattus norvegicus
3.4.14.5 C473S site-directed mutagenesis, weak expression, reduced activity, half-life of the mutant enzyme is reduced by over 90% Rattus norvegicus
3.4.14.5 C552S site-directed mutagenesis, weak expression, reduced activity, half-life of the mutant enzyme is reduced by over 90% Rattus norvegicus
3.4.14.5 C650G site-directed mutagenesis, similar to the wild-type enzyme Rattus norvegicus
3.4.14.5 C763S site-directed mutagenesis, similar to the wild-type enzyme Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.14.5 additional information
-
 additional information processing kinetics of wild-type and mutants Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.14.5 endoplasmic reticulum
-
 Rattus norvegicus 5783
-
3.4.14.5 Golgi apparatus
-
 Rattus norvegicus 5794
-
3.4.14.5 membrane type II transmembrane glycoprotein, cell surface Rattus norvegicus 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.14.5 110000
-
 2 * 110000, wild-type enzyme Rattus norvegicus
3.4.14.5 210000
-
 wild-type enzyme Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
3.4.14.5 no activity in Cricetulus griseus
-
 chinese hamster, ovary cells
-
3.4.14.5 Rattus norvegicus P14740
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.14.5 glycoprotein type II transmembrane glycoprotein Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.14.5 liver
-
 Rattus norvegicus
-
3.4.14.5 lymphocyte
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.14.5 Gly-Pro-4-nitroanilide + H2O
-
 Rattus norvegicus Gly-Pro + 4-nitroaniline
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.14.5 dimer 2 * 110000, wild-type enzyme Rattus norvegicus
3.4.14.5 More domain structure involving cysteine residues and disulfide bridges, structure-function relationship, overview, cysteine residues 326, 337,445, 448, 455, 473, and 552 are essential for the correct folding and intracellular trafficking of the enzyme, structure of wild-type compared to the mutant enzymes Rattus norvegicus

Synonyms

EC Number Synonyms Comment Organism
3.4.14.5 CD26 dipeptidyl-peptidase IV is identical with the adenosine deaminase binding protein CD26 on lymphocytes Rattus norvegicus
3.4.14.5 dipeptidyl peptidase IV
-
 Rattus norvegicus
3.4.14.5 DPPIV
-
 Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.14.5 7.4
-
 assay at Rattus norvegicus