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Literature summary extracted from

  • Sawada, K.; Ogawa, A.; Ozawa, T.; Sumitomo, N.; Hatada, Y.; Kobayashi, T.; Ito, S.
    Nucleotide and amino-acid sequences of a new-type pectate lyase from an alkaliphilic strain of Bacillus (2000), Eur. J. Biochem., 267, 1510-1515.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.2.2 EDTA 1 mM, pH 7.5, 10 min at 30°C, activity is completely abolished, fully recovered by adding 0.8 mM CaCl2 Bacillus sp. (in: Bacteria)

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.2.2.2 extracellular
-
 Bacillus sp. (in: Bacteria)
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.2.2 Ca2+ absolute requirement Bacillus sp. (in: Bacteria)

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.2.2 33000
-
 x * 33000, SDS-PAGE Bacillus sp. (in: Bacteria)

Organism

EC Number Organism UniProt Comment Textmining
4.2.2.2 Bacillus sp. (in: Bacteria) Q9XDT2
-
-
4.2.2.2 Bacillus sp. (in: Bacteria) KSM-P15 Q9XDT2
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.2.2
-
 Bacillus sp. (in: Bacteria)

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.2.2 culture medium
-
 Bacillus sp. (in: Bacteria)
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.2.2 pectin relative degradation rates of citrus pectin with methylation degrees 31%, 63% and 94% is 124%, 73% and 9% compared to polygalacturonic acid Bacillus sp. (in: Bacteria) ?
-
 ?
4.2.2.2 pectin relative degradation rates of citrus pectin with methylation degrees 31%, 63% and 94% is 124%, 73% and 9% compared to polygalacturonic acid Bacillus sp. (in: Bacteria) KSM-P15 ?
-
 ?
4.2.2.2 polygalacturonic acid
-
 Bacillus sp. (in: Bacteria) unsaturated oligogalacturonides
-
 ?
4.2.2.2 polygalacturonic acid
-
 Bacillus sp. (in: Bacteria) KSM-P15 unsaturated oligogalacturonides
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.2.2 ? x * 33000, SDS-PAGE Bacillus sp. (in: Bacteria)

Synonyms

EC Number Synonyms Comment Organism
4.2.2.2 pectate lyase
-
 Bacillus sp. (in: Bacteria)
4.2.2.2 pectate lyase E
-
 Bacillus sp. (in: Bacteria)
4.2.2.2 pectate transeliminase
-
 Bacillus sp. (in: Bacteria)
4.2.2.2 Pel-15E
-
 Bacillus sp. (in: Bacteria)

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.2.2 50 55
-
 Bacillus sp. (in: Bacteria)

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
4.2.2.2 40 50 40°C: about 50% of maximal activity, 60°C: about 25% of maximal activity Bacillus sp. (in: Bacteria)

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.2.2 additional information
-
 Ca2+ increases thermal stability of the enzyme slightly Bacillus sp. (in: Bacteria)
4.2.2.2 60
-
 pH 7.5, stable in presence of Ca2+ Bacillus sp. (in: Bacteria)

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.2.2 10.5
-
-
 Bacillus sp. (in: Bacteria)

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.2.2.2 8.5 11.5 pH 8.5: about 35% of maximal activity, pH 11.5: 75% of maximal activity Bacillus sp. (in: Bacteria)

pI Value

EC Number Organism Comment pI Value Maximum pI Value
4.2.2.2 Bacillus sp. (in: Bacteria) isoelectric focusing, pH range: 3-10
-
 9.2