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Literature summary extracted from
- Purification and biochemical characterization of a novel cysteine protease of Entamoeba histolytica (1999), Eur. J. Biochem., 266, 170-180.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.22.35 | dithiothreitol | activates | Entamoeba histolytica |  |
| 3.4.22.35 | sulfhydryl compounds | activate | Entamoeba histolytica | |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.22.35 | 4-[(2S,3S)-carboxyoxiran-2-ylcarbonyl-L-leucylamido]butylguanidine | i.e. trans-epoxysuccinyl-L-leucylamido-(4-guanidino)butane or E64 | Entamoeba histolytica | |
| 3.4.22.35 | EDTA | slight inhibition at 10 mM | Entamoeba histolytica | |
| 3.4.22.35 | iodoacetamide | - |
Entamoeba histolytica | |
| 3.4.22.35 | additional information | no inhibition by pepstatin and phenylmethylsulfonyl fluoride | Entamoeba histolytica | |
| 3.4.22.35 | N-ethylmaleimide | - |
Entamoeba histolytica | |
| 3.4.22.35 | Phenanthroline | slight inhibition at 10 mM | Entamoeba histolytica | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.4.22.35 | cytoplasm | - |
Entamoeba histolytica | 5737 | - |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.4.22.35 | 66000 | - |
native PAGE | Entamoeba histolytica |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.22.35 | protein + H2O | Entamoeba histolytica | - |
peptides | - |
? | |
| 3.4.22.35 | protein + H2O | Entamoeba histolytica HM1:IMSS | - |
peptides | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.22.35 | Entamoeba histolytica | - |
and its derivative clone A | - |
| 3.4.22.35 | Entamoeba histolytica HM1:IMSS | - |
and its derivative clone A | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.22.35 | 15fold, to homogeneity | Entamoeba histolytica |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.4.22.35 | additional information | the enzyme is translocated to the amoebic surface upon the interaction of trophozoites with red blood cells, the enzyme participates thus in erythrophagocytosis | Entamoeba histolytica | - |
| 3.4.22.35 | trophozoite | - |
Entamoeba histolytica | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.4.22.35 | 2800 | - |
purified enzyme | Entamoeba histolytica |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.22.35 | Gelatin + H2O | - |
Entamoeba histolytica | ? | - |
? | |
| 3.4.22.35 | Gelatin + H2O | - |
Entamoeba histolytica HM1:IMSS | ? | - |
? | |
| 3.4.22.35 | additional information | no activity with N-benzyloxycarbonyl-Val-Lys-Met-7-amido-4-methylcoumarin and succinyl-LLVY-7-amido-4-methylcoumarin | Entamoeba histolytica | ? | - |
? | |
| 3.4.22.35 | additional information | no activity with N-benzyloxycarbonyl-Val-Lys-Met-7-amido-4-methylcoumarin and succinyl-LLVY-7-amido-4-methylcoumarin | Entamoeba histolytica HM1:IMSS | ? | - |
? | |
| 3.4.22.35 | N-benzyloxycarbonyl-L-arginyl-7-amido-4-methylcoumarin + H2O | 17% of the activity with N-benzyloxycarbonyl-L-arginyl-L-arginyl-7-amido-4-methylcoumarin | Entamoeba histolytica | N-benzyloxycarbonyl-L-arginine + 7-amino-4-methylcoumarin | - |
? | |
| 3.4.22.35 | N-benzyloxycarbonyl-L-arginyl-7-amido-4-methylcoumarin + H2O | 17% of the activity with N-benzyloxycarbonyl-L-arginyl-L-arginyl-7-amido-4-methylcoumarin | Entamoeba histolytica HM1:IMSS | N-benzyloxycarbonyl-L-arginine + 7-amino-4-methylcoumarin | - |
? | |
| 3.4.22.35 | N-benzyloxycarbonyl-L-arginyl-L-arginyl-7-amido-4-methylcoumarin + H2O | - |
Entamoeba histolytica | N-benzyloxycarbonyl-L-arginyl-L-arginine + 7-amino-4-methylcoumarin | - |
? | |
| 3.4.22.35 | N-benzyloxycarbonyl-L-arginyl-L-arginyl-7-amido-4-methylcoumarin + H2O | - |
Entamoeba histolytica HM1:IMSS | N-benzyloxycarbonyl-L-arginyl-L-arginine + 7-amino-4-methylcoumarin | - |
? | |
| 3.4.22.35 | N-benzyloxycarbonyl-L-phenylalanyl-L-arginyl-7-amido-4-methylcoumarin + H2O | 7% of the activity with N-benzyloxycarbonyl-L-arginyl-L-arginyl-7-amido-4-methylcoumarin | Entamoeba histolytica | N-benzyloxycarbonyl-L-phenylalanyl-L-arginine + 7-amino-4-methylcoumarin | - |
? | |
| 3.4.22.35 | protein + H2O | - |
Entamoeba histolytica | peptides | - |
? | |
| 3.4.22.35 | protein + H2O | - |
Entamoeba histolytica HM1:IMSS | peptides | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.4.22.35 | monomer | 1 * 50000-60000, 3 forms, SDS-PAGE | Entamoeba histolytica |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.4.22.35 | Entamoeba histolytica cysteine protease | - |
Entamoeba histolytica |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.22.35 | 37 | - |
assay at | Entamoeba histolytica |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.22.35 | 6.5 | - |
- |
Entamoeba histolytica |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.22.35 | 5 | 7.5 | - |
Entamoeba histolytica |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 3.4.22.35 | Entamoeba histolytica | isoelectric focusing | - |
6.7 |
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